Restriction map for
pFN21AE6693
(Linear) (Six-base) MAPSORT of: KIEE6693.seq Check: 11 from: 1 to: 954
KIEE6693.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AccI GT'mk_AC
Cuts at: 0 569 954
Size: 569 385
AclI AA'CG_TT
Cuts at: 0 183 954
Size: 183 771
AflIII A'CryG_T
Cuts at: 0 366 954
Size: 366 588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 820 954
Size: 820 134
AlwNI CAG_nnn'CTG
Cuts at: 0 619 954
Size: 619 335
AvaI C'yCGr_G
Cuts at: 0 818 954
Size: 818 136
BanI G'GyrC_C
Cuts at: 0 385 575 954
Size: 385 190 379
Fragments arranged by size:
385 379 190
BanII G_rGCy'C
Cuts at: 0 473 954
Size: 473 481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 721 755 954
Size: 721 34 199
Fragments arranged by size:
721 199 34
BglI GCCn_nnn'nGGC
Cuts at: 0 884 954
Size: 884 70
BglII A'GATC_T
Cuts at: 0 50 954
Size: 50 904
Bme1580I G_kGCm'C
Cuts at: 0 578 954
Size: 578 376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 954
Size: 276 678
BsaAI yAC'GTr
Cuts at: 0 367 954
Size: 367 587
BsaHI Gr'CG_yC
Cuts at: 0 322 954
Size: 322 632
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 954
Size: 124 30 800
Fragments arranged by size:
800 124 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 97 115 954
Size: 94 3 18 839
Fragments arranged by size:
839 94 18 3
BseYI C'CCAG_C
Cuts at: 0 206 954
Size: 206 748
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 615 954
Size: 506 109 339
Fragments arranged by size:
506 339 109
BsiHKAI G_wGCw'C
Cuts at: 0 431 473 954
Size: 431 42 481
Fragments arranged by size:
481 431 42
Bsp1286I G_dGCh'C
Cuts at: 0 431 473 578 954
Size: 431 42 105 376
Fragments arranged by size:
431 376 105 42
BsrDI GCAATG_nn'
Cuts at: 0 797 802 954
Size: 797 5 152
Fragments arranged by size:
797 152 5
BssSI C'ACGA_G
Cuts at: 0 653 954
Size: 653 301
BstYI r'GATC_y
Cuts at: 0 50 954
Size: 50 904
BtgI C'CryG_G
Cuts at: 0 282 480 606 829 954
Size: 282 198 126 223 125
Fragments arranged by size:
282 223 198 126 125
BtsI GCAGTG_nn'
Cuts at: 0 131 332 954
Size: 131 201 622
Fragments arranged by size:
622 201 131
ClaI AT'CG_AT
Cuts at: 0 731 954
Size: 731 223
EaeI y'GGCC_r
Cuts at: 0 249 348 560 885 954
Size: 249 99 212 325 69
Fragments arranged by size:
325 249 212 99 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 580 954
Size: 580 374
EcoICRI GAG'CTC
Cuts at: 0 471 954
Size: 471 483
EcoO109I rG'GnC_Cy
Cuts at: 0 642 954
Size: 642 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 763 795 954
Size: 124 32 607 32 159
Fragments arranged by size:
607 159 124 32 32
HincII GTy'rAC
Cuts at: 0 297 954
Size: 297 657
MscI TGG'CCA
Cuts at: 0 251 350 887 954
Size: 251 99 537 67
Fragments arranged by size:
537 251 99 67
MslI CAynn'nnrTG
Cuts at: 0 701 954
Size: 701 253
MspA1I CmG'CkG
Cuts at: 0 436 723 909 954
Size: 436 287 186 45
Fragments arranged by size:
436 287 186 45
NcoI C'CATG_G
Cuts at: 0 282 480 954
Size: 282 198 474
Fragments arranged by size:
474 282 198
PstI C_TGCA'G
Cuts at: 0 68 467 954
Size: 68 399 487
Fragments arranged by size:
487 399 68
PvuII CAG'CTG
Cuts at: 0 436 723 909 954
Size: 436 287 186 45
Fragments arranged by size:
436 287 186 45
SacI G_AGCT'C
Cuts at: 0 473 954
Size: 473 481
SbfI CC_TGCA'GG
Cuts at: 0 467 954
Size: 467 487
SfcI C'TryA_G
Cuts at: 0 64 463 811 954
Size: 64 399 348 143
Fragments arranged by size:
399 348 143 64
SmlI C'TyrA_G
Cuts at: 0 255 954
Size: 255 699
StyI C'CwwG_G
Cuts at: 0 282 480 954
Size: 282 198 474
Fragments arranged by size:
474 282 198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 906 954
Size: 906 48
Enzymes that do cut:
AccI AclI AflIII AlfI AlwNI AvaI BanI BanII
BcgI BglI BglII Bme1580I BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BssSI BstYI
BtgI BtsI ClaI EaeI EciI EcoICRI EcoO109I Hin4I
HincII MscI MslI MspA1I NcoI PstI PvuII SacI
SbfI SfcI SmlI StyI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BfrBI BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I
BsaI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraI DraIII
DrdI EagI EarI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI