Restriction map for
pFN21AE6691
(Linear) (Six-base) MAPSORT of: KIEE6691.seq Check: 5186 from: 1 to: 954
KIEE6691.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AccI GT'mk_AC
Cuts at: 0 206 878 954
Size: 206 672 76
Fragments arranged by size:
672 206 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 674 954
Size: 282 392 280
Fragments arranged by size:
392 282 280
AflII C'TTAA_G
Cuts at: 0 564 954
Size: 564 390
AflIII A'CryG_T
Cuts at: 0 350 954
Size: 350 604
ApoI r'AATT_y
Cuts at: 0 161 954
Size: 161 793
AseI AT'TA_AT
Cuts at: 0 332 954
Size: 332 622
BamHI G'GATC_C
Cuts at: 0 483 954
Size: 483 471
BanII G_rGCy'C
Cuts at: 0 188 954
Size: 188 766
BclI T'GATC_A
Cuts at: 0 62 954
Size: 62 892
BfrBI ATG'CAT
Cuts at: 0 858 916 954
Size: 858 58 38
Fragments arranged by size:
858 58 38
BmtI G_CTAG'C
Cuts at: 0 670 954
Size: 670 284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 710 954
Size: 545 165 244
Fragments arranged by size:
545 244 165
BsaI GGTCTCn'nnnn_
Cuts at: 0 395 521 954
Size: 395 126 433
Fragments arranged by size:
433 395 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 545 575 954
Size: 545 30 379
Fragments arranged by size:
545 379 30
BsiHKAI G_wGCw'C
Cuts at: 0 188 954
Size: 188 766
BsmI GAATG_Cn'
Cuts at: 0 110 954
Size: 110 844
Bsp1286I G_dGCh'C
Cuts at: 0 188 954
Size: 188 766
BsrDI GCAATG_nn'
Cuts at: 0 779 954
Size: 779 175
BstAPI GCAn_nnn'nTGC
Cuts at: 0 706 715 954
Size: 706 9 239
Fragments arranged by size:
706 239 9
BstYI r'GATC_y
Cuts at: 0 483 954
Size: 483 471
BstZ17I GTA'TAC
Cuts at: 0 207 879 954
Size: 207 672 75
Fragments arranged by size:
672 207 75
Bsu36I CC'TnA_GG
Cuts at: 0 886 954
Size: 886 68
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 423 458 954
Size: 423 35 496
Fragments arranged by size:
496 423 35
EcoICRI GAG'CTC
Cuts at: 0 186 954
Size: 186 768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 545 674 710 954
Size: 282 263 129 36 244
Fragments arranged by size:
282 263 244 129 36
EcoRV GAT'ATC
Cuts at: 0 153 650 954
Size: 153 497 304
Fragments arranged by size:
497 304 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 545 577 834 866 954
Size: 545 32 257 32 88
Fragments arranged by size:
545 257 88 32 32
HindIII A'AGCT_T
Cuts at: 0 371 954
Size: 371 583
MslI CAynn'nnrTG
Cuts at: 0 113 954
Size: 113 841
NheI G'CTAG_C
Cuts at: 0 666 954
Size: 666 288
NsiI A_TGCA'T
Cuts at: 0 860 918 954
Size: 860 58 36
Fragments arranged by size:
860 58 36
NspI r_CATG'y
Cuts at: 0 354 862 954
Size: 354 508 92
Fragments arranged by size:
508 354 92
PciI A'CATG_T
Cuts at: 0 350 954
Size: 350 604
PstI C_TGCA'G
Cuts at: 0 255 954
Size: 255 699
SacI G_AGCT'C
Cuts at: 0 188 954
Size: 188 766
SexAI A'CCwGG_T
Cuts at: 0 791 954
Size: 791 163
SfcI C'TryA_G
Cuts at: 0 251 954
Size: 251 703
SmlI C'TyrA_G
Cuts at: 0 564 954
Size: 564 390
Enzymes that do cut:
AccI AcuI AflII AflIII ApoI AseI BamHI BanII
BclI BfrBI BmtI BpmI BsaI BsaXI BsiHKAI BsmI
Bsp1286I BsrDI BstAPI BstYI BstZ17I Bsu36I CspCI EcoICRI
Eco57MI EcoRV Hin4I HindIII MslI NheI NsiI NspI
PciI PstI SacI SexAI SfcI SmlI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI