Restriction map for
pFN21AE6690
(Linear) (Six-base) MAPSORT of: KIEE6690.seq Check: 8318 from: 1 to: 897
KIEE6690.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 218 897
Size: 146 72 679
Fragments arranged by size:
679 146 72
AflII C'TTAA_G
Cuts at: 0 390 897
Size: 390 507
AflIII A'CryG_T
Cuts at: 0 213 897
Size: 213 684
AleI CACnn'nnGTG
Cuts at: 0 89 897
Size: 89 808
AlwNI CAG_nnn'CTG
Cuts at: 0 247 358 897
Size: 247 111 539
Fragments arranged by size:
539 247 111
ApoI r'AATT_y
Cuts at: 0 457 485 897
Size: 457 28 412
Fragments arranged by size:
457 412 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 190 223 897
Size: 190 33 674
Fragments arranged by size:
674 190 33
BamHI G'GATC_C
Cuts at: 0 42 897
Size: 42 855
BanII G_rGCy'C
Cuts at: 0 535 897
Size: 535 362
BbsI GAAGACnn'nnnn_
Cuts at: 0 431 897
Size: 431 466
BbvCI CC'TCA_GC
Cuts at: 0 764 897
Size: 764 133
BclI T'GATC_A
Cuts at: 0 372 897
Size: 372 525
BmrI ACTGGGnnnn_n'
Cuts at: 0 675 897
Size: 675 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 897
Size: 521 376
Bpu10I CC'TnA_GC
Cuts at: 0 764 897
Size: 764 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 897
Size: 476 421
BsaBI GATnn'nnATC
Cuts at: 0 377 897
Size: 377 520
BseYI C'CCAG_C
Cuts at: 0 713 897
Size: 713 184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 803 897
Size: 113 690 94
Fragments arranged by size:
690 113 94
Bsp1286I G_dGCh'C
Cuts at: 0 535 897
Size: 535 362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 192 897
Size: 192 705
BsrGI T'GTAC_A
Cuts at: 0 411 897
Size: 411 486
BstYI r'GATC_y
Cuts at: 0 42 378 897
Size: 42 336 519
Fragments arranged by size:
519 336 42
EarI CTCTTCn'nnn_
Cuts at: 0 194 277 897
Size: 194 83 620
Fragments arranged by size:
620 194 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 218 521 897
Size: 146 72 303 376
Fragments arranged by size:
376 303 146 72
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 894 897
Size: 894 3
FspI TGC'GCA
Cuts at: 0 298 897
Size: 298 599
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 655 687 723 755 897
Size: 418 32 205 32 36 32 142
Fragments arranged by size:
418 205 142 36 32 32 32
HindIII A'AGCT_T
Cuts at: 0 158 871 897
Size: 158 713 26
Fragments arranged by size:
713 158 26
MslI CAynn'nnrTG
Cuts at: 0 89 773 897
Size: 89 684 124
Fragments arranged by size:
684 124 89
MspA1I CmG'CkG
Cuts at: 0 67 897
Size: 67 830
NspI r_CATG'y
Cuts at: 0 217 897
Size: 217 680
PasI CC'CwG_GG
Cuts at: 0 172 897
Size: 172 725
PciI A'CATG_T
Cuts at: 0 213 897
Size: 213 684
PfoI T'CCnGG_A
Cuts at: 0 419 897
Size: 419 478
PshAI GACnn'nnGTC
Cuts at: 0 576 897
Size: 576 321
PvuII CAG'CTG
Cuts at: 0 67 897
Size: 67 830
SapI GCTCTTCn'nnn_
Cuts at: 0 194 897
Size: 194 703
SexAI A'CCwGG_T
Cuts at: 0 684 897
Size: 684 213
SfcI C'TryA_G
Cuts at: 0 71 150 202 897
Size: 71 79 52 695
Fragments arranged by size:
695 79 71 52
SmlI C'TyrA_G
Cuts at: 0 390 491 897
Size: 390 101 406
Fragments arranged by size:
406 390 101
StuI AGG'CCT
Cuts at: 0 253 897
Size: 253 644
StyI C'CwwG_G
Cuts at: 0 480 897
Size: 480 417
TatI w'GTAC_w
Cuts at: 0 411 653 897
Size: 411 242 244
Fragments arranged by size:
411 244 242
XmnI GAAnn'nnTTC
Cuts at: 0 8 897
Size: 8 889
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI ApoI BaeI BamHI
BanII BbsI BbvCI BclI BmrI BpmI Bpu10I BpuEI
BsaBI BseYI BsgI Bsp1286I BspMI BsrGI BstYI EarI
Eco57MI FalI FspI Hin4I HindIII MslI MspA1I NspI
PasI PciI PfoI PshAI PvuII SapI SexAI SfcI
SmlI StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BanI BbeI BcgI BciVI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI