Restriction map for
pFN21AE6688
(Linear) (Six-base) MAPSORT of: KIEE6688.seq Check: 917 from: 1 to: 705
KIEE6688.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 109 141 705
Size: 109 32 564
Fragments arranged by size:
564 109 32
ApaI G_GGCC'C
Cuts at: 0 76 389 473 705
Size: 76 313 84 232
Fragments arranged by size:
313 232 84 76
AscI GG'CGCG_CC
Cuts at: 0 11 705
Size: 11 694
AvaI C'yCGr_G
Cuts at: 0 82 88 682 705
Size: 82 6 594 23
Fragments arranged by size:
594 82 23 6
BanI G'GyrC_C
Cuts at: 0 445 507 614 705
Size: 445 62 107 91
Fragments arranged by size:
445 107 91 62
BanII G_rGCy'C
Cuts at: 0 76 89 389 473 705
Size: 76 13 300 84 232
Fragments arranged by size:
300 232 84 76 13
BbeI G_GCGC'C
Cuts at: 0 449 618 705
Size: 449 169 87
Fragments arranged by size:
449 169 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 37 71 705
Size: 37 34 634
Fragments arranged by size:
634 37 34
BglI GCCn_nnn'nGGC
Cuts at: 0 384 468 654 705
Size: 384 84 186 51
Fragments arranged by size:
384 186 84 51
Bme1580I G_kGCm'C
Cuts at: 0 76 389 473 538 705
Size: 76 313 84 65 167
Fragments arranged by size:
313 167 84 76 65
BmrI ACTGGGnnnn_n'
Cuts at: 0 557 705
Size: 557 148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 705
Size: 421 284
BsaHI Gr'CG_yC
Cuts at: 0 446 491 615 705
Size: 446 45 124 90
Fragments arranged by size:
446 124 90 45
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 705
Size: 163 542
BseYI C'CCAG_C
Cuts at: 0 108 472 705
Size: 108 364 233
Fragments arranged by size:
364 233 108
BsiEI CG_ry'CG
Cuts at: 0 178 419 705
Size: 178 241 286
Fragments arranged by size:
286 241 178
Bsp1286I G_dGCh'C
Cuts at: 0 76 89 389 473 538 705
Size: 76 13 300 84 65 167
Fragments arranged by size:
300 167 84 76 65 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 677 705
Size: 677 28
BsrFI r'CCGG_y
Cuts at: 0 153 177 705
Size: 153 24 528
Fragments arranged by size:
528 153 24
BssHII G'CGCG_C
Cuts at: 0 11 168 170 363 421 481 705
Size: 11 157 2 193 58 60 224
Fragments arranged by size:
224 193 157 60 58 11 2
BssSI C'ACGA_G
Cuts at: 0 531 705
Size: 531 174
BstXI CCAn_nnnn'nTGG
Cuts at: 0 570 705
Size: 570 135
BtgI C'CryG_G
Cuts at: 0 381 408 658 705
Size: 381 27 250 47
Fragments arranged by size:
381 250 47 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 324 705
Size: 324 381
EaeI y'GGCC_r
Cuts at: 0 175 203 416 572 655 705
Size: 175 28 213 156 83 50
Fragments arranged by size:
213 175 156 83 50 28
EagI C'GGCC_G
Cuts at: 0 175 416 705
Size: 175 241 289
Fragments arranged by size:
289 241 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 160 666 705
Size: 160 506 39
Fragments arranged by size:
506 160 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 705
Size: 421 284
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 705
Size: 592 113
EcoO109I rG'GnC_Cy
Cuts at: 0 72 73 386 469 705
Size: 72 1 313 83 236
Fragments arranged by size:
313 236 83 72 1
FspI TGC'GCA
Cuts at: 0 322 705
Size: 322 383
HaeII r_GCGC'y
Cuts at: 0 125 274 449 618 705
Size: 125 149 175 169 87
Fragments arranged by size:
175 169 149 125 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 11 43 678 705
Size: 11 32 635 27
Fragments arranged by size:
635 32 27 11
KasI G'GCGC_C
Cuts at: 0 445 614 705
Size: 445 169 91
Fragments arranged by size:
445 169 91
MspA1I CmG'CkG
Cuts at: 0 383 524 620 660 705
Size: 383 141 96 40 45
Fragments arranged by size:
383 141 96 45 40
NaeI GCC'GGC
Cuts at: 0 155 705
Size: 155 550
NarI GG'CG_CC
Cuts at: 0 446 615 705
Size: 446 169 90
Fragments arranged by size:
446 169 90
NgoMIV G'CCGG_C
Cuts at: 0 153 705
Size: 153 552
PfoI T'CCnGG_A
Cuts at: 0 233 705
Size: 233 472
PspOMI G'GGCC_C
Cuts at: 0 72 385 469 705
Size: 72 313 84 236
Fragments arranged by size:
313 236 84 72
PstI C_TGCA'G
Cuts at: 0 133 468 705
Size: 133 335 237
Fragments arranged by size:
335 237 133
SacII CC_GC'GG
Cuts at: 0 384 661 705
Size: 384 277 44
Fragments arranged by size:
384 277 44
SbfI CC_TGCA'GG
Cuts at: 0 468 705
Size: 468 237
SfcI C'TryA_G
Cuts at: 0 129 464 705
Size: 129 335 241
Fragments arranged by size:
335 241 129
SfoI GGC'GCC
Cuts at: 0 447 616 705
Size: 447 169 89
Fragments arranged by size:
447 169 89
SmaI CCC'GGG
Cuts at: 0 90 684 705
Size: 90 594 21
Fragments arranged by size:
594 90 21
StuI AGG'CCT
Cuts at: 0 26 250 598 705
Size: 26 224 348 107
Fragments arranged by size:
348 224 107 26
StyI C'CwwG_G
Cuts at: 0 188 705
Size: 188 517
XmaI C'CCGG_G
Cuts at: 0 88 682 705
Size: 88 594 23
Fragments arranged by size:
594 88 23
Enzymes that do cut:
AloI ApaI AscI AvaI BanI BanII BbeI BcgI
BglI Bme1580I BmrI BpmI BsaHI BseRI BseYI BsiEI
Bsp1286I BspMI BsrFI BssHII BssSI BstXI BtgI BtgZI
EaeI EagI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KasI MspA1I NaeI NarI NgoMIV PfoI PspOMI
PstI SacII SbfI SfcI SfoI SmaI StuI StyI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EarI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI