Restriction map for
pFN21AE6685
(Linear) (Six-base) MAPSORT of: KIEE6685.seq Check: 3416 from: 1 to: 687
KIEE6685.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AccI GT'mk_AC
Cuts at: 0 395 687
Size: 395 292
Acc65I G'GTAC_C
Cuts at: 0 101 687
Size: 101 586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 472 490 687
Size: 286 186 18 197
Fragments arranged by size:
286 197 186 18
AgeI A'CCGG_T
Cuts at: 0 98 687
Size: 98 589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 433 687
Size: 433 254
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 687
Size: 193 32 462
Fragments arranged by size:
462 193 32
AlwNI CAG_nnn'CTG
Cuts at: 0 35 264 498 687
Size: 35 229 234 189
Fragments arranged by size:
234 229 189 35
BanI G'GyrC_C
Cuts at: 0 101 687
Size: 101 586
BanII G_rGCy'C
Cuts at: 0 148 687
Size: 148 539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 71 105 687
Size: 71 34 582
Fragments arranged by size:
582 71 34
BclI T'GATC_A
Cuts at: 0 503 687
Size: 503 184
BglI GCCn_nnn'nGGC
Cuts at: 0 10 687
Size: 10 677
BlpI GC'TnA_GC
Cuts at: 0 68 687
Size: 68 619
Bme1580I G_kGCm'C
Cuts at: 0 29 687
Size: 29 658
BsaWI w'CCGG_w
Cuts at: 0 98 104 335 687
Size: 98 6 231 352
Fragments arranged by size:
352 231 98 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 193 223 687
Size: 85 30 78 30 464
Fragments arranged by size:
464 85 78 30 30
BseYI C'CCAG_C
Cuts at: 0 28 257 687
Size: 28 229 430
Fragments arranged by size:
430 229 28
BsiHKAI G_wGCw'C
Cuts at: 0 674 687
Size: 674 13
Bsp1286I G_dGCh'C
Cuts at: 0 29 148 674 687
Size: 29 119 526 13
Fragments arranged by size:
526 119 29 13
BspHI T'CATG_A
Cuts at: 0 485 687
Size: 485 202
BsrDI GCAATG_nn'
Cuts at: 0 222 687
Size: 222 465
BsrFI r'CCGG_y
Cuts at: 0 98 687
Size: 98 589
BstYI r'GATC_y
Cuts at: 0 134 687
Size: 134 553
BtsI GCAGTG_nn'
Cuts at: 0 189 687
Size: 189 498
EaeI y'GGCC_r
Cuts at: 0 2 687
Size: 2 685
EarI CTCTTCn'nnn_
Cuts at: 0 284 517 687
Size: 284 233 170
Fragments arranged by size:
284 233 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 472 490 687
Size: 286 186 18 197
Fragments arranged by size:
286 197 186 18
EcoO109I rG'GnC_Cy
Cuts at: 0 591 687
Size: 591 96
HaeII r_GCGC'y
Cuts at: 0 209 687
Size: 209 478
KpnI G_GTAC'C
Cuts at: 0 105 687
Size: 105 582
MscI TGG'CCA
Cuts at: 0 4 687
Size: 4 683
MslI CAynn'nnrTG
Cuts at: 0 520 687
Size: 520 167
MspA1I CmG'CkG
Cuts at: 0 67 264 450 687
Size: 67 197 186 237
Fragments arranged by size:
237 197 186 67
PflMI CCAn_nnn'nTGG
Cuts at: 0 35 39 687
Size: 35 4 648
Fragments arranged by size:
648 35 4
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 687
Size: 193 32 462
Fragments arranged by size:
462 193 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 111 143 687
Size: 111 32 544
Fragments arranged by size:
544 111 32
PvuII CAG'CTG
Cuts at: 0 67 264 450 687
Size: 67 197 186 237
Fragments arranged by size:
237 197 186 67
SfcI C'TryA_G
Cuts at: 0 662 687
Size: 662 25
StyI C'CwwG_G
Cuts at: 0 327 440 687
Size: 327 113 247
Fragments arranged by size:
327 247 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 640 672 687
Size: 640 32 15
Fragments arranged by size:
640 32 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 687
Size: 36 651
Enzymes that do cut:
AccI Acc65I AcuI AgeI AlfI AloI AlwNI BanI
BanII BcgI BclI BglI BlpI Bme1580I BsaWI BsaXI
BseYI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BstYI BtsI
EaeI EarI Eco57MI EcoO109I HaeII KpnI MscI MslI
MspA1I PflMI PpiI PsrI PvuII SfcI StyI TstI
XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AhdI AleI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BfrBI BglII
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BseRI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI XmnI ZraI