Restriction map for
pFN21AE6683
(Linear) (Six-base) MAPSORT of: KIEE6683.seq Check: 8423 from: 1 to: 960
KIEE6683.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 683 960
Size: 683 277
AccI GT'mk_AC
Cuts at: 0 98 960
Size: 98 862
Acc65I G'GTAC_C
Cuts at: 0 806 960
Size: 806 154
AclI AA'CG_TT
Cuts at: 0 742 960
Size: 742 218
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 960
Size: 324 636
AlwNI CAG_nnn'CTG
Cuts at: 0 330 960
Size: 330 630
ApaI G_GGCC'C
Cuts at: 0 571 960
Size: 571 389
BanI G'GyrC_C
Cuts at: 0 103 695 806 960
Size: 103 592 111 154
Fragments arranged by size:
592 154 111 103
BanII G_rGCy'C
Cuts at: 0 274 502 571 758 960
Size: 274 228 69 187 202
Fragments arranged by size:
274 228 202 187 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 19 884 960
Size: 19 865 76
Fragments arranged by size:
865 76 19
BfrBI ATG'CAT
Cuts at: 0 637 960
Size: 637 323
BglI GCCn_nnn'nGGC
Cuts at: 0 580 960
Size: 580 380
Bme1580I G_kGCm'C
Cuts at: 0 571 700 960
Size: 571 129 260
Fragments arranged by size:
571 260 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 269 960
Size: 269 691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 262 597 960
Size: 171 91 335 363
Fragments arranged by size:
363 335 171 91
BsaHI Gr'CG_yC
Cuts at: 0 525 960
Size: 525 435
BsaWI w'CCGG_w
Cuts at: 0 20 960
Size: 20 940
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 400 595 625 960
Size: 370 30 195 30 335
Fragments arranged by size:
370 335 195 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 452 841 907 960
Size: 452 389 66 53
Fragments arranged by size:
452 389 66 53
BseYI C'CCAG_C
Cuts at: 0 39 281 420 933 960
Size: 39 242 139 513 27
Fragments arranged by size:
513 242 139 39 27
Bsp1286I G_dGCh'C
Cuts at: 0 274 502 571 700 758 960
Size: 274 228 69 129 58 202
Fragments arranged by size:
274 228 202 129 69 58
BspEI T'CCGG_A
Cuts at: 0 20 960
Size: 20 940
BspHI T'CATG_A
Cuts at: 0 74 362 960
Size: 74 288 598
Fragments arranged by size:
598 288 74
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 683 960
Size: 683 277
BsrBI CCG'CTC
Cuts at: 0 386 960
Size: 386 574
Bsu36I CC'TnA_GG
Cuts at: 0 683 960
Size: 683 277
BtgI C'CryG_G
Cuts at: 0 116 230 482 960
Size: 116 114 252 478
Fragments arranged by size:
478 252 116 114
BtsI GCAGTG_nn'
Cuts at: 0 778 960
Size: 778 182
EarI CTCTTCn'nnn_
Cuts at: 0 262 866 960
Size: 262 604 94
Fragments arranged by size:
604 262 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 262 324 597 960
Size: 171 91 62 273 363
Fragments arranged by size:
363 273 171 91 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 294 960
Size: 294 666
EcoO109I rG'GnC_Cy
Cuts at: 0 567 568 912 960
Size: 567 1 344 48
Fragments arranged by size:
567 344 48 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 960
Size: 198 762
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 69 101 261 293 370 402 595 627
Size: 69 32 160 32 77 32 193 32
Cuts at: 627 960
Size: 333
Fragments arranged by size:
333 193 160 77 69 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 659 960
Size: 659 301
KpnI G_GTAC'C
Cuts at: 0 810 960
Size: 810 150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 960
Size: 725 235
MslI CAynn'nnrTG
Cuts at: 0 352 960
Size: 352 608
MspA1I CmG'CkG
Cuts at: 0 702 804 960
Size: 702 102 156
Fragments arranged by size:
702 156 102
NcoI C'CATG_G
Cuts at: 0 116 230 960
Size: 116 114 730
Fragments arranged by size:
730 116 114
NsiI A_TGCA'T
Cuts at: 0 639 960
Size: 639 321
NspI r_CATG'y
Cuts at: 0 159 960
Size: 159 801
PasI CC'CwG_GG
Cuts at: 0 564 960
Size: 564 396
PflMI CCAn_nnn'nTGG
Cuts at: 0 797 960
Size: 797 163
PfoI T'CCnGG_A
Cuts at: 0 920 960
Size: 920 40
PpuMI rG'GwC_Cy
Cuts at: 0 912 960
Size: 912 48
PspOMI G'GGCC_C
Cuts at: 0 567 960
Size: 567 393
PstI C_TGCA'G
Cuts at: 0 694 960
Size: 694 266
SapI GCTCTTCn'nnn_
Cuts at: 0 262 960
Size: 262 698
SfcI C'TryA_G
Cuts at: 0 369 690 960
Size: 369 321 270
Fragments arranged by size:
369 321 270
StuI AGG'CCT
Cuts at: 0 466 960
Size: 466 494
StyI C'CwwG_G
Cuts at: 0 116 230 578 811 954 960
Size: 116 114 348 233 143 6
Fragments arranged by size:
348 233 143 116 114 6
TatI w'GTAC_w
Cuts at: 0 884 960
Size: 884 76
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 237 960
Size: 237 723
XmnI GAAnn'nnTTC
Cuts at: 0 16 960
Size: 16 944
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AlwNI ApaI BanI
BanII BbsI BfrBI BglI Bme1580I BmrI BpmI BsaHI
BsaWI BsaXI BseRI BseYI Bsp1286I BspEI BspHI BspMI
BsrBI Bsu36I BtgI BtsI EarI Eco57MI EcoNI EcoO109I
FalI Hin4I HindIII KpnI MmeI MslI MspA1I NcoI
NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI
SapI SfcI StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AatII AfeI AflII AflIII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglII
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI ZraI