Restriction map for
pFN21AE6680
(Linear) (Six-base) MAPSORT of: KIEE6680.seq Check: 113 from: 1 to: 1014
KIEE6680.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AatII G_ACGT'C
Cuts at: 0 32 227 917 1014
Size: 32 195 690 97
Fragments arranged by size:
690 195 97 32
AccI GT'mk_AC
Cuts at: 0 773 1014
Size: 773 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1014
Size: 1006 8
AflIII A'CryG_T
Cuts at: 0 251 274 1014
Size: 251 23 740
Fragments arranged by size:
740 251 23
ApaI G_GGCC'C
Cuts at: 0 729 1014
Size: 729 285
AvaI C'yCGr_G
Cuts at: 0 976 1014
Size: 976 38
BamHI G'GATC_C
Cuts at: 0 958 1014
Size: 958 56
BanI G'GyrC_C
Cuts at: 0 12 942 1014
Size: 12 930 72
Fragments arranged by size:
930 72 12
BanII G_rGCy'C
Cuts at: 0 729 742 814 1014
Size: 729 13 72 200
Fragments arranged by size:
729 200 72 13
BbeI G_GCGC'C
Cuts at: 0 16 946 1014
Size: 16 930 68
Fragments arranged by size:
930 68 16
BclI T'GATC_A
Cuts at: 0 204 1014
Size: 204 810
Bme1580I G_kGCm'C
Cuts at: 0 729 1014
Size: 729 285
BmgBI CAC'GTC
Cuts at: 0 972 1014
Size: 972 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 902 1014
Size: 902 112
BsaAI yAC'GTr
Cuts at: 0 275 1014
Size: 275 739
BsaBI GATnn'nnATC
Cuts at: 0 758 1014
Size: 758 256
BsaHI Gr'CG_yC
Cuts at: 0 13 29 224 914 943 1014
Size: 13 16 195 690 29 71
Fragments arranged by size:
690 195 71 29 16 13
BsaWI w'CCGG_w
Cuts at: 0 321 1014
Size: 321 693
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 892 922 1014
Size: 214 30 648 30 92
Fragments arranged by size:
648 214 92 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 1014
Size: 73 941
BseYI C'CCAG_C
Cuts at: 0 95 874 1014
Size: 95 779 140
Fragments arranged by size:
779 140 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 1014
Size: 99 915
BsiHKAI G_wGCw'C
Cuts at: 0 977 1014
Size: 977 37
BsmI GAATG_Cn'
Cuts at: 0 847 1014
Size: 847 167
Bsp1286I G_dGCh'C
Cuts at: 0 729 742 814 977 1014
Size: 729 13 72 163 37
Fragments arranged by size:
729 163 72 37 13
BsrDI GCAATG_nn'
Cuts at: 0 176 1014
Size: 176 838
BsrFI r'CCGG_y
Cuts at: 0 487 945 1014
Size: 487 458 69
Fragments arranged by size:
487 458 69
BssSI C'ACGA_G
Cuts at: 0 283 1014
Size: 283 731
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 315 519 1014
Size: 240 75 204 495
Fragments arranged by size:
495 240 204 75
BstYI r'GATC_y
Cuts at: 0 367 958 1014
Size: 367 591 56
Fragments arranged by size:
591 367 56
BtgI C'CryG_G
Cuts at: 0 75 530 743 851 1014
Size: 75 455 213 108 163
Fragments arranged by size:
455 213 163 108 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 144 745 1014
Size: 144 601 269
Fragments arranged by size:
601 269 144
BtsI GCAGTG_nn'
Cuts at: 0 513 1014
Size: 513 501
ClaI AT'CG_AT
Cuts at: 0 380 1014
Size: 380 634
DrdI GACnn_nn'nnGTC
Cuts at: 0 28 541 667 1014
Size: 28 513 126 347
Fragments arranged by size:
513 347 126 28
EaeI y'GGCC_r
Cuts at: 0 566 884 1014
Size: 566 318 130
Fragments arranged by size:
566 318 130
EarI CTCTTCn'nnn_
Cuts at: 0 141 802 1014
Size: 141 661 212
Fragments arranged by size:
661 212 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1014
Size: 1006 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 637 1014
Size: 637 377
EcoO109I rG'GnC_Cy
Cuts at: 0 725 1014
Size: 725 289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 282 1014
Size: 282 732
FspI TGC'GCA
Cuts at: 0 148 1014
Size: 148 866
HaeII r_GCGC'y
Cuts at: 0 16 946 1014
Size: 16 930 68
Fragments arranged by size:
930 68 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 189 214 221 246 1014
Size: 189 25 7 25 768
Fragments arranged by size:
768 189 25 25 7
HincII GTy'rAC
Cuts at: 0 33 92 546 1014
Size: 33 59 454 468
Fragments arranged by size:
468 454 59 33
KasI G'GCGC_C
Cuts at: 0 12 942 1014
Size: 12 930 72
Fragments arranged by size:
930 72 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 378 398 1014
Size: 257 121 20 616
Fragments arranged by size:
616 257 121 20
MscI TGG'CCA
Cuts at: 0 886 1014
Size: 886 128
MslI CAynn'nnrTG
Cuts at: 0 170 313 586 1014
Size: 170 143 273 428
Fragments arranged by size:
428 273 170 143
MspA1I CmG'CkG
Cuts at: 0 77 99 1014
Size: 77 22 915
Fragments arranged by size:
915 77 22
NaeI GCC'GGC
Cuts at: 0 489 1014
Size: 489 525
NarI GG'CG_CC
Cuts at: 0 13 943 1014
Size: 13 930 71
Fragments arranged by size:
930 71 13
NcoI C'CATG_G
Cuts at: 0 530 743 851 1014
Size: 530 213 108 163
Fragments arranged by size:
530 213 163 108
NgoMIV G'CCGG_C
Cuts at: 0 487 1014
Size: 487 527
NspI r_CATG'y
Cuts at: 0 255 1014
Size: 255 759
PciI A'CATG_T
Cuts at: 0 251 1014
Size: 251 763
PmlI CAC'GTG
Cuts at: 0 275 1014
Size: 275 739
PspOMI G'GGCC_C
Cuts at: 0 725 1014
Size: 725 289
PspXI vC'TCGA_Gb
Cuts at: 0 976 1014
Size: 976 38
PvuII CAG'CTG
Cuts at: 0 99 1014
Size: 99 915
SacII CC_GC'GG
Cuts at: 0 78 1014
Size: 78 936
SapI GCTCTTCn'nnn_
Cuts at: 0 802 1014
Size: 802 212
SexAI A'CCwGG_T
Cuts at: 0 239 476 1014
Size: 239 237 538
Fragments arranged by size:
538 239 237
SfcI C'TryA_G
Cuts at: 0 417 627 1014
Size: 417 210 387
Fragments arranged by size:
417 387 210
SfoI GGC'GCC
Cuts at: 0 14 944 1014
Size: 14 930 70
Fragments arranged by size:
930 70 14
SgrAI Cr'CCGG_yG
Cuts at: 0 945 1014
Size: 945 69
SmlI C'TyrA_G
Cuts at: 0 976 1014
Size: 976 38
StyI C'CwwG_G
Cuts at: 0 530 743 851 1014
Size: 530 213 108 163
Fragments arranged by size:
530 213 163 108
TatI w'GTAC_w
Cuts at: 0 677 1014
Size: 677 337
XhoI C'TCGA_G
Cuts at: 0 976 1014
Size: 976 38
XmnI GAAnn'nnTTC
Cuts at: 0 694 1014
Size: 694 320
ZraI GAC'GTC
Cuts at: 0 30 225 915 1014
Size: 30 195 690 99
Fragments arranged by size:
690 195 99 30
Enzymes that do cut:
AatII AccI AcuI AflIII ApaI AvaI BamHI BanI
BanII BbeI BclI Bme1580I BmgBI BmrI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI
BtsI ClaI DrdI EaeI EarI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmlI PspOMI PspXI PvuII SacII SapI SexAI SfcI
SfoI SgrAI SmlI StyI TatI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I CspCI DraI
DraIII EagI EciI EcoICRI EcoRI EcoRV FseI FspAI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI RsrII SacI
SalI SanDI SbfI ScaI SfiI SgfI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XmaI