Restriction map for
pFN21AE6676
(Linear) (Six-base) MAPSORT of: KIEE6676.seq Check: 9610 from: 1 to: 858
KIEE6676.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AflIII A'CryG_T
Cuts at: 0 205 596 629 858
Size: 205 391 33 229
Fragments arranged by size:
391 229 205 33
AlwNI CAG_nnn'CTG
Cuts at: 0 585 840 858
Size: 585 255 18
Fragments arranged by size:
585 255 18
AseI AT'TA_AT
Cuts at: 0 734 858
Size: 734 124
BamHI G'GATC_C
Cuts at: 0 65 858
Size: 65 793
BanII G_rGCy'C
Cuts at: 0 252 858
Size: 252 606
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 177 763 858
Size: 90 87 586 95
Fragments arranged by size:
586 95 90 87
BfrBI ATG'CAT
Cuts at: 0 55 858
Size: 55 803
BglI GCCn_nnn'nGGC
Cuts at: 0 283 858
Size: 283 575
Bme1580I G_kGCm'C
Cuts at: 0 25 858
Size: 25 833
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 858
Size: 663 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 858
Size: 517 341
BsaHI Gr'CG_yC
Cuts at: 0 110 858
Size: 110 748
BsaWI w'CCGG_w
Cuts at: 0 383 777 858
Size: 383 394 81
Fragments arranged by size:
394 383 81
BseYI C'CCAG_C
Cuts at: 0 255 858
Size: 255 603
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 858
Size: 230 628
BsiHKAI G_wGCw'C
Cuts at: 0 232 508 858
Size: 232 276 350
Fragments arranged by size:
350 276 232
BsmI GAATG_Cn'
Cuts at: 0 153 858
Size: 153 705
Bsp1286I G_dGCh'C
Cuts at: 0 25 232 252 508 858
Size: 25 207 20 256 350
Fragments arranged by size:
350 256 207 25 20
BspEI T'CCGG_A
Cuts at: 0 383 858
Size: 383 475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 526 858
Size: 526 332
BsrBI CCG'CTC
Cuts at: 0 71 858
Size: 71 787
BstAPI GCAn_nnn'nTGC
Cuts at: 0 840 858
Size: 840 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 858
Size: 81 777
BstYI r'GATC_y
Cuts at: 0 65 858
Size: 65 793
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 29 858
Size: 29 829
BtsI GCAGTG_nn'
Cuts at: 0 603 858
Size: 603 255
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 270 305 858
Size: 270 35 553
Fragments arranged by size:
553 270 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 664 858
Size: 664 194
EaeI y'GGCC_r
Cuts at: 0 170 200 291 465 858
Size: 170 30 91 174 393
Fragments arranged by size:
393 174 170 91 30
EarI CTCTTCn'nnn_
Cuts at: 0 144 844 858
Size: 144 700 14
Fragments arranged by size:
700 144 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 858
Size: 517 341
EcoO109I rG'GnC_Cy
Cuts at: 0 192 261 858
Size: 192 69 597
Fragments arranged by size:
597 192 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 167 199 858
Size: 40 32 95 32 659
Fragments arranged by size:
659 95 40 32 32
MluI A'CGCG_T
Cuts at: 0 205 858
Size: 205 653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 687 858
Size: 395 292 171
Fragments arranged by size:
395 292 171
MscI TGG'CCA
Cuts at: 0 172 293 467 858
Size: 172 121 174 391
Fragments arranged by size:
391 174 172 121
MslI CAynn'nnrTG
Cuts at: 0 79 858
Size: 79 779
MspA1I CmG'CkG
Cuts at: 0 132 837 858
Size: 132 705 21
Fragments arranged by size:
705 132 21
NsiI A_TGCA'T
Cuts at: 0 57 858
Size: 57 801
NspI r_CATG'y
Cuts at: 0 277 600 633 858
Size: 277 323 33 225
Fragments arranged by size:
323 277 225 33
PciI A'CATG_T
Cuts at: 0 596 629 858
Size: 596 33 229
Fragments arranged by size:
596 229 33
PfoI T'CCnGG_A
Cuts at: 0 494 526 858
Size: 494 32 332
Fragments arranged by size:
494 332 32
PpuMI rG'GwC_Cy
Cuts at: 0 192 261 858
Size: 192 69 597
Fragments arranged by size:
597 192 69
PvuII CAG'CTG
Cuts at: 0 132 837 858
Size: 132 705 21
Fragments arranged by size:
705 132 21
SanDI GG'GwC_CC
Cuts at: 0 261 858
Size: 261 597
SphI G_CATG'C
Cuts at: 0 277 858
Size: 277 581
StuI AGG'CCT
Cuts at: 0 242 858
Size: 242 616
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 627 858
Size: 627 231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 118 150 858
Size: 118 32 708
Fragments arranged by size:
708 118 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 858
Size: 492 366
XmnI GAAnn'nnTTC
Cuts at: 0 184 858
Size: 184 674
Enzymes that do cut:
AflIII AlwNI AseI BamHI BanII BbsI BfrBI BglI
Bme1580I BmrI BpmI BsaHI BsaWI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrBI BstAPI BstXI BstYI
BtgZI BtsI CspCI DrdI EaeI EarI Eco57MI EcoO109I
Hin4I MluI MmeI MscI MslI MspA1I NsiI NspI
PciI PfoI PpuMI PvuII SanDI SphI StuI TaqII
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI
BcgI BciVI BclI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsiEI
BsiWI BsmBI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgI ClaI DraI DraIII
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StyI SwaI TatI Tth111I XbaI XhoI XmaI
ZraI