Restriction map for
pFN21AE6675
(Linear) (Six-base) MAPSORT of: KIEE6675.seq Check: 4763 from: 1 to: 936
KIEE6675.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AatII G_ACGT'C
Cuts at: 0 827 936
Size: 827 109
AclI AA'CG_TT
Cuts at: 0 113 936
Size: 113 823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 928 936
Size: 678 250 8
Fragments arranged by size:
678 250 8
AflIII A'CryG_T
Cuts at: 0 508 936
Size: 508 428
ApaLI G'TGCA_C
Cuts at: 0 331 936
Size: 331 605
ApoI r'AATT_y
Cuts at: 0 709 936
Size: 709 227
AvaI C'yCGr_G
Cuts at: 0 255 574 757 936
Size: 255 319 183 179
Fragments arranged by size:
319 255 183 179
BamHI G'GATC_C
Cuts at: 0 73 936
Size: 73 863
BanI G'GyrC_C
Cuts at: 0 580 936
Size: 580 356
BanII G_rGCy'C
Cuts at: 0 55 936
Size: 55 881
BbsI GAAGACnn'nnnn_
Cuts at: 0 827 916 936
Size: 827 89 20
Fragments arranged by size:
827 89 20
BciVI GTATCCnnnnn_n'
Cuts at: 0 734 936
Size: 734 202
Bme1580I G_kGCm'C
Cuts at: 0 335 583 936
Size: 335 248 353
Fragments arranged by size:
353 335 248
BmrI ACTGGGnnnn_n'
Cuts at: 0 354 936
Size: 354 582
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 46 936
Size: 46 890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 936
Size: 345 591
BsaI GGTCTCn'nnnn_
Cuts at: 0 279 936
Size: 279 657
BsaBI GATnn'nnATC
Cuts at: 0 805 936
Size: 805 131
BsaHI Gr'CG_yC
Cuts at: 0 824 936
Size: 824 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 301 331 936
Size: 301 30 605
Fragments arranged by size:
605 301 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 936
Size: 205 731
BseYI C'CCAG_C
Cuts at: 0 36 936
Size: 36 900
BsiEI CG_ry'CG
Cuts at: 0 21 936
Size: 21 915
BsiHKAI G_wGCw'C
Cuts at: 0 55 335 604 936
Size: 55 280 269 332
Fragments arranged by size:
332 280 269 55
BsmI GAATG_Cn'
Cuts at: 0 231 936
Size: 231 705
Bsp1286I G_dGCh'C
Cuts at: 0 55 335 583 604 936
Size: 55 280 248 21 332
Fragments arranged by size:
332 280 248 55 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 551 936
Size: 146 405 385
Fragments arranged by size:
405 385 146
BsrFI r'CCGG_y
Cuts at: 0 16 936
Size: 16 920
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 936
Size: 396 540
BstYI r'GATC_y
Cuts at: 0 73 936
Size: 73 863
BtsI GCAGTG_nn'
Cuts at: 0 251 936
Size: 251 685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 848 883 936
Size: 848 35 53
Fragments arranged by size:
848 53 35
EaeI y'GGCC_r
Cuts at: 0 18 662 936
Size: 18 644 274
Fragments arranged by size:
644 274 18
EagI C'GGCC_G
Cuts at: 0 18 936
Size: 18 918
EarI CTCTTCn'nnn_
Cuts at: 0 223 936
Size: 223 713
EcoICRI GAG'CTC
Cuts at: 0 53 936
Size: 53 883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 678 928 936
Size: 345 333 250 8
Fragments arranged by size:
345 333 250 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 324 936
Size: 324 612
EcoO109I rG'GnC_Cy
Cuts at: 0 179 762 936
Size: 179 583 174
Fragments arranged by size:
583 179 174
EcoRV GAT'ATC
Cuts at: 0 422 936
Size: 422 514
FspI TGC'GCA
Cuts at: 0 236 936
Size: 236 700
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 936
Size: 301 32 603
Fragments arranged by size:
603 301 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 936
Size: 605 331
MslI CAynn'nnrTG
Cuts at: 0 127 394 437 844 936
Size: 127 267 43 407 92
Fragments arranged by size:
407 267 127 92 43
NspI r_CATG'y
Cuts at: 0 512 936
Size: 512 424
PasI CC'CwG_GG
Cuts at: 0 150 415 936
Size: 150 265 521
Fragments arranged by size:
521 265 150
PciI A'CATG_T
Cuts at: 0 508 936
Size: 508 428
PpuMI rG'GwC_Cy
Cuts at: 0 179 762 936
Size: 179 583 174
Fragments arranged by size:
583 179 174
RsrII CG'GwC_CG
Cuts at: 0 14 936
Size: 14 922
SacI G_AGCT'C
Cuts at: 0 55 936
Size: 55 881
ScaI AGT'ACT
Cuts at: 0 364 936
Size: 364 572
SspI AAT'ATT
Cuts at: 0 189 936
Size: 189 747
StuI AGG'CCT
Cuts at: 0 26 936
Size: 26 910
StyI C'CwwG_G
Cuts at: 0 704 728 936
Size: 704 24 208
Fragments arranged by size:
704 208 24
TatI w'GTAC_w
Cuts at: 0 362 936
Size: 362 574
Tth111I GACn'n_nGTC
Cuts at: 0 390 936
Size: 390 546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 936
Size: 621 315
ZraI GAC'GTC
Cuts at: 0 825 936
Size: 825 111
Enzymes that do cut:
AatII AclI AcuI AflIII ApaLI ApoI AvaI BamHI
BanI BanII BbsI BciVI Bme1580I BmrI BplI BpmI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BstXI BstYI BtsI CspCI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FspI Hin4I MmeI MslI NspI PasI PciI PpuMI
RsrII SacI ScaI SspI StuI StyI TatI Tth111I
XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BclI BfrBI BglI BglII
BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaWI BsgI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EciI EcoRI FalI FseI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SwaI TaqII TstI XbaI XhoI
XmaI XmnI