(Linear) (Six-base) MAPSORT of: KIEE6670.seq  Check: 74  from: 1  to: 888



KIEE6670.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:35  ..

Acc65I G'GTAC_C

Cuts at:      0     811     888 
   Size:        811      77
 
AflIII A'CryG_T

Cuts at:      0     454     888 
   Size:        454     434
 
ApoI r'AATT_y

Cuts at:      0     364     629     888 
   Size:        364     265     259

  Fragments arranged by size: 

                 364     265     259
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     803     836     888 
   Size:        803      33      52

  Fragments arranged by size: 

                 803      52      33
 
BanI G'GyrC_C

Cuts at:      0     811     888 
   Size:        811      77
 
BbvCI CC'TCA_GC

Cuts at:      0     650     888 
   Size:        650     238
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     613     888 
   Size:        613     275
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     888 
   Size:        128     760
 
Bpu10I CC'TnA_GC

Cuts at:      0     623     650     888 
   Size:        623      27     238

  Fragments arranged by size: 

                 623     238      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     888 
   Size:        185     703
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     428     888 
   Size:        122     306     460

  Fragments arranged by size: 

                 460     306     122
 
BsiHKAI G_wGCw'C

Cuts at:      0     599     888 
   Size:        599     289
 
Bsp1286I G_dGCh'C

Cuts at:      0     599     888 
   Size:        599     289
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     223     888 
   Size:        223     665
 
EaeI y'GGCC_r

Cuts at:      0       2     888 
   Size:          2     886
 
EarI CTCTTCn'nnn_

Cuts at:      0     647     888 
   Size:        647     241
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     888 
   Size:        128     760
 
EcoO109I rG'GnC_Cy

Cuts at:      0     187     888 
   Size:        187     701
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     139     171     469     501     667     699     888 
   Size:        139      32     298      32     166      32     189

  Fragments arranged by size: 

                 298     189     166     139      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0     815     888 
   Size:        815      73
 
MscI TGG'CCA

Cuts at:      0       4     888 
   Size:          4     884
 
MslI CAynn'nnrTG

Cuts at:      0     251     888 
   Size:        251     637
 
NspI r_CATG'y

Cuts at:      0     458     888 
   Size:        458     430
 
PciI A'CATG_T

Cuts at:      0     454     888 
   Size:        454     434
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     685     888 
   Size:        685     203
 
PpuMI rG'GwC_Cy

Cuts at:      0     187     888 
   Size:        187     701
 
PstI C_TGCA'G

Cuts at:      0     102     888 
   Size:        102     786
 
SanDI GG'GwC_CC

Cuts at:      0     187     888 
   Size:        187     701
 
SapI GCTCTTCn'nnn_

Cuts at:      0     647     888 
   Size:        647     241
 
SfcI C'TryA_G

Cuts at:      0      98     888 
   Size:         98     790
 
SmlI C'TyrA_G

Cuts at:      0     200     888 
   Size:        200     688
 
StyI C'CwwG_G

Cuts at:      0     774     888 
   Size:        774     114
 

 Enzymes that do cut:

   Acc65I   AflIII     ApoI     BaeI     BanI    BbvCI     BplI     BpmI
   Bpu10I    BpuEI     BsgI  BsiHKAI Bsp1286I    BstXI     EaeI     EarI
  Eco57MI EcoO109I    Hin4I     KpnI     MscI     MslI     NspI     PciI
    PflMI    PpuMI     PstI    SanDI     SapI     SfcI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI