(Linear) (Six-base) MAPSORT of: KIEE6664.seq  Check: 983  from: 1  to: 849



KIEE6664.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:35  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     829     849 
   Size:        829      20
 
AflIII A'CryG_T

Cuts at:      0     261     849 
   Size:        261     588
 
AleI CACnn'nnGTG

Cuts at:      0      25     849 
   Size:         25     824
 
ApoI r'AATT_y

Cuts at:      0      71     626     849 
   Size:         71     555     223

  Fragments arranged by size: 

                 555     223      71
 
AvaI C'yCGr_G

Cuts at:      0     536     849 
   Size:        536     313
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     624     657     849 
   Size:        624      33     192

  Fragments arranged by size: 

                 624     192      33
 
BanI G'GyrC_C

Cuts at:      0      10     485     849 
   Size:         10     475     364

  Fragments arranged by size: 

                 475     364      10
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      96     849 
   Size:         96     753
 
BclI T'GATC_A

Cuts at:      0     330     849 
   Size:        330     519
 
BlpI GC'TnA_GC

Cuts at:      0     422     849 
   Size:        422     427
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     816     849 
   Size:        816      33
 
Bpu10I CC'TnA_GC

Cuts at:      0     614     849 
   Size:        614     235
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312     849 
   Size:        312     537
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     351     849 
   Size:        351     498
 
BsaWI w'CCGG_w

Cuts at:      0     482     849 
   Size:        482     367
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     313     413     849 
   Size:        313     100     436

  Fragments arranged by size: 

                 436     313     100
 
BsiHKAI G_wGCw'C

Cuts at:      0     680     849 
   Size:        680     169
 
Bsp1286I G_dGCh'C

Cuts at:      0     680     849 
   Size:        680     169
 
BsrGI T'GTAC_A

Cuts at:      0     256     849 
   Size:        256     593
 
BssSI C'ACGA_G

Cuts at:      0     679     849 
   Size:        679     170
 
BtgI C'CryG_G

Cuts at:      0     647     849 
   Size:        647     202
 
EarI CTCTTCn'nnn_

Cuts at:      0     242     849 
   Size:        242     607
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     586     849 
   Size:        586     263
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     829     849 
   Size:        829      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     770     849 
   Size:        770      79
 
EcoO109I rG'GnC_Cy

Cuts at:      0     520     849 
   Size:        520     329
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     404     849 
   Size:        404     445
 
HincII GTy'rAC

Cuts at:      0     216     849 
   Size:        216     633
 
HindIII A'AGCT_T

Cuts at:      0     546     744     849 
   Size:        546     198     105

  Fragments arranged by size: 

                 546     198     105
 
MslI CAynn'nnrTG

Cuts at:      0      25     199     389     849 
   Size:         25     174     190     460

  Fragments arranged by size: 

                 460     190     174      25
 
NcoI C'CATG_G

Cuts at:      0     647     849 
   Size:        647     202
 
NspI r_CATG'y

Cuts at:      0     265     849 
   Size:        265     584
 
PciI A'CATG_T

Cuts at:      0     261     849 
   Size:        261     588
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     785     849 
   Size:        785      64
 
PpuMI rG'GwC_Cy

Cuts at:      0     520     849 
   Size:        520     329
 
PshAI GACnn'nnGTC

Cuts at:      0     363     505     849 
   Size:        363     142     344

  Fragments arranged by size: 

                 363     344     142
 
SmaI CCC'GGG

Cuts at:      0     538     849 
   Size:        538     311
 
SmlI C'TyrA_G

Cuts at:      0     291     849 
   Size:        291     558
 
StuI AGG'CCT

Cuts at:      0      56     849 
   Size:         56     793
 
StyI C'CwwG_G

Cuts at:      0     647     849 
   Size:        647     202
 
TatI w'GTAC_w

Cuts at:      0     256     849 
   Size:        256     593
 
Tth111I GACn'n_nGTC

Cuts at:      0     703     849 
   Size:        703     146
 
XmaI C'CCGG_G

Cuts at:      0     536     849 
   Size:        536     313
 
XmnI GAAnn'nnTTC

Cuts at:      0     384     849 
   Size:        384     465
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     AvaI     BaeI     BanI    BciVI
     BclI     BlpI     BmrI   Bpu10I    BpuEI     BsaI    BsaWI    BseRI
  BsiHKAI Bsp1286I    BsrGI    BssSI     BtgI     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FalI   HincII  HindIII     MslI     NcoI     NspI
     PciI    PflMI    PpuMI    PshAI     SmaI     SmlI     StuI     StyI
     TatI  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI     BplI     BpmI
    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI     ZraI