Restriction map for
pFN21AE6662
(Linear) (Six-base) MAPSORT of: KIEE6662.seq Check: 4587 from: 1 to: 828
KIEE6662.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AccI GT'mk_AC
Cuts at: 0 782 828
Size: 782 46
AlwNI CAG_nnn'CTG
Cuts at: 0 186 828
Size: 186 642
AvaI C'yCGr_G
Cuts at: 0 464 828
Size: 464 364
BanI G'GyrC_C
Cuts at: 0 469 828
Size: 469 359
BbeI G_GCGC'C
Cuts at: 0 473 828
Size: 473 355
BbvCI CC'TCA_GC
Cuts at: 0 58 828
Size: 58 770
BlpI GC'TnA_GC
Cuts at: 0 550 828
Size: 550 278
Bme1580I G_kGCm'C
Cuts at: 0 793 828
Size: 793 35
BmgBI CAC'GTC
Cuts at: 0 89 828
Size: 89 739
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 490 828
Size: 490 338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 696 828
Size: 49 647 132
Fragments arranged by size:
647 132 49
Bpu10I CC'TnA_GC
Cuts at: 0 58 828
Size: 58 770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 828
Size: 625 203
BsaI GGTCTCn'nnnn_
Cuts at: 0 403 828
Size: 403 425
BsaAI yAC'GTr
Cuts at: 0 786 828
Size: 786 42
BsaHI Gr'CG_yC
Cuts at: 0 5 37 470 828
Size: 5 32 433 358
Fragments arranged by size:
433 358 32 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 828
Size: 22 30 776
Fragments arranged by size:
776 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 828
Size: 283 545
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 828
Size: 219 609
BsmBI CGTCTCn'nnnn_
Cuts at: 0 95 828
Size: 95 733
Bsp1286I G_dGCh'C
Cuts at: 0 793 828
Size: 793 35
BsrDI GCAATG_nn'
Cuts at: 0 705 828
Size: 705 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 159 828
Size: 159 669
BtsI GCAGTG_nn'
Cuts at: 0 51 828
Size: 51 777
DraIII CAC_nnn'GTG
Cuts at: 0 242 828
Size: 242 586
EaeI y'GGCC_r
Cuts at: 0 452 730 828
Size: 452 278 98
Fragments arranged by size:
452 278 98
EarI CTCTTCn'nnn_
Cuts at: 0 139 401 481 828
Size: 139 262 80 347
Fragments arranged by size:
347 262 139 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 696 828
Size: 49 647 132
Fragments arranged by size:
647 132 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 828
Size: 190 638
EcoO109I rG'GnC_Cy
Cuts at: 0 54 183 315 671 828
Size: 54 129 132 356 157
Fragments arranged by size:
356 157 132 129 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 321 828
Size: 321 507
FspI TGC'GCA
Cuts at: 0 718 828
Size: 718 110
HaeII r_GCGC'y
Cuts at: 0 473 828
Size: 473 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 828
Size: 22 32 774
Fragments arranged by size:
774 32 22
KasI G'GCGC_C
Cuts at: 0 469 828
Size: 469 359
MscI TGG'CCA
Cuts at: 0 454 828
Size: 454 374
MslI CAynn'nnrTG
Cuts at: 0 685 828
Size: 685 143
NarI GG'CG_CC
Cuts at: 0 470 828
Size: 470 358
PfoI T'CCnGG_A
Cuts at: 0 673 828
Size: 673 155
PpuMI rG'GwC_Cy
Cuts at: 0 315 671 828
Size: 315 356 157
Fragments arranged by size:
356 315 157
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 760 792 828
Size: 760 32 36
Fragments arranged by size:
760 36 32
PstI C_TGCA'G
Cuts at: 0 350 828
Size: 350 478
RsrII CG'GwC_CG
Cuts at: 0 66 828
Size: 66 762
SanDI GG'GwC_CC
Cuts at: 0 315 828
Size: 315 513
SfcI C'TryA_G
Cuts at: 0 222 346 828
Size: 222 124 482
Fragments arranged by size:
482 222 124
SfoI GGC'GCC
Cuts at: 0 471 828
Size: 471 357
SmlI C'TyrA_G
Cuts at: 0 604 828
Size: 604 224
StyI C'CwwG_G
Cuts at: 0 150 647 828
Size: 150 497 181
Fragments arranged by size:
497 181 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 514 828
Size: 514 314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 270 828
Size: 270 558
Enzymes that do cut:
AccI AlwNI AvaI BanI BbeI BbvCI BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BsgI BsmBI Bsp1286I BsrDI BtgZI BtsI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I KasI MscI MslI NarI PfoI PpuMI
PsrI PstI RsrII SanDI SfcI SfoI SmlI StyI
TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BmrI BmtI
BsaBI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI