Restriction map for
pFN21AE6661
(Linear) (Six-base) MAPSORT of: KIEE6661.seq Check: 4749 from: 1 to: 822
KIEE6661.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 65 822
Size: 65 757
Acc65I G'GTAC_C
Cuts at: 0 487 822
Size: 487 335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 686 822
Size: 673 13 136
Fragments arranged by size:
673 136 13
ApaI G_GGCC'C
Cuts at: 0 45 822
Size: 45 777
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 508 541 822
Size: 508 33 281
Fragments arranged by size:
508 281 33
BanI G'GyrC_C
Cuts at: 0 4 487 726 822
Size: 4 483 239 96
Fragments arranged by size:
483 239 96 4
BanII G_rGCy'C
Cuts at: 0 45 822
Size: 45 777
BciVI GTATCCnnnnn_n'
Cuts at: 0 148 822
Size: 148 674
BclI T'GATC_A
Cuts at: 0 374 822
Size: 374 448
BglI GCCn_nnn'nGGC
Cuts at: 0 735 822
Size: 735 87
Bme1580I G_kGCm'C
Cuts at: 0 7 45 106 537 822
Size: 7 38 61 431 285
Fragments arranged by size:
431 285 61 38 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 90 447 822
Size: 90 357 375
Fragments arranged by size:
375 357 90
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 822
Size: 136 686
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 344 822
Size: 37 307 478
Fragments arranged by size:
478 307 37
BsmI GAATG_Cn'
Cuts at: 0 217 698 822
Size: 217 481 124
Fragments arranged by size:
481 217 124
Bsp1286I G_dGCh'C
Cuts at: 0 7 45 106 537 822
Size: 7 38 61 431 285
Fragments arranged by size:
431 285 61 38 7
BspHI T'CATG_A
Cuts at: 0 129 822
Size: 129 693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 80 822
Size: 65 15 742
Fragments arranged by size:
742 65 15
BsrDI GCAATG_nn'
Cuts at: 0 377 822
Size: 377 445
BssSI C'ACGA_G
Cuts at: 0 718 822
Size: 718 104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 578 613 822
Size: 578 35 209
Fragments arranged by size:
578 209 35
EaeI y'GGCC_r
Cuts at: 0 20 822
Size: 20 802
EarI CTCTTCn'nnn_
Cuts at: 0 309 692 822
Size: 309 383 130
Fragments arranged by size:
383 309 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 686 822
Size: 673 13 136
Fragments arranged by size:
673 136 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 583 676 703 822
Size: 583 93 27 119
Fragments arranged by size:
583 119 93 27
HaeII r_GCGC'y
Cuts at: 0 31 822
Size: 31 791
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 761 793 822
Size: 761 32 29
Fragments arranged by size:
761 32 29
HincII GTy'rAC
Cuts at: 0 330 635 822
Size: 330 305 187
Fragments arranged by size:
330 305 187
KpnI G_GTAC'C
Cuts at: 0 491 822
Size: 491 331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 822
Size: 494 328
MspA1I CmG'CkG
Cuts at: 0 363 522 630 732 822
Size: 363 159 108 102 90
Fragments arranged by size:
363 159 108 102 90
PspOMI G'GGCC_C
Cuts at: 0 41 822
Size: 41 781
PstI C_TGCA'G
Cuts at: 0 62 822
Size: 62 760
PvuII CAG'CTG
Cuts at: 0 363 522 630 822
Size: 363 159 108 192
Fragments arranged by size:
363 192 159 108
SapI GCTCTTCn'nnn_
Cuts at: 0 692 822
Size: 692 130
SfcI C'TryA_G
Cuts at: 0 58 822
Size: 58 764
StyI C'CwwG_G
Cuts at: 0 23 348 416 822
Size: 23 325 68 406
Fragments arranged by size:
406 325 68 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 714 741 822
Size: 714 27 81
Fragments arranged by size:
714 81 27
XbaI T'CTAG_A
Cuts at: 0 498 822
Size: 498 324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 822
Size: 30 792
XmnI GAAnn'nnTTC
Cuts at: 0 686 698 816 822
Size: 686 12 118 6
Fragments arranged by size:
686 118 12 6
Enzymes that do cut:
AarI Acc65I AcuI ApaI BaeI BanI BanII BciVI
BclI BglI Bme1580I BmrI BseRI BsgI BsmI Bsp1286I
BspHI BspMI BsrDI BssSI CspCI EaeI EarI Eco57MI
FalI HaeII Hin4I HincII KpnI MmeI MspA1I PspOMI
PstI PvuII SapI SfcI StyI TaqII XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BamHI BbeI BbsI BbvCI BcgI
BfrBI BglII BlpI BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XhoI XmaI ZraI