(Linear) (Six-base) MAPSORT of: KIEE6658.seq  Check: 5541  from: 1  to: 765



KIEE6658.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:35  ..

AccI GT'mk_AC

Cuts at:      0      80     765 
   Size:         80     685
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     765 
   Size:        128     637
 
AlwNI CAG_nnn'CTG

Cuts at:      0      13     237     396     765 
   Size:         13     224     159     369

  Fragments arranged by size: 

                 369     224     159      13
 
ApaLI G'TGCA_C

Cuts at:      0     357     765 
   Size:        357     408
 
ApoI r'AATT_y

Cuts at:      0     327     765 
   Size:        327     438
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     758     765 
   Size:        758       7
 
BglII A'GATC_T

Cuts at:      0     405     765 
   Size:        405     360
 
Bme1580I G_kGCm'C

Cuts at:      0     361     765 
   Size:        361     404
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     480     765 
   Size:        480     285
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       5     556     591     765 
   Size:          5     551      35     174

  Fragments arranged by size: 

                 551     174      35       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     765 
   Size:         26     739
 
BsiHKAI G_wGCw'C

Cuts at:      0     361     765 
   Size:        361     404
 
BsmI GAATG_Cn'

Cuts at:      0     438     658     765 
   Size:        438     220     107

  Fragments arranged by size: 

                 438     220     107
 
Bsp1286I G_dGCh'C

Cuts at:      0     361     765 
   Size:        361     404
 
BsrDI GCAATG_nn'

Cuts at:      0     164     765 
   Size:        164     601
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      13     765 
   Size:         13     752
 
BstYI r'GATC_y

Cuts at:      0     405     765 
   Size:        405     360
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     199     234     765 
   Size:        199      35     531

  Fragments arranged by size: 

                 531     199      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     284     476     765 
   Size:        284     192     289

  Fragments arranged by size: 

                 289     284     192
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       5     128     556     591     765 
   Size:          5     123     428      35     174

  Fragments arranged by size: 

                 428     174     123      35       5
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     390     422     573     605     658     690     765 
   Size:        390      32     151      32      53      32      75

  Fragments arranged by size: 

                 390     151      75      53      32      32      32
 
HincII GTy'rAC

Cuts at:      0     164     489     765 
   Size:        164     325     276

  Fragments arranged by size: 

                 325     276     164
 
HindIII A'AGCT_T

Cuts at:      0     724     765 
   Size:        724      41
 
MfeI C'AATT_G

Cuts at:      0      91     505     765 
   Size:         91     414     260

  Fragments arranged by size: 

                 414     260      91
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     728     765 
   Size:        728      37
 
MspA1I CmG'CkG

Cuts at:      0     396     765 
   Size:        396     369
 
NdeI CA'TA_TG

Cuts at:      0      41     765 
   Size:         41     724
 
PstI C_TGCA'G

Cuts at:      0     746     765 
   Size:        746      19
 
PvuII CAG'CTG

Cuts at:      0     396     765 
   Size:        396     369
 
SfcI C'TryA_G

Cuts at:      0     742     765 
   Size:        742      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     461     765 
   Size:        461     304
 
TatI w'GTAC_w

Cuts at:      0      68     765 
   Size:         68     697
 
XbaI T'CTAG_A

Cuts at:      0     585     765 
   Size:        585     180
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI    ApaLI     ApoI     BbsI    BglII Bme1580I
     BplI     BpmI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrDI   BstAPI
    BstYI    CspCI     EarI  Eco57MI    Hin4I   HincII  HindIII     MfeI
     MmeI   MspA1I     NdeI     PstI    PvuII     SfcI    TaqII     TatI
     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MluI     MscI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI