Restriction map for
pFN21AE6655
(Linear) (Six-base) MAPSORT of: KIEE6655.seq Check: 9789 from: 1 to: 759
KIEE6655.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 321 463 759
Size: 321 142 296
Fragments arranged by size:
321 296 142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 454 691 759
Size: 199 255 237 68
Fragments arranged by size:
255 237 199 68
AflIII A'CryG_T
Cuts at: 0 167 759
Size: 167 592
AleI CACnn'nnGTG
Cuts at: 0 172 759
Size: 172 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 556 759
Size: 556 203
AlwNI CAG_nnn'CTG
Cuts at: 0 696 759
Size: 696 63
ApaI G_GGCC'C
Cuts at: 0 489 759
Size: 489 270
AvaI C'yCGr_G
Cuts at: 0 439 482 759
Size: 439 43 277
Fragments arranged by size:
439 277 43
BamHI G'GATC_C
Cuts at: 0 638 759
Size: 638 121
BanI G'GyrC_C
Cuts at: 0 254 759
Size: 254 505
BanII G_rGCy'C
Cuts at: 0 489 759
Size: 489 270
BbeI G_GCGC'C
Cuts at: 0 258 759
Size: 258 501
BglI GCCn_nnn'nGGC
Cuts at: 0 287 572 730 759
Size: 287 285 158 29
Fragments arranged by size:
287 285 158 29
BglII A'GATC_T
Cuts at: 0 338 759
Size: 338 421
Bme1580I G_kGCm'C
Cuts at: 0 489 759
Size: 489 270
BmgBI CAC'GTC
Cuts at: 0 170 759
Size: 170 589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 759
Size: 363 396
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 759
Size: 451 308
BsaHI Gr'CG_yC
Cuts at: 0 255 759
Size: 255 504
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 385 406 415 436 759
Size: 385 21 9 21 323
Fragments arranged by size:
385 323 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 340 520 759
Size: 121 219 180 239
Fragments arranged by size:
239 219 180 121
BsiEI CG_ry'CG
Cuts at: 0 311 759
Size: 311 448
BsiHKAI G_wGCw'C
Cuts at: 0 43 545 759
Size: 43 502 214
Fragments arranged by size:
502 214 43
Bsp1286I G_dGCh'C
Cuts at: 0 43 489 545 759
Size: 43 446 56 214
Fragments arranged by size:
446 214 56 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 321 463 635 759
Size: 321 142 172 124
Fragments arranged by size:
321 172 142 124
BsrBI CCG'CTC
Cuts at: 0 267 759
Size: 267 492
BsrDI GCAATG_nn'
Cuts at: 0 476 545 759
Size: 476 69 214
Fragments arranged by size:
476 214 69
BsrFI r'CCGG_y
Cuts at: 0 529 759
Size: 529 230
BssSI C'ACGA_G
Cuts at: 0 16 759
Size: 16 743
BstYI r'GATC_y
Cuts at: 0 338 638 759
Size: 338 300 121
Fragments arranged by size:
338 300 121
BtgI C'CryG_G
Cuts at: 0 555 632 725 759
Size: 555 77 93 34
Fragments arranged by size:
555 93 77 34
EaeI y'GGCC_r
Cuts at: 0 2 308 759
Size: 2 306 451
Fragments arranged by size:
451 306 2
EagI C'GGCC_G
Cuts at: 0 308 759
Size: 308 451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 363 454 691 759
Size: 199 164 91 237 68
Fragments arranged by size:
237 199 164 91 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 759
Size: 295 464
EcoO109I rG'GnC_Cy
Cuts at: 0 279 486 759
Size: 279 207 273
Fragments arranged by size:
279 273 207
FspI TGC'GCA
Cuts at: 0 247 759
Size: 247 512
FspAI rTGC'GCAy
Cuts at: 0 247 759
Size: 247 512
HaeII r_GCGC'y
Cuts at: 0 258 351 759
Size: 258 93 408
Fragments arranged by size:
408 258 93
KasI G'GCGC_C
Cuts at: 0 254 759
Size: 254 505
MscI TGG'CCA
Cuts at: 0 4 759
Size: 4 755
MslI CAynn'nnrTG
Cuts at: 0 172 759
Size: 172 587
NaeI GCC'GGC
Cuts at: 0 531 759
Size: 531 228
NarI GG'CG_CC
Cuts at: 0 255 759
Size: 255 504
NcoI C'CATG_G
Cuts at: 0 725 759
Size: 725 34
NgoMIV G'CCGG_C
Cuts at: 0 529 759
Size: 529 230
PasI CC'CwG_GG
Cuts at: 0 744 759
Size: 744 15
PfoI T'CCnGG_A
Cuts at: 0 209 759
Size: 209 550
PspOMI G'GGCC_C
Cuts at: 0 485 759
Size: 485 274
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 287 759
Size: 287 472
SfoI GGC'GCC
Cuts at: 0 256 759
Size: 256 503
SmaI CCC'GGG
Cuts at: 0 441 484 759
Size: 441 43 275
Fragments arranged by size:
441 275 43
SmlI C'TyrA_G
Cuts at: 0 466 759
Size: 466 293
SrfI GCCC'GGGC
Cuts at: 0 484 759
Size: 484 275
StuI AGG'CCT
Cuts at: 0 290 431 759
Size: 290 141 328
Fragments arranged by size:
328 290 141
StyI C'CwwG_G
Cuts at: 0 125 725 759
Size: 125 600 34
Fragments arranged by size:
600 125 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 688 759
Size: 118 570 71
Fragments arranged by size:
570 118 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 759
Size: 214 32 513
Fragments arranged by size:
513 214 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 693 732 759
Size: 693 39 27
Fragments arranged by size:
693 39 27
XmaI C'CCGG_G
Cuts at: 0 439 482 759
Size: 439 43 277
Fragments arranged by size:
439 277 43
XmnI GAAnn'nnTTC
Cuts at: 0 61 759
Size: 61 698
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BglI BglII Bme1580I BmgBI
BpmI BpuEI BsaHI BsaXI BseRI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstYI BtgI EaeI
EagI Eco57MI EcoNI EcoO109I FspI FspAI HaeII KasI
MscI MslI NaeI NarI NcoI NgoMIV PasI PfoI
PspOMI SfiI SfoI SmaI SmlI SrfI StuI StyI
TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BseYI
BsgI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI
EcoRI EcoRV FalI FseI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MspA1I NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TatI Tth111I XbaI XhoI ZraI