Restriction map for
pFN21AE6653
(Linear) (Six-base) MAPSORT of: KIEE6653.seq Check: 6030 from: 1 to: 744
KIEE6653.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 100 744
Size: 100 644
AlwNI CAG_nnn'CTG
Cuts at: 0 645 744
Size: 645 99
ApaI G_GGCC'C
Cuts at: 0 126 200 744
Size: 126 74 544
Fragments arranged by size:
544 126 74
ApoI r'AATT_y
Cuts at: 0 626 744
Size: 626 118
BamHI G'GATC_C
Cuts at: 0 388 744
Size: 388 356
BanI G'GyrC_C
Cuts at: 0 280 319 744
Size: 280 39 425
Fragments arranged by size:
425 280 39
BanII G_rGCy'C
Cuts at: 0 126 200 505 556 575 744
Size: 126 74 305 51 19 169
Fragments arranged by size:
305 169 126 74 51 19
BglI GCCn_nnn'nGGC
Cuts at: 0 317 673 744
Size: 317 356 71
Fragments arranged by size:
356 317 71
BglII A'GATC_T
Cuts at: 0 43 744
Size: 43 701
BlpI GC'TnA_GC
Cuts at: 0 261 744
Size: 261 483
Bme1580I G_kGCm'C
Cuts at: 0 126 200 744
Size: 126 74 544
Fragments arranged by size:
544 126 74
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 88 229 452 622 744
Size: 34 54 141 223 170 122
Fragments arranged by size:
223 170 141 122 54 34
Bpu10I CC'TnA_GC
Cuts at: 0 406 744
Size: 406 338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 744
Size: 706 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 331 744
Size: 103 228 413
Fragments arranged by size:
413 228 103
BsaWI w'CCGG_w
Cuts at: 0 494 548 744
Size: 494 54 196
Fragments arranged by size:
494 196 54
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 383 552 744
Size: 383 169 192
Fragments arranged by size:
383 192 169
BseYI C'CCAG_C
Cuts at: 0 586 744
Size: 586 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 744
Size: 159 585
BsiHKAI G_wGCw'C
Cuts at: 0 375 421 744
Size: 375 46 323
Fragments arranged by size:
375 323 46
Bsp1286I G_dGCh'C
Cuts at: 0 126 200 375 421 505 556 575 744
Size: 126 74 175 46 84 51 19 169
Fragments arranged by size:
175 169 126 84 74 51 46 19
BspEI T'CCGG_A
Cuts at: 0 494 548 744
Size: 494 54 196
Fragments arranged by size:
494 196 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 330 744
Size: 330 414
BstYI r'GATC_y
Cuts at: 0 43 388 744
Size: 43 345 356
Fragments arranged by size:
356 345 43
BtsI GCAGTG_nn'
Cuts at: 0 390 744
Size: 390 354
DraIII CAC_nnn'GTG
Cuts at: 0 326 744
Size: 326 418
EarI CTCTTCn'nnn_
Cuts at: 0 358 409 674 744
Size: 358 51 265 70
Fragments arranged by size:
358 265 70 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 375 691 744
Size: 375 316 53
Fragments arranged by size:
375 316 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 88 229 452 622 744
Size: 34 54 141 223 170 122
Fragments arranged by size:
223 170 141 122 54 34
EcoO109I rG'GnC_Cy
Cuts at: 0 26 122 123 131 196 197 240 744
Size: 26 96 1 8 65 1 43 504
Fragments arranged by size:
504 96 65 43 26 8 1 1
EcoRI G'AATT_C
Cuts at: 0 626 744
Size: 626 118
HincII GTy'rAC
Cuts at: 0 177 744
Size: 177 567
MspA1I CmG'CkG
Cuts at: 0 66 744
Size: 66 678
NspI r_CATG'y
Cuts at: 0 511 744
Size: 511 233
PasI CC'CwG_GG
Cuts at: 0 126 464 744
Size: 126 338 280
Fragments arranged by size:
338 280 126
PfoI T'CCnGG_A
Cuts at: 0 202 299 744
Size: 202 97 445
Fragments arranged by size:
445 202 97
PpuMI rG'GwC_Cy
Cuts at: 0 26 131 744
Size: 26 105 613
Fragments arranged by size:
613 105 26
PspOMI G'GGCC_C
Cuts at: 0 122 196 744
Size: 122 74 548
Fragments arranged by size:
548 122 74
PstI C_TGCA'G
Cuts at: 0 89 255 744
Size: 89 166 489
Fragments arranged by size:
489 166 89
PvuII CAG'CTG
Cuts at: 0 66 744
Size: 66 678
SanDI GG'GwC_CC
Cuts at: 0 131 744
Size: 131 613
SapI GCTCTTCn'nnn_
Cuts at: 0 409 744
Size: 409 335
SfcI C'TryA_G
Cuts at: 0 85 116 251 744
Size: 85 31 135 493
Fragments arranged by size:
493 135 85 31
SmlI C'TyrA_G
Cuts at: 0 721 744
Size: 721 23
StyI C'CwwG_G
Cuts at: 0 708 744
Size: 708 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 268 530 744
Size: 268 262 214
Fragments arranged by size:
268 262 214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 744
Size: 330 414
Enzymes that do cut:
AlfI AlwNI ApaI ApoI BamHI BanI BanII BglI
BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaWI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BstXI BstYI
BtsI DraIII EarI EciI Eco57MI EcoO109I EcoRI HincII
MspA1I NspI PasI PfoI PpuMI PspOMI PstI PvuII
SanDI SapI SfcI SmlI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BmgBI BmrI BmtI BplI BsaAI
BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DrdI EaeI EagI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI