Restriction map for
pFN21AE6652
(Linear) (Six-base) MAPSORT of: KIEE6652.seq Check: 6654 from: 1 to: 735
KIEE6652.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AatII G_ACGT'C
Cuts at: 0 668 735
Size: 668 67
AccI GT'mk_AC
Cuts at: 0 413 735
Size: 413 322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 500 735
Size: 457 43 235
Fragments arranged by size:
457 235 43
AlwNI CAG_nnn'CTG
Cuts at: 0 594 735
Size: 594 141
BclI T'GATC_A
Cuts at: 0 467 735
Size: 467 268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 293 735
Size: 70 223 442
Fragments arranged by size:
442 223 70
Bpu10I CC'TnA_GC
Cuts at: 0 637 735
Size: 637 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 735
Size: 584 151
BsaAI yAC'GTr
Cuts at: 0 543 624 735
Size: 543 81 111
Fragments arranged by size:
543 111 81
BsaHI Gr'CG_yC
Cuts at: 0 320 665 735
Size: 320 345 70
Fragments arranged by size:
345 320 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 735
Size: 196 30 509
Fragments arranged by size:
509 196 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 715 735
Size: 715 20
BseYI C'CCAG_C
Cuts at: 0 125 735
Size: 125 610
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 646 735
Size: 53 593 89
Fragments arranged by size:
593 89 53
BsiHKAI G_wGCw'C
Cuts at: 0 514 735
Size: 514 221
BsmI GAATG_Cn'
Cuts at: 0 195 735
Size: 195 540
Bsp1286I G_dGCh'C
Cuts at: 0 514 735
Size: 514 221
BsrDI GCAATG_nn'
Cuts at: 0 631 735
Size: 631 104
BstAPI GCAn_nnn'nTGC
Cuts at: 0 633 735
Size: 633 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 271 735
Size: 271 464
BstYI r'GATC_y
Cuts at: 0 432 735
Size: 432 303
BtgI C'CryG_G
Cuts at: 0 547 609 735
Size: 547 62 126
Fragments arranged by size:
547 126 62
DraI TTT'AAA
Cuts at: 0 107 735
Size: 107 628
EarI CTCTTCn'nnn_
Cuts at: 0 519 735
Size: 519 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 293 457 500 735
Size: 70 223 164 43 235
Fragments arranged by size:
235 223 164 70 43
EcoO109I rG'GnC_Cy
Cuts at: 0 591 735
Size: 591 144
MslI CAynn'nnrTG
Cuts at: 0 205 735
Size: 205 530
MspA1I CmG'CkG
Cuts at: 0 389 658 735
Size: 389 269 77
Fragments arranged by size:
389 269 77
NcoI C'CATG_G
Cuts at: 0 547 735
Size: 547 188
NspI r_CATG'y
Cuts at: 0 247 735
Size: 247 488
PmlI CAC'GTG
Cuts at: 0 543 624 735
Size: 543 81 111
Fragments arranged by size:
543 111 81
PstI C_TGCA'G
Cuts at: 0 350 735
Size: 350 385
SapI GCTCTTCn'nnn_
Cuts at: 0 519 735
Size: 519 216
SfcI C'TryA_G
Cuts at: 0 346 735
Size: 346 389
SmlI C'TyrA_G
Cuts at: 0 599 735
Size: 599 136
StyI C'CwwG_G
Cuts at: 0 182 547 735
Size: 182 365 188
Fragments arranged by size:
365 188 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 233 512 735
Size: 233 279 223
Fragments arranged by size:
279 233 223
TatI w'GTAC_w
Cuts at: 0 290 735
Size: 290 445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 735
Size: 196 32 507
Fragments arranged by size:
507 196 32
ZraI GAC'GTC
Cuts at: 0 666 735
Size: 666 69
Enzymes that do cut:
AatII AccI AcuI AlwNI BclI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BsrDI BstAPI BstXI BstYI BtgI DraI EarI
Eco57MI EcoO109I MslI MspA1I NcoI NspI PmlI PstI
SapI SfcI SmlI StyI TaqII TatI TstI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BsaI BsaBI BsaWI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI