Restriction map for
pFN21AE6651
(Linear) (Six-base) MAPSORT of: KIEE6651.seq Check: 3796 from: 1 to: 738
KIEE6651.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AatII G_ACGT'C
Cuts at: 0 159 738
Size: 159 579
AccI GT'mk_AC
Cuts at: 0 177 738
Size: 177 561
AclI AA'CG_TT
Cuts at: 0 296 738
Size: 296 442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 738
Size: 172 566
AhdI GACnn_n'nnGTC
Cuts at: 0 669 738
Size: 669 69
ApoI r'AATT_y
Cuts at: 0 414 686 738
Size: 414 272 52
Fragments arranged by size:
414 272 52
AvaI C'yCGr_G
Cuts at: 0 67 738
Size: 67 671
BamHI G'GATC_C
Cuts at: 0 635 738
Size: 635 103
BanI G'GyrC_C
Cuts at: 0 56 738
Size: 56 682
BclI T'GATC_A
Cuts at: 0 705 738
Size: 705 33
BglII A'GATC_T
Cuts at: 0 595 738
Size: 595 143
Bme1580I G_kGCm'C
Cuts at: 0 47 738
Size: 47 691
BmrI ACTGGGnnnn_n'
Cuts at: 0 412 738
Size: 412 326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 738
Size: 430 308
BsaI GGTCTCn'nnnn_
Cuts at: 0 610 738
Size: 610 128
BsaHI Gr'CG_yC
Cuts at: 0 156 738
Size: 156 582
BsaWI w'CCGG_w
Cuts at: 0 698 738
Size: 698 40
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 129 738
Size: 118 11 609
Fragments arranged by size:
609 118 11
BseYI C'CCAG_C
Cuts at: 0 5 738
Size: 5 733
BsmBI CGTCTCn'nnnn_
Cuts at: 0 163 738
Size: 163 575
Bsp1286I G_dGCh'C
Cuts at: 0 47 738
Size: 47 691
BsrFI r'CCGG_y
Cuts at: 0 59 738
Size: 59 679
BstXI CCAn_nnnn'nTGG
Cuts at: 0 226 738
Size: 226 512
BstYI r'GATC_y
Cuts at: 0 135 595 635 738
Size: 135 460 40 103
Fragments arranged by size:
460 135 103 40
BtsI GCAGTG_nn'
Cuts at: 0 20 738
Size: 20 718
EarI CTCTTCn'nnn_
Cuts at: 0 332 539 738
Size: 332 207 199
Fragments arranged by size:
332 207 199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 738
Size: 172 566
EcoRI G'AATT_C
Cuts at: 0 414 686 738
Size: 414 272 52
Fragments arranged by size:
414 272 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 120 152 435 467 655 687 738
Size: 120 32 283 32 188 32 51
Fragments arranged by size:
283 188 120 51 32 32 32
HincII GTy'rAC
Cuts at: 0 178 294 738
Size: 178 116 444
Fragments arranged by size:
444 178 116
HpaI GTT'AAC
Cuts at: 0 294 738
Size: 294 444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 738
Size: 546 192
MslI CAynn'nnrTG
Cuts at: 0 434 738
Size: 434 304
MspA1I CmG'CkG
Cuts at: 0 267 676 738
Size: 267 409 62
Fragments arranged by size:
409 267 62
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 281 313 738
Size: 281 32 425
Fragments arranged by size:
425 281 32
PvuII CAG'CTG
Cuts at: 0 267 676 738
Size: 267 409 62
Fragments arranged by size:
409 267 62
SalI G'TCGA_C
Cuts at: 0 176 738
Size: 176 562
SmaI CCC'GGG
Cuts at: 0 69 738
Size: 69 669
SmlI C'TyrA_G
Cuts at: 0 409 738
Size: 409 329
SrfI GCCC'GGGC
Cuts at: 0 69 738
Size: 69 669
XmaI C'CCGG_G
Cuts at: 0 67 738
Size: 67 671
ZraI GAC'GTC
Cuts at: 0 157 738
Size: 157 581
Enzymes that do cut:
AatII AccI AclI AcuI AhdI ApoI AvaI BamHI
BanI BclI BglII Bme1580I BmrI BpuEI BsaI BsaHI
BsaWI BseRI BseYI BsmBI Bsp1286I BsrFI BstXI BstYI
BtsI EarI Eco57MI EcoRI Hin4I HincII HpaI MmeI
MslI MspA1I PpiI PvuII SalI SmaI SmlI SrfI
XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BlpI BmgBI BmtI BplI BpmI Bpu10I BsaAI
BsaBI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI