Restriction map for
pFN21AE6650
(Linear) (Six-base) MAPSORT of: KIEE6650.seq Check: 6775 from: 1 to: 702
KIEE6650.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 307 414 702
Size: 307 107 288
Fragments arranged by size:
307 288 107
AatII G_ACGT'C
Cuts at: 0 686 702
Size: 686 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 516 702
Size: 61 455 186
Fragments arranged by size:
455 186 61
AfeI AGC'GCT
Cuts at: 0 38 702
Size: 38 664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 664 702
Size: 136 528 38
Fragments arranged by size:
528 136 38
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 702
Size: 538 32 132
Fragments arranged by size:
538 132 32
AlwNI CAG_nnn'CTG
Cuts at: 0 204 393 678 702
Size: 204 189 285 24
Fragments arranged by size:
285 204 189 24
ApaI G_GGCC'C
Cuts at: 0 622 702
Size: 622 80
AvaI C'yCGr_G
Cuts at: 0 463 622 702
Size: 463 159 80
Fragments arranged by size:
463 159 80
BanII G_rGCy'C
Cuts at: 0 554 622 702
Size: 554 68 80
Fragments arranged by size:
554 80 68
BbvCI CC'TCA_GC
Cuts at: 0 355 702
Size: 355 347
BclI T'GATC_A
Cuts at: 0 599 702
Size: 599 103
BglI GCCn_nnn'nGGC
Cuts at: 0 173 702
Size: 173 529
BlpI GC'TnA_GC
Cuts at: 0 592 702
Size: 592 110
Bme1580I G_kGCm'C
Cuts at: 0 575 622 702
Size: 575 47 80
Fragments arranged by size:
575 80 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 190 226 253 702
Size: 109 81 36 27 449
Fragments arranged by size:
449 109 81 36 27
Bpu10I CC'TnA_GC
Cuts at: 0 104 355 702
Size: 104 251 347
Fragments arranged by size:
347 251 104
BsaAI yAC'GTr
Cuts at: 0 271 372 474 579 702
Size: 271 101 102 105 123
Fragments arranged by size:
271 123 105 102 101
BsaHI Gr'CG_yC
Cuts at: 0 281 488 683 702
Size: 281 207 195 19
Fragments arranged by size:
281 207 195 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 702
Size: 538 30 134
Fragments arranged by size:
538 134 30
BseYI C'CCAG_C
Cuts at: 0 51 644 702
Size: 51 593 58
Fragments arranged by size:
593 58 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 534 702
Size: 217 317 168
Fragments arranged by size:
317 217 168
BsiHKAI G_wGCw'C
Cuts at: 0 554 702
Size: 554 148
Bsp1286I G_dGCh'C
Cuts at: 0 554 575 622 702
Size: 554 21 47 80
Fragments arranged by size:
554 80 47 21
BspHI T'CATG_A
Cuts at: 0 323 542 702
Size: 323 219 160
Fragments arranged by size:
323 219 160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 233 307 414 638 702
Size: 233 74 107 224 64
Fragments arranged by size:
233 224 107 74 64
BsrBI CCG'CTC
Cuts at: 0 215 702
Size: 215 487
BssHII G'CGCG_C
Cuts at: 0 315 633 635 637 702
Size: 315 318 2 2 65
Fragments arranged by size:
318 315 65 2 2
BstEII G'GTnAC_C
Cuts at: 0 582 702
Size: 582 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 702
Size: 411 291
BtgI C'CryG_G
Cuts at: 0 74 258 702
Size: 74 184 444
Fragments arranged by size:
444 184 74
EcoICRI GAG'CTC
Cuts at: 0 552 702
Size: 552 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 109 190 226 253 516 702
Size: 61 48 81 36 27 263 186
Fragments arranged by size:
263 186 81 61 48 36 27
EcoO109I rG'GnC_Cy
Cuts at: 0 390 619 627 702
Size: 390 229 8 75
Fragments arranged by size:
390 229 75 8
FspI TGC'GCA
Cuts at: 0 160 702
Size: 160 542
HaeII r_GCGC'y
Cuts at: 0 40 168 241 702
Size: 40 128 73 461
Fragments arranged by size:
461 128 73 40
HincII GTy'rAC
Cuts at: 0 144 702
Size: 144 558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 428 702
Size: 302 126 274
Fragments arranged by size:
302 274 126
MslI CAynn'nnrTG
Cuts at: 0 541 702
Size: 541 161
MspA1I CmG'CkG
Cuts at: 0 260 308 648 675 702
Size: 260 48 340 27 27
Fragments arranged by size:
340 260 48 27 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 702
Size: 393 309
PfoI T'CCnGG_A
Cuts at: 0 203 702
Size: 203 499
PmlI CAC'GTG
Cuts at: 0 474 702
Size: 474 228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 702
Size: 538 32 132
Fragments arranged by size:
538 132 32
PpuMI rG'GwC_Cy
Cuts at: 0 627 702
Size: 627 75
PspOMI G'GGCC_C
Cuts at: 0 618 702
Size: 618 84
PstI C_TGCA'G
Cuts at: 0 293 674 702
Size: 293 381 28
Fragments arranged by size:
381 293 28
PvuII CAG'CTG
Cuts at: 0 308 648 675 702
Size: 308 340 27 27
Fragments arranged by size:
340 308 27 27
SacI G_AGCT'C
Cuts at: 0 554 702
Size: 554 148
SacII CC_GC'GG
Cuts at: 0 261 702
Size: 261 441
SfcI C'TryA_G
Cuts at: 0 289 670 702
Size: 289 381 32
Fragments arranged by size:
381 289 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 658 702
Size: 658 44
TatI w'GTAC_w
Cuts at: 0 347 702
Size: 347 355
ZraI GAC'GTC
Cuts at: 0 684 702
Size: 684 18
Enzymes that do cut:
AarI AatII AcuI AfeI AlfI AloI AlwNI ApaI
AvaI BanII BbvCI BclI BglI BlpI Bme1580I BpmI
Bpu10I BsaAI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BssHII BstEII BstXI BtgI EcoICRI
Eco57MI EcoO109I FspI HaeII HincII MmeI MslI MspA1I
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SacII SfcI TaqII TatI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AleI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BcgI BciVI BfrBI BglII BmgBI
BmrI BmtI BplI BpuEI BsaI BsaBI BsaWI BseRI
BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI