Restriction map for
pFN21AE6648
(Linear) (Six-base) MAPSORT of: KIEE6648.seq Check: 2011 from: 1 to: 669
KIEE6648.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 407 669
Size: 407 262
Acc65I G'GTAC_C
Cuts at: 0 283 669
Size: 283 386
AhdI GACnn_n'nnGTC
Cuts at: 0 164 669
Size: 164 505
AlwNI CAG_nnn'CTG
Cuts at: 0 305 669
Size: 305 364
ApaI G_GGCC'C
Cuts at: 0 435 669
Size: 435 234
AscI GG'CGCG_CC
Cuts at: 0 521 669
Size: 521 148
AvaI C'yCGr_G
Cuts at: 0 230 669
Size: 230 439
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 267 275 300 308 669
Size: 267 8 25 8 361
Fragments arranged by size:
361 267 25 8 8
BanI G'GyrC_C
Cuts at: 0 11 283 370 632 669
Size: 11 272 87 262 37
Fragments arranged by size:
272 262 87 37 11
BanII G_rGCy'C
Cuts at: 0 435 669
Size: 435 234
BbeI G_GCGC'C
Cuts at: 0 374 636 669
Size: 374 262 33
Fragments arranged by size:
374 262 33
Bme1580I G_kGCm'C
Cuts at: 0 316 435 669
Size: 316 119 234
Fragments arranged by size:
316 234 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 194 669
Size: 194 475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 147 397 436 604 669
Size: 27 120 250 39 168 65
Fragments arranged by size:
250 168 120 65 39 27
BsaHI Gr'CG_yC
Cuts at: 0 371 457 633 669
Size: 371 86 176 36
Fragments arranged by size:
371 176 86 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 145 175 669
Size: 145 30 494
Fragments arranged by size:
494 145 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 669
Size: 226 443
BseYI C'CCAG_C
Cuts at: 0 200 294 526 669
Size: 200 94 232 143
Fragments arranged by size:
232 200 143 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 544 669
Size: 421 123 125
Fragments arranged by size:
421 125 123
BsiEI CG_ry'CG
Cuts at: 0 262 333 669
Size: 262 71 336
Fragments arranged by size:
336 262 71
BsiHKAI G_wGCw'C
Cuts at: 0 383 669
Size: 383 286
BsmI GAATG_Cn'
Cuts at: 0 370 669
Size: 370 299
Bsp1286I G_dGCh'C
Cuts at: 0 316 383 435 669
Size: 316 67 52 234
Fragments arranged by size:
316 234 67 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 295 407 669
Size: 295 112 262
Fragments arranged by size:
295 262 112
BsrBI CCG'CTC
Cuts at: 0 150 219 630 669
Size: 150 69 411 39
Fragments arranged by size:
411 150 69 39
BsrFI r'CCGG_y
Cuts at: 0 328 669
Size: 328 341
BssHII G'CGCG_C
Cuts at: 0 521 669
Size: 521 148
BstAPI GCAn_nnn'nTGC
Cuts at: 0 305 365 669
Size: 305 60 304
Fragments arranged by size:
305 304 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 646 669
Size: 646 23
BtsI GCAGTG_nn'
Cuts at: 0 366 438 669
Size: 366 72 231
Fragments arranged by size:
366 231 72
EaeI y'GGCC_r
Cuts at: 0 259 330 669
Size: 259 71 339
Fragments arranged by size:
339 259 71
EagI C'GGCC_G
Cuts at: 0 259 330 669
Size: 259 71 339
Fragments arranged by size:
339 259 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 147 397 436 604 669
Size: 27 120 250 39 168 65
Fragments arranged by size:
250 168 120 65 39 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 642 669
Size: 642 27
EcoO109I rG'GnC_Cy
Cuts at: 0 431 432 669
Size: 431 1 237
Fragments arranged by size:
431 237 1
FspI TGC'GCA
Cuts at: 0 552 669
Size: 552 117
HaeII r_GCGC'y
Cuts at: 0 194 374 515 636 669
Size: 194 180 141 121 33
Fragments arranged by size:
194 180 141 121 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 127 150 182 235 267 669
Size: 95 32 23 32 53 32 402
Fragments arranged by size:
402 95 53 32 32 32 23
KasI G'GCGC_C
Cuts at: 0 370 632 669
Size: 370 262 37
Fragments arranged by size:
370 262 37
KpnI G_GTAC'C
Cuts at: 0 287 669
Size: 287 382
MspA1I CmG'CkG
Cuts at: 0 467 624 669
Size: 467 157 45
Fragments arranged by size:
467 157 45
NarI GG'CG_CC
Cuts at: 0 371 633 669
Size: 371 262 36
Fragments arranged by size:
371 262 36
NotI GC'GGCC_GC
Cuts at: 0 259 669
Size: 259 410
PspOMI G'GGCC_C
Cuts at: 0 431 669
Size: 431 238
PstI C_TGCA'G
Cuts at: 0 274 418 669
Size: 274 144 251
Fragments arranged by size:
274 251 144
SfcI C'TryA_G
Cuts at: 0 270 414 669
Size: 270 144 255
Fragments arranged by size:
270 255 144
SfoI GGC'GCC
Cuts at: 0 372 634 669
Size: 372 262 35
Fragments arranged by size:
372 262 35
SmaI CCC'GGG
Cuts at: 0 232 669
Size: 232 437
StyI C'CwwG_G
Cuts at: 0 116 669
Size: 116 553
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 587 669
Size: 587 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 583 615 669
Size: 583 32 54
Fragments arranged by size:
583 54 32
XmaI C'CCGG_G
Cuts at: 0 230 669
Size: 230 439
XmnI GAAnn'nnTTC
Cuts at: 0 481 669
Size: 481 188
Enzymes that do cut:
AarI Acc65I AhdI AlwNI ApaI AscI AvaI BaeI
BanI BanII BbeI Bme1580I BmrI BpmI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BssHII BstAPI BtgZI BtsI EaeI EagI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI KpnI
MspA1I NarI NotI PspOMI PstI SfcI SfoI SmaI
StyI TaqII TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrDI
BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI MfeI MluI MmeI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XcmI XhoI ZraI