(Linear) (Six-base) MAPSORT of: KIEE6647.seq  Check: 8237  from: 1  to: 696



KIEE6647.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AccI GT'mk_AC

Cuts at:      0     117     696 
   Size:        117     579
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     542     696 
   Size:        170     372     154

  Fragments arranged by size: 

                 372     170     154
 
AflIII A'CryG_T

Cuts at:      0     382     571     655     696 
   Size:        382     189      84      41

  Fragments arranged by size: 

                 382     189      84      41
 
AlwNI CAG_nnn'CTG

Cuts at:      0     136     696 
   Size:        136     560
 
ApoI r'AATT_y

Cuts at:      0     552     687     696 
   Size:        552     135       9

  Fragments arranged by size: 

                 552     135       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     349     696 
   Size:        349     347
 
BfrBI ATG'CAT

Cuts at:      0     419     696 
   Size:        419     277
 
BglI GCCn_nnn'nGGC

Cuts at:      0     347     696 
   Size:        347     349
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     207     696 
   Size:        207     489
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     407     696 
   Size:        407     289
 
BsiHKAI G_wGCw'C

Cuts at:      0     636     696 
   Size:        636      60
 
BsmI GAATG_Cn'

Cuts at:      0     625     696 
   Size:        625      71
 
Bsp1286I G_dGCh'C

Cuts at:      0     636     696 
   Size:        636      60
 
BspHI T'CATG_A

Cuts at:      0     102     696 
   Size:        102     594
 
BsrDI GCAATG_nn'

Cuts at:      0     210     696 
   Size:        210     486
 
BsrGI T'GTAC_A

Cuts at:      0     312     652     696 
   Size:        312     340      44

  Fragments arranged by size: 

                 340     312      44
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     631     696 
   Size:        631      65
 
BstEII G'GTnAC_C

Cuts at:      0     230     696 
   Size:        230     466
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     208     243     696 
   Size:        208      35     453

  Fragments arranged by size: 

                 453     208      35
 
DraI TTT'AAA

Cuts at:      0     500     696 
   Size:        500     196
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     542     696 
   Size:        170     372     154

  Fragments arranged by size: 

                 372     170     154
 
NsiI A_TGCA'T

Cuts at:      0     421     696 
   Size:        421     275
 
NspI r_CATG'y

Cuts at:      0     386     437     575     659     696 
   Size:        386      51     138      84      37

  Fragments arranged by size: 

                 386     138      84      51      37
 
PciI A'CATG_T

Cuts at:      0     382     571     655     696 
   Size:        382     189      84      41

  Fragments arranged by size: 

                 382     189      84      41
 
SfcI C'TryA_G

Cuts at:      0     137     326     696 
   Size:        137     189     370

  Fragments arranged by size: 

                 370     189     137
 
SspI AAT'ATT

Cuts at:      0     148     696 
   Size:        148     548
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     215     696 
   Size:        215     481
 
TatI w'GTAC_w

Cuts at:      0     312     652     696 
   Size:        312     340      44

  Fragments arranged by size: 

                 340     312      44
 
XmnI GAAnn'nnTTC

Cuts at:      0     555     696 
   Size:        555     141
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI     BbsI    BfrBI     BglI
     BmrI     BsaI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BsrGI
   BstAPI   BstEII    CspCI     DraI  Eco57MI     NsiI     NspI     PciI
     SfcI     SspI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI