(Linear) (Six-base) MAPSORT of: KIEE6646.seq  Check: 731  from: 1  to: 678



KIEE6646.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     349     678 
   Size:        349     329
 
Acc65I G'GTAC_C

Cuts at:      0     614     678 
   Size:        614      64
 
AfeI AGC'GCT

Cuts at:      0     591     678 
   Size:        591      87
 
ApoI r'AATT_y

Cuts at:      0     438     678 
   Size:        438     240
 
BanI G'GyrC_C

Cuts at:      0     313     614     678 
   Size:        313     301      64

  Fragments arranged by size: 

                 313     301      64
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     113     564     678 
   Size:        113     451     114

  Fragments arranged by size: 

                 451     114     113
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     563     678 
   Size:        563     115
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     678 
   Size:        356     322
 
BseYI C'CCAG_C

Cuts at:      0     208     678 
   Size:        208     470
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     678 
   Size:        377     301
 
BsiHKAI G_wGCw'C

Cuts at:      0     488     678 
   Size:        488     190
 
BsmI GAATG_Cn'

Cuts at:      0     226     669     678 
   Size:        226     443       9

  Fragments arranged by size: 

                 443     226       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     488     678 
   Size:        488     190
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     349     678 
   Size:        349     329
 
BstYI r'GATC_y

Cuts at:      0     451     678 
   Size:        451     227
 
BtsI GCAGTG_nn'

Cuts at:      0     231     339     678 
   Size:        231     108     339

  Fragments arranged by size: 

                 339     231     108
 
EaeI y'GGCC_r

Cuts at:      0      98     414     678 
   Size:         98     316     264

  Fragments arranged by size: 

                 316     264      98
 
EarI CTCTTCn'nnn_

Cuts at:      0     412     678 
   Size:        412     266
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     678 
   Size:        356     322
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     423     678 
   Size:        423     255
 
HaeII r_GCGC'y

Cuts at:      0     593     678 
   Size:        593      85
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     443     475     678 
   Size:        443      32     203

  Fragments arranged by size: 

                 443     203      32
 
HincII GTy'rAC

Cuts at:      0     279     626     678 
   Size:        279     347      52

  Fragments arranged by size: 

                 347     279      52
 
KpnI G_GTAC'C

Cuts at:      0     618     678 
   Size:        618      60
 
MscI TGG'CCA

Cuts at:      0     100     416     678 
   Size:        100     316     262

  Fragments arranged by size: 

                 316     262     100
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     128     678 
   Size:        128     550
 
SapI GCTCTTCn'nnn_

Cuts at:      0     412     678 
   Size:        412     266
 
SspI AAT'ATT

Cuts at:      0     175     678 
   Size:        175     503
 
TatI w'GTAC_w

Cuts at:      0     562     678 
   Size:        562     116
 
Tth111I GACn'n_nGTC

Cuts at:      0     630     678 
   Size:        630      48
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     380     678 
   Size:        380     298
 
XmnI GAAnn'nnTTC

Cuts at:      0     406     678 
   Size:        406     272
 

 Enzymes that do cut:

     AarI   Acc65I     AfeI     ApoI     BanI     BmrI     BplI     BpmI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BstYI     BtsI
     EaeI     EarI  Eco57MI     FalI    HaeII    Hin4I   HincII     KpnI
     MscI    PflMI     SapI     SspI     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TstI
     XbaI     XhoI     XmaI     ZraI