Restriction map for
pFN21AE6644
(Linear) (Six-base) MAPSORT of: KIEE6644.seq Check: 222 from: 1 to: 726
KIEE6644.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 591 726
Size: 591 135
AflIII A'CryG_T
Cuts at: 0 275 726
Size: 275 451
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 726
Size: 316 32 378
Fragments arranged by size:
378 316 32
ApaI G_GGCC'C
Cuts at: 0 99 182 660 726
Size: 99 83 478 66
Fragments arranged by size:
478 99 83 66
ApaLI G'TGCA_C
Cuts at: 0 634 726
Size: 634 92
AvaI C'yCGr_G
Cuts at: 0 99 164 197 256 305 356 726
Size: 99 65 33 59 49 51 370
Fragments arranged by size:
370 99 65 59 51 49 33
BamHI G'GATC_C
Cuts at: 0 416 726
Size: 416 310
BanI G'GyrC_C
Cuts at: 0 104 726
Size: 104 622
BanII G_rGCy'C
Cuts at: 0 99 182 263 660 726
Size: 99 83 81 397 66
Fragments arranged by size:
397 99 83 81 66
BbeI G_GCGC'C
Cuts at: 0 108 726
Size: 108 618
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 247 281 726
Size: 247 34 445
Fragments arranged by size:
445 247 34
Bme1580I G_kGCm'C
Cuts at: 0 99 182 638 660 726
Size: 99 83 456 22 66
Fragments arranged by size:
456 99 83 66 22
BmgBI CAC'GTC
Cuts at: 0 315 726
Size: 315 411
BmrI ACTGGGnnnn_n'
Cuts at: 0 465 726
Size: 465 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 318 726
Size: 9 309 408
Fragments arranged by size:
408 309 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 358 726
Size: 358 368
BsaAI yAC'GTr
Cuts at: 0 276 633 726
Size: 276 357 93
Fragments arranged by size:
357 276 93
BsaHI Gr'CG_yC
Cuts at: 0 49 105 726
Size: 49 56 621
Fragments arranged by size:
621 56 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 208 238 316 346 726
Size: 208 30 78 30 380
Fragments arranged by size:
380 208 78 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 150 376 422 726
Size: 10 140 226 46 304
Fragments arranged by size:
304 226 140 46 10
BsiHKAI G_wGCw'C
Cuts at: 0 263 374 489 638 726
Size: 263 111 115 149 88
Fragments arranged by size:
263 149 115 111 88
BsmBI CGTCTCn'nnnn_
Cuts at: 0 56 726
Size: 56 670
Bsp1286I G_dGCh'C
Cuts at: 0 99 182 263 374 489 638 660 726
Size: 99 83 81 111 115 149 22 66
Fragments arranged by size:
149 115 111 99 83 81 66 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 591 726
Size: 591 135
BsrFI r'CCGG_y
Cuts at: 0 187 726
Size: 187 539
BstEII G'GTnAC_C
Cuts at: 0 553 625 726
Size: 553 72 101
Fragments arranged by size:
553 101 72
BstYI r'GATC_y
Cuts at: 0 416 726
Size: 416 310
BtgI C'CryG_G
Cuts at: 0 287 476 726
Size: 287 189 250
Fragments arranged by size:
287 250 189
EaeI y'GGCC_r
Cuts at: 0 146 726
Size: 146 580
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 409 691 726
Size: 409 282 35
Fragments arranged by size:
409 282 35
EcoICRI GAG'CTC
Cuts at: 0 261 726
Size: 261 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 318 726
Size: 9 309 408
Fragments arranged by size:
408 309 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 12 726
Size: 12 714
EcoO109I rG'GnC_Cy
Cuts at: 0 96 168 656 657 726
Size: 96 72 488 1 69
Fragments arranged by size:
488 96 72 69 1
HaeII r_GCGC'y
Cuts at: 0 108 726
Size: 108 618
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 401 433 726
Size: 401 32 293
Fragments arranged by size:
401 293 32
KasI G'GCGC_C
Cuts at: 0 104 726
Size: 104 622
MspA1I CmG'CkG
Cuts at: 0 87 726
Size: 87 639
NaeI GCC'GGC
Cuts at: 0 189 726
Size: 189 537
NarI GG'CG_CC
Cuts at: 0 105 726
Size: 105 621
NcoI C'CATG_G
Cuts at: 0 287 476 726
Size: 287 189 250
Fragments arranged by size:
287 250 189
NgoMIV G'CCGG_C
Cuts at: 0 187 726
Size: 187 539
NspI r_CATG'y
Cuts at: 0 475 726
Size: 475 251
PfoI T'CCnGG_A
Cuts at: 0 575 726
Size: 575 151
PmlI CAC'GTG
Cuts at: 0 633 726
Size: 633 93
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 726
Size: 316 32 378
Fragments arranged by size:
378 316 32
PpuMI rG'GwC_Cy
Cuts at: 0 168 726
Size: 168 558
PspOMI G'GGCC_C
Cuts at: 0 95 178 656 726
Size: 95 83 478 70
Fragments arranged by size:
478 95 83 70
PspXI vC'TCGA_Gb
Cuts at: 0 256 726
Size: 256 470
PstI C_TGCA'G
Cuts at: 0 398 428 726
Size: 398 30 298
Fragments arranged by size:
398 298 30
SacI G_AGCT'C
Cuts at: 0 263 726
Size: 263 463
SanDI GG'GwC_CC
Cuts at: 0 168 726
Size: 168 558
SbfI CC_TGCA'GG
Cuts at: 0 428 726
Size: 428 298
SfcI C'TryA_G
Cuts at: 0 394 424 726
Size: 394 30 302
Fragments arranged by size:
394 302 30
SfoI GGC'GCC
Cuts at: 0 106 726
Size: 106 620
SmaI CCC'GGG
Cuts at: 0 101 166 307 358 726
Size: 101 65 141 51 368
Fragments arranged by size:
368 141 101 65 51
SmlI C'TyrA_G
Cuts at: 0 256 726
Size: 256 470
SphI G_CATG'C
Cuts at: 0 475 726
Size: 475 251
StyI C'CwwG_G
Cuts at: 0 110 287 476 702 726
Size: 110 177 189 226 24
Fragments arranged by size:
226 189 177 110 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 382 726
Size: 382 344
TatI w'GTAC_w
Cuts at: 0 278 726
Size: 278 448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 208 240 726
Size: 208 32 486
Fragments arranged by size:
486 208 32
XhoI C'TCGA_G
Cuts at: 0 256 726
Size: 256 470
XmaI C'CCGG_G
Cuts at: 0 99 164 305 356 726
Size: 99 65 141 51 370
Fragments arranged by size:
370 141 99 65 51
XmnI GAAnn'nnTTC
Cuts at: 0 328 726
Size: 328 398
Enzymes that do cut:
AarI AflIII AloI ApaI ApaLI AvaI BamHI BanI
BanII BbeI BcgI Bme1580I BmgBI BmrI BpmI BsaI
BsaAI BsaHI BsaXI BseRI BsiHKAI BsmBI Bsp1286I BspMI
BsrFI BstEII BstYI BtgI EaeI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MspA1I NaeI NarI
NcoI NgoMIV NspI PfoI PmlI PpiI PpuMI PspOMI
PspXI PstI SacI SanDI SbfI SfcI SfoI SmaI
SmlI SphI StyI TaqII TatI TstI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaBI BsaWI
BseYI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PshAI PsiI PsrI PvuI PvuII
RsrII SacII SalI SapI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XcmI ZraI