(Linear) (Six-base) MAPSORT of: KIEE6643.seq  Check: 1941  from: 1  to: 978



KIEE6643.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

Acc65I G'GTAC_C

Cuts at:      0      70     143     978 
   Size:         70      73     835

  Fragments arranged by size: 

                 835      73      70
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     896     978 
   Size:        896      82
 
ApoI r'AATT_y

Cuts at:      0     619     846     978 
   Size:        619     227     132

  Fragments arranged by size: 

                 619     227     132
 
BanI G'GyrC_C

Cuts at:      0      70     143     978 
   Size:         70      73     835

  Fragments arranged by size: 

                 835      73      70
 
BanII G_rGCy'C

Cuts at:      0     533     978 
   Size:        533     445
 
BglII A'GATC_T

Cuts at:      0     854     978 
   Size:        854     124
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     325     547     978 
   Size:        310      15     222     431

  Fragments arranged by size: 

                 431     310     222      15
 
BseYI C'CCAG_C

Cuts at:      0     602     978 
   Size:        602     376
 
BsiHKAI G_wGCw'C

Cuts at:      0     533     978 
   Size:        533     445
 
BsmI GAATG_Cn'

Cuts at:      0      50     978 
   Size:         50     928
 
Bsp1286I G_dGCh'C

Cuts at:      0     533     978 
   Size:        533     445
 
BspHI T'CATG_A

Cuts at:      0     966     978 
   Size:        966      12
 
BstYI r'GATC_y

Cuts at:      0       3     342     854     978 
   Size:          3     339     512     124

  Fragments arranged by size: 

                 512     339     124       3
 
Bsu36I CC'TnA_GG

Cuts at:      0      98     978 
   Size:         98     880
 
BtgI C'CryG_G

Cuts at:      0     365     978 
   Size:        365     613
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     472     978 
   Size:        472     506
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     283     978 
   Size:        283     695
 
EcoICRI GAG'CTC

Cuts at:      0     531     978 
   Size:        531     447
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     325     547     896     978 
   Size:        310      15     222     349      82

  Fragments arranged by size: 

                 349     310     222      82      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     161     321     978 
   Size:        161     160     657

  Fragments arranged by size: 

                 657     161     160
 
EcoRV GAT'ATC

Cuts at:      0     243     978 
   Size:        243     735
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     750     782     978 
   Size:        750      32     196

  Fragments arranged by size: 

                 750     196      32
 
HindIII A'AGCT_T

Cuts at:      0     464     819     978 
   Size:        464     355     159

  Fragments arranged by size: 

                 464     355     159
 
KpnI G_GTAC'C

Cuts at:      0      74     147     978 
   Size:         74      73     831

  Fragments arranged by size: 

                 831      74      73
 
MslI CAynn'nnrTG

Cuts at:      0     155     978 
   Size:        155     823
 
MspA1I CmG'CkG

Cuts at:      0     525     978 
   Size:        525     453
 
NcoI C'CATG_G

Cuts at:      0     365     978 
   Size:        365     613
 
PasI CC'CwG_GG

Cuts at:      0     495     978 
   Size:        495     483
 
PpuMI rG'GwC_Cy

Cuts at:      0     321     978 
   Size:        321     657
 
PvuII CAG'CTG

Cuts at:      0     525     978 
   Size:        525     453
 
SacI G_AGCT'C

Cuts at:      0     533     978 
   Size:        533     445
 
SexAI A'CCwGG_T

Cuts at:      0     575     978 
   Size:        575     403
 
StyI C'CwwG_G

Cuts at:      0     365     978 
   Size:        365     613
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     616     678     978 
   Size:        616      62     300

  Fragments arranged by size: 

                 616     300      62
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     305     337     978 
   Size:        305      32     641

  Fragments arranged by size: 

                 641     305      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     640     978 
   Size:        640     338
 
XmnI GAAnn'nnTTC

Cuts at:      0     848     978 
   Size:        848     130
 

 Enzymes that do cut:

   Acc65I     AcuI     ApoI     BanI    BanII    BglII     BpmI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspHI    BstYI   Bsu36I     BtgI    BtgZI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRV    Hin4I  HindIII     KpnI
     MslI   MspA1I     NcoI     PasI    PpuMI    PvuII     SacI    SexAI
     StyI    TaqII     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI    EcoNI
    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     XbaI     XcmI     XhoI     XmaI     ZraI