Restriction map for
pFN21AE6639
(Linear) (Six-base) MAPSORT of: KIEE6639.seq Check: 656 from: 1 to: 750
KIEE6639.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 750
Size: 418 332
AleI CACnn'nnGTG
Cuts at: 0 548 750
Size: 548 202
AvaI C'yCGr_G
Cuts at: 0 488 750
Size: 488 262
BanII G_rGCy'C
Cuts at: 0 188 584 750
Size: 188 396 166
Fragments arranged by size:
396 188 166
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 560 750
Size: 410 150 190
Fragments arranged by size:
410 190 150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 317 750
Size: 283 34 433
Fragments arranged by size:
433 283 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 625 750
Size: 172 453 125
Fragments arranged by size:
453 172 125
Bpu10I CC'TnA_GC
Cuts at: 0 667 750
Size: 667 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 87 583 750
Size: 87 496 167
Fragments arranged by size:
496 167 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 750
Size: 19 30 701
Fragments arranged by size:
701 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 97 244 247 250 376 379 393
Size: 43 54 147 3 3 126 3 14
Cuts at: 393 750
Size: 357
Fragments arranged by size:
357 147 126 54 43 14 3 3
3
BseYI C'CCAG_C
Cuts at: 0 709 717 750
Size: 709 8 33
Fragments arranged by size:
709 33 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 326 580 750
Size: 296 30 254 170
Fragments arranged by size:
296 254 170 30
BsiHKAI G_wGCw'C
Cuts at: 0 188 584 750
Size: 188 396 166
Fragments arranged by size:
396 188 166
BsmBI CGTCTCn'nnnn_
Cuts at: 0 513 750
Size: 513 237
Bsp1286I G_dGCh'C
Cuts at: 0 188 584 750
Size: 188 396 166
Fragments arranged by size:
396 188 166
BsrBI CCG'CTC
Cuts at: 0 286 531 750
Size: 286 245 219
Fragments arranged by size:
286 245 219
BstYI r'GATC_y
Cuts at: 0 116 750
Size: 116 634
BtgI C'CryG_G
Cuts at: 0 422 750
Size: 422 328
BtsI GCAGTG_nn'
Cuts at: 0 104 750
Size: 104 646
ClaI AT'CG_AT
Cuts at: 0 539 750
Size: 539 211
EaeI y'GGCC_r
Cuts at: 0 449 750
Size: 449 301
EarI CTCTTCn'nnn_
Cuts at: 0 41 59 74 460 750
Size: 41 18 15 386 290
Fragments arranged by size:
386 290 41 18 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 355 750
Size: 355 395
EcoICRI GAG'CTC
Cuts at: 0 186 582 750
Size: 186 396 168
Fragments arranged by size:
396 186 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 418 625 750
Size: 172 246 207 125
Fragments arranged by size:
246 207 172 125
EcoNI CCTnn'n_nnAGG
Cuts at: 0 451 750
Size: 451 299
EcoO109I rG'GnC_Cy
Cuts at: 0 192 713 750
Size: 192 521 37
Fragments arranged by size:
521 192 37
HaeII r_GCGC'y
Cuts at: 0 273 327 744 750
Size: 273 54 417 6
Fragments arranged by size:
417 273 54 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 86 118 750
Size: 86 32 632
Fragments arranged by size:
632 86 32
MscI TGG'CCA
Cuts at: 0 451 750
Size: 451 299
MslI CAynn'nnrTG
Cuts at: 0 548 750
Size: 548 202
MspA1I CmG'CkG
Cuts at: 0 603 750
Size: 603 147
NcoI C'CATG_G
Cuts at: 0 422 750
Size: 422 328
PpuMI rG'GwC_Cy
Cuts at: 0 713 750
Size: 713 37
PvuII CAG'CTG
Cuts at: 0 603 750
Size: 603 147
SacI G_AGCT'C
Cuts at: 0 188 584 750
Size: 188 396 166
Fragments arranged by size:
396 188 166
SanDI GG'GwC_CC
Cuts at: 0 713 750
Size: 713 37
SfcI C'TryA_G
Cuts at: 0 435 750
Size: 435 315
SmaI CCC'GGG
Cuts at: 0 490 750
Size: 490 260
StyI C'CwwG_G
Cuts at: 0 422 452 546 575 722 750
Size: 422 30 94 29 147 28
Fragments arranged by size:
422 147 94 30 29 28
XmaI C'CCGG_G
Cuts at: 0 488 750
Size: 488 262
Enzymes that do cut:
AcuI AleI AvaI BanII BbsI BcgI BpmI Bpu10I
BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BsrBI BstYI BtgI BtsI ClaI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI
MspA1I NcoI PpuMI PvuII SacI SanDI SfcI SmaI
StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI