Restriction map for
pFN21AE6637
(Linear) (Six-base) MAPSORT of: KIEE6637.seq Check: 903 from: 1 to: 645
KIEE6637.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 645
Size: 217 428
AlwNI CAG_nnn'CTG
Cuts at: 0 27 152 242 645
Size: 27 125 90 403
Fragments arranged by size:
403 125 90 27
BanI G'GyrC_C
Cuts at: 0 211 645
Size: 211 434
BanII G_rGCy'C
Cuts at: 0 598 645
Size: 598 47
BbeI G_GCGC'C
Cuts at: 0 215 645
Size: 215 430
BclI T'GATC_A
Cuts at: 0 108 645
Size: 108 537
BglII A'GATC_T
Cuts at: 0 419 645
Size: 419 226
BlpI GC'TnA_GC
Cuts at: 0 14 645
Size: 14 631
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 612 645
Size: 612 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 315 645
Size: 283 32 330
Fragments arranged by size:
330 283 32
BsaBI GATnn'nnATC
Cuts at: 0 100 379 645
Size: 100 279 266
Fragments arranged by size:
279 266 100
BsaHI Gr'CG_yC
Cuts at: 0 212 408 645
Size: 212 196 237
Fragments arranged by size:
237 212 196
BsaWI w'CCGG_w
Cuts at: 0 188 645
Size: 188 457
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 288 318 421 451 645
Size: 288 30 103 30 194
Fragments arranged by size:
288 194 103 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 284 581 645
Size: 284 297 64
Fragments arranged by size:
297 284 64
BseYI C'CCAG_C
Cuts at: 0 284 645
Size: 284 361
BsmBI CGTCTCn'nnnn_
Cuts at: 0 331 645
Size: 331 314
Bsp1286I G_dGCh'C
Cuts at: 0 598 645
Size: 598 47
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 520 645
Size: 51 469 125
Fragments arranged by size:
469 125 51
BstYI r'GATC_y
Cuts at: 0 419 617 645
Size: 419 198 28
Fragments arranged by size:
419 198 28
BtgI C'CryG_G
Cuts at: 0 88 449 645
Size: 88 361 196
Fragments arranged by size:
361 196 88
ClaI AT'CG_AT
Cuts at: 0 625 645
Size: 625 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 328 645
Size: 328 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 283 315 645
Size: 217 66 32 330
Fragments arranged by size:
330 217 66 32
EcoO109I rG'GnC_Cy
Cuts at: 0 77 497 645
Size: 77 420 148
Fragments arranged by size:
420 148 77
EcoRV GAT'ATC
Cuts at: 0 526 645
Size: 526 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 414 645
Size: 414 231
HaeII r_GCGC'y
Cuts at: 0 215 645
Size: 215 430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 645
Size: 421 32 192
Fragments arranged by size:
421 192 32
KasI G'GCGC_C
Cuts at: 0 211 645
Size: 211 434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 645
Size: 64 581
MslI CAynn'nnrTG
Cuts at: 0 49 645
Size: 49 596
NarI GG'CG_CC
Cuts at: 0 212 645
Size: 212 433
NcoI C'CATG_G
Cuts at: 0 88 449 645
Size: 88 361 196
Fragments arranged by size:
361 196 88
PasI CC'CwG_GG
Cuts at: 0 81 645
Size: 81 564
PflMI CCAn_nnn'nTGG
Cuts at: 0 455 645
Size: 455 190
PfoI T'CCnGG_A
Cuts at: 0 260 519 645
Size: 260 259 126
Fragments arranged by size:
260 259 126
PpuMI rG'GwC_Cy
Cuts at: 0 77 645
Size: 77 568
SanDI GG'GwC_CC
Cuts at: 0 77 645
Size: 77 568
SfoI GGC'GCC
Cuts at: 0 213 645
Size: 213 432
StyI C'CwwG_G
Cuts at: 0 88 449 645
Size: 88 361 196
Fragments arranged by size:
361 196 88
XmnI GAAnn'nnTTC
Cuts at: 0 35 645
Size: 35 610
Enzymes that do cut:
AcuI AlwNI BanI BanII BbeI BclI BglII BlpI
BplI BpmI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsmBI Bsp1286I BstXI BstYI BtgI ClaI EciI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I KasI MmeI MslI
NarI NcoI PasI PflMI PfoI PpuMI SanDI SfoI
StyI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BfrBI BglI Bme1580I BmgBI BmrI
BmtI Bpu10I BpuEI BsaI BsaAI BsgI BsiEI BsiHKAI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EcoICRI EcoNI EcoRI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI