Restriction map for
pFN21AE6631
(Linear) (Six-base) MAPSORT of: KIEE6631.seq Check: 6172 from: 1 to: 948
KIEE6631.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 349 475 948
Size: 349 126 473
Fragments arranged by size:
473 349 126
AccI GT'mk_AC
Cuts at: 0 483 948
Size: 483 465
Acc65I G'GTAC_C
Cuts at: 0 14 948
Size: 14 934
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 948
Size: 410 538
AleI CACnn'nnGTG
Cuts at: 0 82 193 723 883 948
Size: 82 111 530 160 65
Fragments arranged by size:
530 160 111 82 65
ApaI G_GGCC'C
Cuts at: 0 334 948
Size: 334 614
ApaLI G'TGCA_C
Cuts at: 0 168 948
Size: 168 780
ApoI r'AATT_y
Cuts at: 0 560 754 948
Size: 560 194 194
Fragments arranged by size:
560 194 194
AseI AT'TA_AT
Cuts at: 0 440 948
Size: 440 508
AvaI C'yCGr_G
Cuts at: 0 191 575 948
Size: 191 384 373
Fragments arranged by size:
384 373 191
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 680 713 948
Size: 680 33 235
Fragments arranged by size:
680 235 33
BamHI G'GATC_C
Cuts at: 0 252 948
Size: 252 696
BanI G'GyrC_C
Cuts at: 0 14 245 948
Size: 14 231 703
Fragments arranged by size:
703 231 14
BanII G_rGCy'C
Cuts at: 0 334 948
Size: 334 614
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 948
Size: 551 397
BbvCI CC'TCA_GC
Cuts at: 0 735 747 948
Size: 735 12 201
Fragments arranged by size:
735 201 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 620 948
Size: 586 34 328
Fragments arranged by size:
586 328 34
Bme1580I G_kGCm'C
Cuts at: 0 89 172 334 948
Size: 89 83 162 614
Fragments arranged by size:
614 162 89 83
BmgBI CAC'GTC
Cuts at: 0 618 948
Size: 618 330
Bpu10I CC'TnA_GC
Cuts at: 0 735 747 948
Size: 735 12 201
Fragments arranged by size:
735 201 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 74 104 948
Size: 74 30 844
Fragments arranged by size:
844 74 30
BseYI C'CCAG_C
Cuts at: 0 229 900 948
Size: 229 671 48
Fragments arranged by size:
671 229 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 621 948
Size: 370 251 327
Fragments arranged by size:
370 327 251
BsiHKAI G_wGCw'C
Cuts at: 0 172 947 948
Size: 172 775 1
Fragments arranged by size:
775 172 1
Bsp1286I G_dGCh'C
Cuts at: 0 89 172 334 947 948
Size: 89 83 162 613 1
Fragments arranged by size:
613 162 89 83 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 349 475 948
Size: 349 126 473
Fragments arranged by size:
473 349 126
BssSI C'ACGA_G
Cuts at: 0 40 948
Size: 40 908
BstEII G'GTnAC_C
Cuts at: 0 186 648 948
Size: 186 462 300
Fragments arranged by size:
462 300 186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 618 900 948
Size: 123 495 282 48
Fragments arranged by size:
495 282 123 48
BstYI r'GATC_y
Cuts at: 0 252 302 948
Size: 252 50 646
Fragments arranged by size:
646 252 50
Bsu36I CC'TnA_GG
Cuts at: 0 163 948
Size: 163 785
BtgI C'CryG_G
Cuts at: 0 629 701 948
Size: 629 72 247
Fragments arranged by size:
629 247 72
BtsI GCAGTG_nn'
Cuts at: 0 609 718 730 948
Size: 609 109 12 218
Fragments arranged by size:
609 218 109 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 184 948
Size: 184 764
EaeI y'GGCC_r
Cuts at: 0 620 948
Size: 620 328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 948
Size: 410 538
EcoO109I rG'GnC_Cy
Cuts at: 0 330 331 948
Size: 330 1 617
Fragments arranged by size:
617 330 1
EcoRI G'AATT_C
Cuts at: 0 754 948
Size: 754 194
EcoRV GAT'ATC
Cuts at: 0 432 948
Size: 432 516
FspI TGC'GCA
Cuts at: 0 94 948
Size: 94 854
HaeII r_GCGC'y
Cuts at: 0 158 948
Size: 158 790
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 632 664 812 844 948
Size: 632 32 148 32 104
Fragments arranged by size:
632 148 104 32 32
KpnI G_GTAC'C
Cuts at: 0 18 948
Size: 18 930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 948
Size: 917 31
MslI CAynn'nnrTG
Cuts at: 0 82 193 723 866 883 911 948
Size: 82 111 530 143 17 28 37
Fragments arranged by size:
530 143 111 82 37 28 17
MspA1I CmG'CkG
Cuts at: 0 98 948
Size: 98 850
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 948
Size: 312 636
PspOMI G'GGCC_C
Cuts at: 0 330 948
Size: 330 618
PstI C_TGCA'G
Cuts at: 0 725 948
Size: 725 223
RsrII CG'GwC_CG
Cuts at: 0 138 948
Size: 138 810
SfcI C'TryA_G
Cuts at: 0 721 948
Size: 721 227
SmaI CCC'GGG
Cuts at: 0 193 577 948
Size: 193 384 371
Fragments arranged by size:
384 371 193
SspI AAT'ATT
Cuts at: 0 456 948
Size: 456 492
StyI C'CwwG_G
Cuts at: 0 683 948
Size: 683 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 295 380 948
Size: 295 85 568
Fragments arranged by size:
568 295 85
TatI w'GTAC_w
Cuts at: 0 290 948
Size: 290 658
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 74 106 583 615 948
Size: 74 32 477 32 333
Fragments arranged by size:
477 333 74 32 32
XmaI C'CCGG_G
Cuts at: 0 191 575 948
Size: 191 384 373
Fragments arranged by size:
384 373 191
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI ApaI ApaLI ApoI
AseI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI Bme1580I BmgBI Bpu10I BsaXI BseYI BsgI BsiHKAI
Bsp1286I BspMI BssSI BstEII BstXI BstYI Bsu36I BtgI
BtsI DrdI EaeI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I KpnI MmeI MslI MspA1I PflMI PspOMI
PstI RsrII SfcI SmaI SspI StyI TaqII TatI
TstI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI AlwNI AscI AsiSI AvrII BbeI BciVI BclI
BfrBI BglI BglII BlpI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI
DraI DraIII EagI EarI EciI EcoICRI EcoNI FalI
FseI FspAI HincII HindIII HpaI KasI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XbaI XcmI XhoI
XmnI ZraI