Restriction map for
pFN21AE6627
(Linear) (Six-base) MAPSORT of: KIEE6627.seq Check: 6073 from: 1 to: 660
KIEE6627.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
Acc65I G'GTAC_C
Cuts at: 0 440 608 660
Size: 440 168 52
Fragments arranged by size:
440 168 52
ApaLI G'TGCA_C
Cuts at: 0 94 660
Size: 94 566
BamHI G'GATC_C
Cuts at: 0 203 660
Size: 203 457
BanI G'GyrC_C
Cuts at: 0 4 440 554 608 621 660
Size: 4 436 114 54 13 39
Fragments arranged by size:
436 114 54 39 13 4
BbeI G_GCGC'C
Cuts at: 0 8 625 660
Size: 8 617 35
Fragments arranged by size:
617 35 8
BclI T'GATC_A
Cuts at: 0 131 660
Size: 131 529
Bme1580I G_kGCm'C
Cuts at: 0 98 395 557 660
Size: 98 297 162 103
Fragments arranged by size:
297 162 103 98
BmrI ACTGGGnnnn_n'
Cuts at: 0 537 660
Size: 537 123
Bpu10I CC'TnA_GC
Cuts at: 0 406 660
Size: 406 254
BsaI GGTCTCn'nnnn_
Cuts at: 0 303 660
Size: 303 357
BsaBI GATnn'nnATC
Cuts at: 0 367 660
Size: 367 293
BsaHI Gr'CG_yC
Cuts at: 0 5 74 622 660
Size: 5 69 548 38
Fragments arranged by size:
548 69 38 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 484 514 660
Size: 484 30 146
Fragments arranged by size:
484 146 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 270 489 660
Size: 270 219 171
Fragments arranged by size:
270 219 171
BsiEI CG_ry'CG
Cuts at: 0 71 660
Size: 71 589
BsiHKAI G_wGCw'C
Cuts at: 0 98 412 660
Size: 98 314 248
Fragments arranged by size:
314 248 98
Bsp1286I G_dGCh'C
Cuts at: 0 98 395 412 557 660
Size: 98 297 17 145 103
Fragments arranged by size:
297 145 103 98 17
BsrDI GCAATG_nn'
Cuts at: 0 123 332 660
Size: 123 209 328
Fragments arranged by size:
328 209 123
BstYI r'GATC_y
Cuts at: 0 203 660
Size: 203 457
BtgI C'CryG_G
Cuts at: 0 8 612 660
Size: 8 604 48
Fragments arranged by size:
604 48 8
BtsI GCAGTG_nn'
Cuts at: 0 293 660
Size: 293 367
EaeI y'GGCC_r
Cuts at: 0 68 660
Size: 68 592
EagI C'GGCC_G
Cuts at: 0 68 660
Size: 68 592
EarI CTCTTCn'nnn_
Cuts at: 0 418 660
Size: 418 242
EcoO109I rG'GnC_Cy
Cuts at: 0 241 654 660
Size: 241 413 6
Fragments arranged by size:
413 241 6
HaeII r_GCGC'y
Cuts at: 0 8 34 625 660
Size: 8 26 591 35
Fragments arranged by size:
591 35 26 8
KasI G'GCGC_C
Cuts at: 0 4 621 660
Size: 4 617 39
Fragments arranged by size:
617 39 4
KpnI G_GTAC'C
Cuts at: 0 444 612 660
Size: 444 168 48
Fragments arranged by size:
444 168 48
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 660
Size: 291 369
MspA1I CmG'CkG
Cuts at: 0 10 83 614 660
Size: 10 73 531 46
Fragments arranged by size:
531 73 46 10
NarI GG'CG_CC
Cuts at: 0 5 622 660
Size: 5 617 38
Fragments arranged by size:
617 38 5
PasI CC'CwG_GG
Cuts at: 0 395 660
Size: 395 265
PpuMI rG'GwC_Cy
Cuts at: 0 654 660
Size: 654 6
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 424 456 660
Size: 424 32 204
Fragments arranged by size:
424 204 32
PstI C_TGCA'G
Cuts at: 0 82 660
Size: 82 578
PvuII CAG'CTG
Cuts at: 0 83 660
Size: 83 577
SacII CC_GC'GG
Cuts at: 0 11 615 660
Size: 11 604 45
Fragments arranged by size:
604 45 11
SapI GCTCTTCn'nnn_
Cuts at: 0 418 660
Size: 418 242
SfcI C'TryA_G
Cuts at: 0 78 660
Size: 78 582
SfoI GGC'GCC
Cuts at: 0 6 623 660
Size: 6 617 37
Fragments arranged by size:
617 37 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 660
Size: 430 230
Tth111I GACn'n_nGTC
Cuts at: 0 529 660
Size: 529 131
Enzymes that do cut:
Acc65I ApaLI BamHI BanI BbeI BclI Bme1580I BmrI
Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BsiEI BsiHKAI
Bsp1286I BsrDI BstYI BtgI BtsI EaeI EagI EarI
EcoO109I HaeII KasI KpnI MmeI MspA1I NarI PasI
PpuMI PsrI PstI PvuII SacII SapI SfcI SfoI
TaqII Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BanII BbsI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BpmI BpuEI BsaAI BsaWI BseYI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI MfeI MluI MscI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacI
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TatI TstI XbaI XcmI XhoI XmaI
XmnI ZraI