(Linear) (Six-base) MAPSORT of: KIEE6625.seq  Check: 5821  from: 1  to: 1002



KIEE6625.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AatII G_ACGT'C

Cuts at:      0       9    1002 
   Size:          9     993
 
AccI GT'mk_AC

Cuts at:      0      63    1002 
   Size:         63     939
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44      71     148    1002 
   Size:         44      27      77     854

  Fragments arranged by size: 

                 854      77      44      27
 
AflIII A'CryG_T

Cuts at:      0     385    1002 
   Size:        385     617
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     931     963    1002 
   Size:        931      32      39

  Fragments arranged by size: 

                 931      39      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      22     820    1002 
   Size:         22     798     182

  Fragments arranged by size: 

                 798     182      22
 
ApoI r'AATT_y

Cuts at:      0     909    1002 
   Size:        909      93
 
AseI AT'TA_AT

Cuts at:      0     958    1002 
   Size:        958      44
 
AvaI C'yCGr_G

Cuts at:      0      75     398    1002 
   Size:         75     323     604

  Fragments arranged by size: 

                 604     323      75
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     198     656    1002 
   Size:        198     458     346

  Fragments arranged by size: 

                 458     346     198
 
BbvCI CC'TCA_GC

Cuts at:      0     935    1002 
   Size:        935      67
 
BglII A'GATC_T

Cuts at:      0     145     159    1002 
   Size:        145      14     843

  Fragments arranged by size: 

                 843     145      14
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     824    1002 
   Size:        824     178
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     216    1002 
   Size:        216     786
 
Bpu10I CC'TnA_GC

Cuts at:      0     935    1002 
   Size:        935      67
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     401    1002 
   Size:        401     601
 
BsaHI Gr'CG_yC

Cuts at:      0       6    1002 
   Size:          6     996
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     323     353    1002 
   Size:        323      30     649

  Fragments arranged by size: 

                 649     323      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      63    1002 
   Size:         63     939
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     343    1002 
   Size:        343     659
 
BstYI r'GATC_y

Cuts at:      0     145     159     849    1002 
   Size:        145      14     690     153

  Fragments arranged by size: 

                 690     153     145      14
 
BtsI GCAGTG_nn'

Cuts at:      0     366    1002 
   Size:        366     636
 
ClaI AT'CG_AT

Cuts at:      0     204    1002 
   Size:        204     798
 
EarI CTCTTCn'nnn_

Cuts at:      0     225    1002 
   Size:        225     777
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44      71     148     216    1002 
   Size:         44      27      77      68     786

  Fragments arranged by size: 

                 786      77      68      44      27
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     731    1002 
   Size:        731     271
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     323     355    1002 
   Size:        323      32     647

  Fragments arranged by size: 

                 647     323      32
 
MluI A'CGCG_T

Cuts at:      0     385    1002 
   Size:        385     617
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     993    1002 
   Size:        993       9
 
MslI CAynn'nnrTG

Cuts at:      0     182    1002 
   Size:        182     820
 
MspA1I CmG'CkG

Cuts at:      0     538     564    1002 
   Size:        538      26     438

  Fragments arranged by size: 

                 538     438      26
 
PshAI GACnn'nnGTC

Cuts at:      0       9    1002 
   Size:          9     993
 
PspXI vC'TCGA_Gb

Cuts at:      0      75    1002 
   Size:         75     927
 
PvuII CAG'CTG

Cuts at:      0     538     564    1002 
   Size:        538      26     438

  Fragments arranged by size: 

                 538     438      26
 
SapI GCTCTTCn'nnn_

Cuts at:      0     225    1002 
   Size:        225     777
 
ScaI AGT'ACT

Cuts at:      0     834    1002 
   Size:        834     168
 
SfcI C'TryA_G

Cuts at:      0     279     559    1002 
   Size:        279     280     443

  Fragments arranged by size: 

                 443     280     279
 
SmlI C'TyrA_G

Cuts at:      0      75    1002 
   Size:         75     927
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     383     843    1002 
   Size:        383     460     159

  Fragments arranged by size: 

                 460     383     159
 
TatI w'GTAC_w

Cuts at:      0     832    1002 
   Size:        832     170
 
Tth111I GACn'n_nGTC

Cuts at:      0     335    1002 
   Size:        335     667
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     561    1002 
   Size:        561     441
 
XhoI C'TCGA_G

Cuts at:      0      75    1002 
   Size:         75     927
 
XmnI GAAnn'nnTTC

Cuts at:      0     255    1002 
   Size:        255     747
 
ZraI GAC'GTC

Cuts at:      0       7    1002 
   Size:          7     995
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     AloI    AlwNI     ApoI     AseI
     AvaI     BbsI    BbvCI    BglII     BmrI     BpmI   Bpu10I     BsaI
    BsaHI    BsaXI    BseRI    BsmBI    BstYI     BtsI     ClaI     EarI
  Eco57MI     FalI    Hin4I     MluI     MmeI     MslI   MspA1I    PshAI
    PspXI    PvuII     SapI     ScaI     SfcI     SmlI    TaqII     TatI
  Tth111I     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI     XbaI     XmaI