Restriction map for
pFN21AE6621
(Linear) (Six-base) MAPSORT of: KIEE6621.seq Check: 6857 from: 1 to: 993
KIEE6621.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
Acc65I G'GTAC_C
Cuts at: 0 101 993
Size: 101 892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 993
Size: 248 745
AflIII A'CryG_T
Cuts at: 0 135 909 993
Size: 135 774 84
Fragments arranged by size:
774 135 84
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 317 349 993
Size: 317 32 644
Fragments arranged by size:
644 317 32
AlwNI CAG_nnn'CTG
Cuts at: 0 541 993
Size: 541 452
ApoI r'AATT_y
Cuts at: 0 822 993
Size: 822 171
BanI G'GyrC_C
Cuts at: 0 101 747 993
Size: 101 646 246
Fragments arranged by size:
646 246 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 380 538 860 993
Size: 380 158 322 133
Fragments arranged by size:
380 322 158 133
BbvCI CC'TCA_GC
Cuts at: 0 106 993
Size: 106 887
BciVI GTATCCnnnnn_n'
Cuts at: 0 695 993
Size: 695 298
BglI GCCn_nnn'nGGC
Cuts at: 0 283 993
Size: 283 710
BmgBI CAC'GTC
Cuts at: 0 910 993
Size: 910 83
Bpu10I CC'TnA_GC
Cuts at: 0 106 970 993
Size: 106 864 23
Fragments arranged by size:
864 106 23
BsaAI yAC'GTr
Cuts at: 0 525 777 993
Size: 525 252 216
Fragments arranged by size:
525 252 216
BsmI GAATG_Cn'
Cuts at: 0 207 484 993
Size: 207 277 509
Fragments arranged by size:
509 277 207
BsmBI CGTCTCn'nnnn_
Cuts at: 0 900 993
Size: 900 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 951 993
Size: 951 42
BstAPI GCAn_nnn'nTGC
Cuts at: 0 966 993
Size: 966 27
BstEII G'GTnAC_C
Cuts at: 0 249 993
Size: 249 744
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 993
Size: 744 249
BstYI r'GATC_y
Cuts at: 0 46 755 812 993
Size: 46 709 57 181
Fragments arranged by size:
709 181 57 46
BtgI C'CryG_G
Cuts at: 0 924 993
Size: 924 69
BtsI GCAGTG_nn'
Cuts at: 0 720 993
Size: 720 273
DraI TTT'AAA
Cuts at: 0 584 993
Size: 584 409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 993
Size: 248 745
HincII GTy'rAC
Cuts at: 0 901 993
Size: 901 92
KpnI G_GTAC'C
Cuts at: 0 105 993
Size: 105 888
MluI A'CGCG_T
Cuts at: 0 135 993
Size: 135 858
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 993
Size: 456 537
MslI CAynn'nnrTG
Cuts at: 0 122 247 489 890 993
Size: 122 125 242 401 103
Fragments arranged by size:
401 242 125 122 103
NcoI C'CATG_G
Cuts at: 0 924 993
Size: 924 69
NspI r_CATG'y
Cuts at: 0 986 993
Size: 986 7
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 133 165 993
Size: 133 32 828
Fragments arranged by size:
828 133 32
PstI C_TGCA'G
Cuts at: 0 715 993
Size: 715 278
SfcI C'TryA_G
Cuts at: 0 642 711 993
Size: 642 69 282
Fragments arranged by size:
642 282 69
SnaBI TAC'GTA
Cuts at: 0 525 993
Size: 525 468
StyI C'CwwG_G
Cuts at: 0 751 924 938 993
Size: 751 173 14 55
Fragments arranged by size:
751 173 55 14
TatI w'GTAC_w
Cuts at: 0 149 993
Size: 149 844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 603 635 993
Size: 603 32 358
Fragments arranged by size:
603 358 32
XmnI GAAnn'nnTTC
Cuts at: 0 410 607 993
Size: 410 197 386
Fragments arranged by size:
410 386 197
Enzymes that do cut:
Acc65I AcuI AflIII AloI AlwNI ApoI BanI BbsI
BbvCI BciVI BglI BmgBI Bpu10I BsaAI BsmI BsmBI
BspMI BstAPI BstEII BstXI BstYI BtgI BtsI DraI
Eco57MI HincII KpnI MluI MmeI MslI NcoI NspI
PsrI PstI SfcI SnaBI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BcgI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HindIII HpaI KasI MfeI MscI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI