Restriction map for
pFN21AE6620
(Linear) (Six-base) MAPSORT of: KIEE6620.seq Check: 8827 from: 1 to: 981
KIEE6620.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 981
Size: 883 98
AlwNI CAG_nnn'CTG
Cuts at: 0 801 924 981
Size: 801 123 57
Fragments arranged by size:
801 123 57
AvaI C'yCGr_G
Cuts at: 0 268 981
Size: 268 713
BamHI G'GATC_C
Cuts at: 0 851 981
Size: 851 130
BanI G'GyrC_C
Cuts at: 0 193 981
Size: 193 788
BanII G_rGCy'C
Cuts at: 0 229 744 931 981
Size: 229 515 187 50
Fragments arranged by size:
515 229 187 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 14 981
Size: 14 967
BbvCI CC'TCA_GC
Cuts at: 0 935 981
Size: 935 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 752 786 981
Size: 752 34 195
Fragments arranged by size:
752 195 34
BlpI GC'TnA_GC
Cuts at: 0 40 925 981
Size: 40 885 56
Fragments arranged by size:
885 56 40
Bme1580I G_kGCm'C
Cuts at: 0 196 981
Size: 196 785
BmrI ACTGGGnnnn_n'
Cuts at: 0 199 981
Size: 199 782
BmtI G_CTAG'C
Cuts at: 0 322 981
Size: 322 659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 778 981
Size: 190 588 203
Fragments arranged by size:
588 203 190
Bpu10I CC'TnA_GC
Cuts at: 0 743 935 981
Size: 743 192 46
Fragments arranged by size:
743 192 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 981
Size: 329 652
BsaHI Gr'CG_yC
Cuts at: 0 713 981
Size: 713 268
BsaWI w'CCGG_w
Cuts at: 0 328 981
Size: 328 653
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 751 781 981
Size: 751 30 200
Fragments arranged by size:
751 200 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 952 955 981
Size: 187 765 3 26
Fragments arranged by size:
765 187 26 3
BseYI C'CCAG_C
Cuts at: 0 3 510 560 981
Size: 3 507 50 421
Fragments arranged by size:
507 421 50 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 756 981
Size: 166 590 225
Fragments arranged by size:
590 225 166
Bsp1286I G_dGCh'C
Cuts at: 0 196 229 744 931 981
Size: 196 33 515 187 50
Fragments arranged by size:
515 196 187 50 33
BspEI T'CCGG_A
Cuts at: 0 328 981
Size: 328 653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 539 981
Size: 539 442
BsrDI GCAATG_nn'
Cuts at: 0 101 787 981
Size: 101 686 194
Fragments arranged by size:
686 194 101
BsrGI T'GTAC_A
Cuts at: 0 500 981
Size: 500 481
BstEII G'GTnAC_C
Cuts at: 0 405 834 981
Size: 405 429 147
Fragments arranged by size:
429 405 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 63 735 981
Size: 63 672 246
Fragments arranged by size:
672 246 63
BstYI r'GATC_y
Cuts at: 0 851 981
Size: 851 130
Bsu36I CC'TnA_GG
Cuts at: 0 78 981
Size: 78 903
BtgI C'CryG_G
Cuts at: 0 398 981
Size: 398 583
BtsI GCAGTG_nn'
Cuts at: 0 137 143 264 444 551 946 981
Size: 137 6 121 180 107 395 35
Fragments arranged by size:
395 180 137 121 107 35 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 473 508 981
Size: 473 35 473
Fragments arranged by size:
473 473 35
EaeI y'GGCC_r
Cuts at: 0 395 426 683 725 981
Size: 395 31 257 42 256
Fragments arranged by size:
395 257 256 42 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 778 883 981
Size: 190 588 105 98
Fragments arranged by size:
588 190 105 98
EcoO109I rG'GnC_Cy
Cuts at: 0 7 898 981
Size: 7 891 83
Fragments arranged by size:
891 83 7
FspI TGC'GCA
Cuts at: 0 952 981
Size: 952 29
HaeII r_GCGC'y
Cuts at: 0 267 846 981
Size: 267 579 135
Fragments arranged by size:
579 267 135
MscI TGG'CCA
Cuts at: 0 685 727 981
Size: 685 42 254
Fragments arranged by size:
685 254 42
MslI CAynn'nnrTG
Cuts at: 0 403 981
Size: 403 578
MspA1I CmG'CkG
Cuts at: 0 593 756 934 981
Size: 593 163 178 47
Fragments arranged by size:
593 178 163 47
NcoI C'CATG_G
Cuts at: 0 398 981
Size: 398 583
NheI G'CTAG_C
Cuts at: 0 318 981
Size: 318 663
PasI CC'CwG_GG
Cuts at: 0 856 981
Size: 856 125
PfoI T'CCnGG_A
Cuts at: 0 55 981
Size: 55 926
PpuMI rG'GwC_Cy
Cuts at: 0 7 981
Size: 7 974
PspXI vC'TCGA_Gb
Cuts at: 0 268 981
Size: 268 713
PstI C_TGCA'G
Cuts at: 0 150 806 981
Size: 150 656 175
Fragments arranged by size:
656 175 150
PvuII CAG'CTG
Cuts at: 0 756 981
Size: 756 225
SanDI GG'GwC_CC
Cuts at: 0 7 981
Size: 7 974
SfcI C'TryA_G
Cuts at: 0 146 802 981
Size: 146 656 179
Fragments arranged by size:
656 179 146
SmlI C'TyrA_G
Cuts at: 0 268 344 981
Size: 268 76 637
Fragments arranged by size:
637 268 76
SspI AAT'ATT
Cuts at: 0 573 981
Size: 573 408
StyI C'CwwG_G
Cuts at: 0 398 464 695 981
Size: 398 66 231 286
Fragments arranged by size:
398 286 231 66
TatI w'GTAC_w
Cuts at: 0 500 981
Size: 500 481
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 751 783 981
Size: 751 32 198
Fragments arranged by size:
751 198 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 150 229 522 693 981
Size: 150 79 293 171 288
Fragments arranged by size:
293 288 171 150 79
XhoI C'TCGA_G
Cuts at: 0 268 981
Size: 268 713
Enzymes that do cut:
AcuI AlwNI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI
BsaHI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspEI
BspMI BsrDI BsrGI BstEII BstXI BstYI Bsu36I BtgI
BtsI CspCI EaeI Eco57MI EcoO109I FspI HaeII MscI
MslI MspA1I NcoI NheI PasI PfoI PpuMI PspXI
PstI PvuII SanDI SfcI SmlI SspI StyI TatI
TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI
BfrBI BglI BglII BmgBI BplI BsaI BsaAI BsaBI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII Tth111I XbaI XmaI XmnI
ZraI