(Linear) (Six-base) MAPSORT of: KIEE6619.seq  Check: 7888  from: 1  to: 813



KIEE6619.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AccI GT'mk_AC

Cuts at:      0      47     813 
   Size:         47     766
 
Acc65I G'GTAC_C

Cuts at:      0     501     813 
   Size:        501     312
 
AflIII A'CryG_T

Cuts at:      0      58     813 
   Size:         58     755
 
ApoI r'AATT_y

Cuts at:      0     523     813 
   Size:        523     290
 
AvaI C'yCGr_G

Cuts at:      0     807     813 
   Size:        807       6
 
BanI G'GyrC_C

Cuts at:      0     501     813 
   Size:        501     312
 
BbvCI CC'TCA_GC

Cuts at:      0     271     813 
   Size:        271     542
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     690     813 
   Size:        690     123
 
Bme1580I G_kGCm'C

Cuts at:      0     769     813 
   Size:        769      44
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     422     813 
   Size:        422     391
 
Bpu10I CC'TnA_GC

Cuts at:      0     271     813 
   Size:        271     542
 
BsaAI yAC'GTr

Cuts at:      0      21     813 
   Size:         21     792
 
Bsp1286I G_dGCh'C

Cuts at:      0     769     813 
   Size:        769      44
 
BsrDI GCAATG_nn'

Cuts at:      0     441     813 
   Size:        441     372
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     500     535     813 
   Size:        500      35     278

  Fragments arranged by size: 

                 500     278      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     553     585     813 
   Size:        553      32     228

  Fragments arranged by size: 

                 553     228      32
 
HincII GTy'rAC

Cuts at:      0     603     813 
   Size:        603     210
 
KpnI G_GTAC'C

Cuts at:      0     505     813 
   Size:        505     308
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     813 
   Size:        401     412
 
MslI CAynn'nnrTG

Cuts at:      0     434     813 
   Size:        434     379
 
NdeI CA'TA_TG

Cuts at:      0     133     813 
   Size:        133     680
 
NspI r_CATG'y

Cuts at:      0      62     813 
   Size:         62     751
 
PasI CC'CwG_GG

Cuts at:      0     305     813 
   Size:        305     508
 
PciI A'CATG_T

Cuts at:      0      58     813 
   Size:         58     755
 
PshAI GACnn'nnGTC

Cuts at:      0     174     813 
   Size:        174     639
 
PsiI TTA'TAA

Cuts at:      0     746     813 
   Size:        746      67
 
PstI C_TGCA'G

Cuts at:      0      38     813 
   Size:         38     775
 
SfcI C'TryA_G

Cuts at:      0      34     813 
   Size:         34     779
 
TatI w'GTAC_w

Cuts at:      0     736     813 
   Size:        736      77
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     432     464     813 
   Size:        432      32     349

  Fragments arranged by size: 

                 432     349      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      48     813 
   Size:         48     765
 

 Enzymes that do cut:

     AccI   Acc65I   AflIII     ApoI     AvaI     BanI    BbvCI    BciVI
 Bme1580I     BmrI   Bpu10I    BsaAI Bsp1286I    BsrDI    CspCI    Hin4I
   HincII     KpnI     MmeI     MslI     NdeI     NspI     PasI     PciI
    PshAI     PsiI     PstI     SfcI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI