Restriction map for
pFN21AE6618
(Linear) (Six-base) MAPSORT of: KIEE6618.seq Check: 1849 from: 1 to: 891
KIEE6618.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AatII G_ACGT'C
Cuts at: 0 224 891
Size: 224 667
AclI AA'CG_TT
Cuts at: 0 683 891
Size: 683 208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 891
Size: 683 208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 364 891
Size: 163 201 527
Fragments arranged by size:
527 201 163
AlwNI CAG_nnn'CTG
Cuts at: 0 643 891
Size: 643 248
ApaLI G'TGCA_C
Cuts at: 0 767 891
Size: 767 124
ApoI r'AATT_y
Cuts at: 0 691 891
Size: 691 200
AvaI C'yCGr_G
Cuts at: 0 64 109 116 891
Size: 64 45 7 775
Fragments arranged by size:
775 64 45 7
BanI G'GyrC_C
Cuts at: 0 127 891
Size: 127 764
BanII G_rGCy'C
Cuts at: 0 116 537 812 891
Size: 116 421 275 79
Fragments arranged by size:
421 275 116 79
BbvCI CC'TCA_GC
Cuts at: 0 644 891
Size: 644 247
BglI GCCn_nnn'nGGC
Cuts at: 0 431 891
Size: 431 460
BlpI GC'TnA_GC
Cuts at: 0 806 891
Size: 806 85
Bme1580I G_kGCm'C
Cuts at: 0 179 618 771 891
Size: 179 439 153 120
Fragments arranged by size:
439 179 153 120
BmgBI CAC'GTC
Cuts at: 0 873 891
Size: 873 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 891
Size: 640 251
Bpu10I CC'TnA_GC
Cuts at: 0 25 296 644 891
Size: 25 271 348 247
Fragments arranged by size:
348 271 247 25
BsaBI GATnn'nnATC
Cuts at: 0 628 891
Size: 628 263
BsaHI Gr'CG_yC
Cuts at: 0 221 891
Size: 221 670
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 71 530 891
Size: 71 459 361
Fragments arranged by size:
459 361 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 700 817 891
Size: 133 567 117 74
Fragments arranged by size:
567 133 117 74
BsiHKAI G_wGCw'C
Cuts at: 0 302 537 771 891
Size: 302 235 234 120
Fragments arranged by size:
302 235 234 120
Bsp1286I G_dGCh'C
Cuts at: 0 116 179 302 537 618 771 812 891
Size: 116 63 123 235 81 153 41 79
Fragments arranged by size:
235 153 123 116 81 79 63 41
BsrBI CCG'CTC
Cuts at: 0 133 891
Size: 133 758
BssSI C'ACGA_G
Cuts at: 0 56 856 891
Size: 56 800 35
Fragments arranged by size:
800 56 35
BtgI C'CryG_G
Cuts at: 0 158 891
Size: 158 733
BtsI GCAGTG_nn'
Cuts at: 0 278 712 891
Size: 278 434 179
Fragments arranged by size:
434 278 179
DraI TTT'AAA
Cuts at: 0 690 891
Size: 690 201
DraIII CAC_nnn'GTG
Cuts at: 0 723 891
Size: 723 168
EaeI y'GGCC_r
Cuts at: 0 314 891
Size: 314 577
EarI CTCTTCn'nnn_
Cuts at: 0 441 714 891
Size: 441 273 177
Fragments arranged by size:
441 273 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 253 891
Size: 253 638
EcoICRI GAG'CTC
Cuts at: 0 535 891
Size: 535 356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 683 891
Size: 640 43 208
Fragments arranged by size:
640 208 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 820 891
Size: 820 71
EcoO109I rG'GnC_Cy
Cuts at: 0 61 286 424 891
Size: 61 225 138 467
Fragments arranged by size:
467 225 138 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 442 474 891
Size: 442 32 417
Fragments arranged by size:
442 417 32
HincII GTy'rAC
Cuts at: 0 359 891
Size: 359 532
MscI TGG'CCA
Cuts at: 0 316 891
Size: 316 575
MslI CAynn'nnrTG
Cuts at: 0 860 875 891
Size: 860 15 16
Fragments arranged by size:
860 16 15
MspA1I CmG'CkG
Cuts at: 0 37 160 801 891
Size: 37 123 641 90
Fragments arranged by size:
641 123 90 37
NdeI CA'TA_TG
Cuts at: 0 347 891
Size: 347 544
PasI CC'CwG_GG
Cuts at: 0 744 891
Size: 744 147
PvuII CAG'CTG
Cuts at: 0 801 891
Size: 801 90
SacI G_AGCT'C
Cuts at: 0 537 891
Size: 537 354
SacII CC_GC'GG
Cuts at: 0 161 891
Size: 161 730
SapI GCTCTTCn'nnn_
Cuts at: 0 441 891
Size: 441 450
SfcI C'TryA_G
Cuts at: 0 527 891
Size: 527 364
SmaI CCC'GGG
Cuts at: 0 66 118 891
Size: 66 52 773
Fragments arranged by size:
773 66 52
StyI C'CwwG_G
Cuts at: 0 392 891
Size: 392 499
XmaI C'CCGG_G
Cuts at: 0 64 116 891
Size: 64 52 775
Fragments arranged by size:
775 64 52
ZraI GAC'GTC
Cuts at: 0 222 891
Size: 222 669
Enzymes that do cut:
AatII AclI AcuI AlfI AlwNI ApaLI ApoI AvaI
BanI BanII BbvCI BglI BlpI Bme1580I BmgBI BpmI
Bpu10I BsaBI BsaHI BseRI BsgI BsiHKAI Bsp1286I BsrBI
BssSI BtgI BtsI DraI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII MscI MslI
MspA1I NdeI PasI PvuII SacI SacII SapI SfcI
SmaI StyI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglII
BmrI BmtI BplI BpuEI BsaI BsaAI BsaWI BsaXI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI