Restriction map for
pFN21AE6617
(Linear) (Six-base) MAPSORT of: KIEE6617.seq Check: 2800 from: 1 to: 1008
KIEE6617.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1008
Size: 78 930
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 529 561 1008
Size: 529 32 447
Fragments arranged by size:
529 447 32
AlwNI CAG_nnn'CTG
Cuts at: 0 243 563 915 1008
Size: 243 320 352 93
Fragments arranged by size:
352 320 243 93
BamHI G'GATC_C
Cuts at: 0 188 893 1008
Size: 188 705 115
Fragments arranged by size:
705 188 115
BanI G'GyrC_C
Cuts at: 0 824 1008
Size: 824 184
BanII G_rGCy'C
Cuts at: 0 573 1008
Size: 573 435
BbeI G_GCGC'C
Cuts at: 0 828 1008
Size: 828 180
BglI GCCn_nnn'nGGC
Cuts at: 0 64 978 1008
Size: 64 914 30
Fragments arranged by size:
914 64 30
BlpI GC'TnA_GC
Cuts at: 0 256 697 1008
Size: 256 441 311
Fragments arranged by size:
441 311 256
BmgBI CAC'GTC
Cuts at: 0 759 927 1008
Size: 759 168 81
Fragments arranged by size:
759 168 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 573 1008
Size: 573 435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 286 388 517 540 892 1008
Size: 94 192 102 129 23 352 116
Fragments arranged by size:
352 192 129 116 102 94 23
Bpu10I CC'TnA_GC
Cuts at: 0 769 1008
Size: 769 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 433 643 1008
Size: 433 210 365
Fragments arranged by size:
433 365 210
BsaAI yAC'GTr
Cuts at: 0 42 498 1008
Size: 42 456 510
Fragments arranged by size:
510 456 42
BsaHI Gr'CG_yC
Cuts at: 0 825 1008
Size: 825 183
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 865 895 1008
Size: 865 30 113
Fragments arranged by size:
865 113 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 625 1008
Size: 625 383
BseYI C'CCAG_C
Cuts at: 0 68 233 575 1008
Size: 68 165 342 433
Fragments arranged by size:
433 342 165 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 673 1008
Size: 535 138 335
Fragments arranged by size:
535 335 138
BsiEI CG_ry'CG
Cuts at: 0 59 682 876 1008
Size: 59 623 194 132
Fragments arranged by size:
623 194 132 59
BsiHKAI G_wGCw'C
Cuts at: 0 573 1008
Size: 573 435
Bsp1286I G_dGCh'C
Cuts at: 0 573 1008
Size: 573 435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 100 110 173 860 1008
Size: 100 10 63 687 148
Fragments arranged by size:
687 148 100 63 10
BsrBI CCG'CTC
Cuts at: 0 810 957 1008
Size: 810 147 51
Fragments arranged by size:
810 147 51
BsrDI GCAATG_nn'
Cuts at: 0 671 1008
Size: 671 337
BsrGI T'GTAC_A
Cuts at: 0 668 1008
Size: 668 340
BssSI C'ACGA_G
Cuts at: 0 130 607 1008
Size: 130 477 401
Fragments arranged by size:
477 401 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 243 1008
Size: 243 765
BstEII G'GTnAC_C
Cuts at: 0 463 1008
Size: 463 545
BstXI CCAn_nnnn'nTGG
Cuts at: 0 475 636 931 1008
Size: 475 161 295 77
Fragments arranged by size:
475 295 161 77
BstYI r'GATC_y
Cuts at: 0 188 893 1008
Size: 188 705 115
Fragments arranged by size:
705 188 115
BtgI C'CryG_G
Cuts at: 0 62 1008
Size: 62 946
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 321 639 1008
Size: 321 318 369
Fragments arranged by size:
369 321 318
BtsI GCAGTG_nn'
Cuts at: 0 89 1008
Size: 89 919
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 500 535 1008
Size: 500 35 473
Fragments arranged by size:
500 473 35
EaeI y'GGCC_r
Cuts at: 0 56 353 679 917 1008
Size: 56 297 326 238 91
Fragments arranged by size:
326 297 238 91 56
EagI C'GGCC_G
Cuts at: 0 56 679 1008
Size: 56 623 329
Fragments arranged by size:
623 329 56
EarI CTCTTCn'nnn_
Cuts at: 0 904 1008
Size: 904 104
EcoICRI GAG'CTC
Cuts at: 0 571 1008
Size: 571 437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 94 286 388 517 540 892 1008
Size: 78 16 192 102 129 23 352 116
Fragments arranged by size:
352 192 129 116 102 78 23 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 196 1008
Size: 196 812
EcoO109I rG'GnC_Cy
Cuts at: 0 792 1008
Size: 792 216
HaeII r_GCGC'y
Cuts at: 0 828 1008
Size: 828 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 865 897 1008
Size: 865 32 111
Fragments arranged by size:
865 111 32
KasI G'GCGC_C
Cuts at: 0 824 1008
Size: 824 184
MscI TGG'CCA
Cuts at: 0 355 1008
Size: 355 653
MslI CAynn'nnrTG
Cuts at: 0 289 1008
Size: 289 719
MspA1I CmG'CkG
Cuts at: 0 72 326 351 582 684 1008
Size: 72 254 25 231 102 324
Fragments arranged by size:
324 254 231 102 72 25
NarI GG'CG_CC
Cuts at: 0 825 1008
Size: 825 183
PasI CC'CwG_GG
Cuts at: 0 636 1008
Size: 636 372
PflMI CCAn_nnn'nTGG
Cuts at: 0 308 563 915 1008
Size: 308 255 352 93
Fragments arranged by size:
352 308 255 93
PstI C_TGCA'G
Cuts at: 0 647 1008
Size: 647 361
PvuII CAG'CTG
Cuts at: 0 72 351 1008
Size: 72 279 657
Fragments arranged by size:
657 279 72
SacI G_AGCT'C
Cuts at: 0 573 1008
Size: 573 435
SfcI C'TryA_G
Cuts at: 0 643 996 1008
Size: 643 353 12
Fragments arranged by size:
643 353 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 64 1008
Size: 64 944
SfoI GGC'GCC
Cuts at: 0 826 1008
Size: 826 182
StyI C'CwwG_G
Cuts at: 0 302 356 1008
Size: 302 54 652
Fragments arranged by size:
652 302 54
TatI w'GTAC_w
Cuts at: 0 149 363 668 1008
Size: 149 214 305 340
Fragments arranged by size:
340 305 214 149
Tth111I GACn'n_nGTC
Cuts at: 0 925 1008
Size: 925 83
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 1008
Size: 69 939
Enzymes that do cut:
AcuI AloI AlwNI BamHI BanI BanII BbeI BglI
BlpI BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI
MspA1I NarI PasI PflMI PstI PvuII SacI SfcI
SfiI SfoI StyI TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII Bme1580I BmtI BplI BpuEI
BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrFI
BssHII BstBI BstZ17I Bsu36I ClaI DraI DraIII DrdI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI XbaI XhoI XmaI XmnI ZraI