Restriction map for
pFN21AE6615
(Linear) (Six-base) MAPSORT of: KIEE6615.seq Check: 4781 from: 1 to: 867
KIEE6615.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
Acc65I G'GTAC_C
Cuts at: 0 797 867
Size: 797 70
AhdI GACnn_n'nnGTC
Cuts at: 0 304 867
Size: 304 563
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 175 867
Size: 175 692
ApoI r'AATT_y
Cuts at: 0 274 867
Size: 274 593
AvaI C'yCGr_G
Cuts at: 0 378 867
Size: 378 489
BanI G'GyrC_C
Cuts at: 0 797 819 867
Size: 797 22 48
Fragments arranged by size:
797 48 22
BanII G_rGCy'C
Cuts at: 0 390 401 867
Size: 390 11 466
Fragments arranged by size:
466 390 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 143 867
Size: 143 724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 427 461 867
Size: 427 34 406
Fragments arranged by size:
427 406 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 549 867
Size: 549 318
BmgBI CAC'GTC
Cuts at: 0 432 867
Size: 432 435
BmtI G_CTAG'C
Cuts at: 0 676 867
Size: 676 191
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 404 867
Size: 404 463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 814 867
Size: 502 312 53
Fragments arranged by size:
502 312 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 272 867
Size: 272 595
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 699 729 867
Size: 699 30 138
Fragments arranged by size:
699 138 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 484 707 867
Size: 475 9 223 160
Fragments arranged by size:
475 223 160 9
BseYI C'CCAG_C
Cuts at: 0 503 802 867
Size: 503 299 65
Fragments arranged by size:
503 299 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 867
Size: 145 722
BsiHKAI G_wGCw'C
Cuts at: 0 749 867
Size: 749 118
BsmI GAATG_Cn'
Cuts at: 0 693 867
Size: 693 174
BsmBI CGTCTCn'nnnn_
Cuts at: 0 291 867
Size: 291 576
Bsp1286I G_dGCh'C
Cuts at: 0 390 401 749 867
Size: 390 11 348 118
Fragments arranged by size:
390 348 118 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 682 867
Size: 167 515 185
Fragments arranged by size:
515 185 167
BstBI TT'CG_AA
Cuts at: 0 447 867
Size: 447 420
BstYI r'GATC_y
Cuts at: 0 53 867
Size: 53 814
BtgI C'CryG_G
Cuts at: 0 791 815 867
Size: 791 24 52
Fragments arranged by size:
791 52 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 484 639 867
Size: 484 155 228
Fragments arranged by size:
484 228 155
BtsI GCAGTG_nn'
Cuts at: 0 159 867
Size: 159 708
DraI TTT'AAA
Cuts at: 0 60 867
Size: 60 807
EarI CTCTTCn'nnn_
Cuts at: 0 461 867
Size: 461 406
EcoO109I rG'GnC_Cy
Cuts at: 0 423 867
Size: 423 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 290 322 483 515 867
Size: 290 32 161 32 352
Fragments arranged by size:
352 290 161 32 32
KpnI G_GTAC'C
Cuts at: 0 801 867
Size: 801 66
NheI G'CTAG_C
Cuts at: 0 672 867
Size: 672 195
PasI CC'CwG_GG
Cuts at: 0 125 867
Size: 125 742
PflMI CCAn_nnn'nTGG
Cuts at: 0 855 867
Size: 855 12
PpuMI rG'GwC_Cy
Cuts at: 0 423 867
Size: 423 444
PstI C_TGCA'G
Cuts at: 0 686 867
Size: 686 181
ScaI AGT'ACT
Cuts at: 0 550 867
Size: 550 317
SfcI C'TryA_G
Cuts at: 0 682 867
Size: 682 185
SmlI C'TyrA_G
Cuts at: 0 517 829 867
Size: 517 312 38
Fragments arranged by size:
517 312 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 117 867
Size: 117 750
TatI w'GTAC_w
Cuts at: 0 548 591 867
Size: 548 43 276
Fragments arranged by size:
548 276 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 699 731 867
Size: 699 32 136
Fragments arranged by size:
699 136 32
Enzymes that do cut:
Acc65I AhdI AlfI ApoI AvaI BanI BanII BbsI
BcgI BciVI BmgBI BmtI BplI BpuEI BsaI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BstBI BstYI BtgI BtgZI BtsI DraI EarI EcoO109I
Hin4I KpnI NheI PasI PflMI PpuMI PstI ScaI
SfcI SmlI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI