Restriction map for
pFN21AE6614
(Linear) (Six-base) MAPSORT of: KIEE6614.seq Check: 4099 from: 1 to: 726
KIEE6614.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AatII G_ACGT'C
Cuts at: 0 304 726
Size: 304 422
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 726
Size: 187 539
AfeI AGC'GCT
Cuts at: 0 355 726
Size: 355 371
AlwNI CAG_nnn'CTG
Cuts at: 0 591 651 702 726
Size: 591 60 51 24
Fragments arranged by size:
591 60 51 24
AvaI C'yCGr_G
Cuts at: 0 295 726
Size: 295 431
BamHI G'GATC_C
Cuts at: 0 494 726
Size: 494 232
BanII G_rGCy'C
Cuts at: 0 632 674 726
Size: 632 42 52
Fragments arranged by size:
632 52 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 286 320 726
Size: 286 34 406
Fragments arranged by size:
406 286 34
BclI T'GATC_A
Cuts at: 0 456 726
Size: 456 270
BglI GCCn_nnn'nGGC
Cuts at: 0 345 726
Size: 345 381
BlpI GC'TnA_GC
Cuts at: 0 547 631 726
Size: 547 84 95
Fragments arranged by size:
547 95 84
Bme1580I G_kGCm'C
Cuts at: 0 215 726
Size: 215 511
BmrI ACTGGGnnnn_n'
Cuts at: 0 527 726
Size: 527 199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 274 658 673 726
Size: 196 78 384 15 53
Fragments arranged by size:
384 196 78 53 15
Bpu10I CC'TnA_GC
Cuts at: 0 538 726
Size: 538 188
BsaAI yAC'GTr
Cuts at: 0 462 726
Size: 462 264
BsaHI Gr'CG_yC
Cuts at: 0 301 726
Size: 301 425
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 646 676 726
Size: 646 30 50
Fragments arranged by size:
646 50 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 655 670 694 697 726
Size: 148 507 15 24 3 29
Fragments arranged by size:
507 148 29 24 15 3
BseYI C'CCAG_C
Cuts at: 0 489 635 726
Size: 489 146 91
Fragments arranged by size:
489 146 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 726
Size: 38 688
BsiHKAI G_wGCw'C
Cuts at: 0 517 726
Size: 517 209
BsmI GAATG_Cn'
Cuts at: 0 93 726
Size: 93 633
BsmBI CGTCTCn'nnnn_
Cuts at: 0 292 726
Size: 292 434
Bsp1286I G_dGCh'C
Cuts at: 0 215 517 632 674 726
Size: 215 302 115 42 52
Fragments arranged by size:
302 215 115 52 42
BsrBI CCG'CTC
Cuts at: 0 180 726
Size: 180 546
BsrFI r'CCGG_y
Cuts at: 0 468 726
Size: 468 258
BstAPI GCAn_nnn'nTGC
Cuts at: 0 61 702 726
Size: 61 641 24
Fragments arranged by size:
641 61 24
BstYI r'GATC_y
Cuts at: 0 494 613 726
Size: 494 119 113
Fragments arranged by size:
494 119 113
BtgI C'CryG_G
Cuts at: 0 599 726
Size: 599 127
BtsI GCAGTG_nn'
Cuts at: 0 59 113 726
Size: 59 54 613
Fragments arranged by size:
613 59 54
EaeI y'GGCC_r
Cuts at: 0 257 726
Size: 257 469
EarI CTCTTCn'nnn_
Cuts at: 0 406 726
Size: 406 320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 196 274 658 673 726
Size: 187 9 78 384 15 53
Fragments arranged by size:
384 187 78 53 15 9
EcoO109I rG'GnC_Cy
Cuts at: 0 242 726
Size: 242 484
HaeII r_GCGC'y
Cuts at: 0 357 525 726
Size: 357 168 201
Fragments arranged by size:
357 201 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 726
Size: 646 32 48
Fragments arranged by size:
646 48 32
HincII GTy'rAC
Cuts at: 0 723 726
Size: 723 3
MscI TGG'CCA
Cuts at: 0 259 726
Size: 259 467
MspA1I CmG'CkG
Cuts at: 0 484 635 726
Size: 484 151 91
Fragments arranged by size:
484 151 91
NaeI GCC'GGC
Cuts at: 0 470 726
Size: 470 256
NcoI C'CATG_G
Cuts at: 0 599 726
Size: 599 127
NgoMIV G'CCGG_C
Cuts at: 0 468 726
Size: 468 258
PflMI CCAn_nnn'nTGG
Cuts at: 0 255 726
Size: 255 471
PfoI T'CCnGG_A
Cuts at: 0 248 674 726
Size: 248 426 52
Fragments arranged by size:
426 248 52
PmlI CAC'GTG
Cuts at: 0 462 726
Size: 462 264
PpuMI rG'GwC_Cy
Cuts at: 0 242 726
Size: 242 484
PstI C_TGCA'G
Cuts at: 0 50 374 698 726
Size: 50 324 324 28
Fragments arranged by size:
324 324 50 28
PvuII CAG'CTG
Cuts at: 0 635 726
Size: 635 91
SbfI CC_TGCA'GG
Cuts at: 0 698 726
Size: 698 28
SexAI A'CCwGG_T
Cuts at: 0 716 726
Size: 716 10
SfcI C'TryA_G
Cuts at: 0 46 370 694 726
Size: 46 324 324 32
Fragments arranged by size:
324 324 46 32
StyI C'CwwG_G
Cuts at: 0 385 599 726
Size: 385 214 127
Fragments arranged by size:
385 214 127
TatI w'GTAC_w
Cuts at: 0 74 726
Size: 74 652
ZraI GAC'GTC
Cuts at: 0 302 726
Size: 302 424
Enzymes that do cut:
AatII AcuI AfeI AlwNI AvaI BamHI BanII BcgI
BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BsrBI BsrFI BstAPI BstYI BtgI BtsI EaeI
EarI Eco57MI EcoO109I HaeII Hin4I HincII MscI MspA1I
NaeI NcoI NgoMIV PflMI PfoI PmlI PpuMI PstI
PvuII SbfI SexAI SfcI StyI TatI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BciVI
BfrBI BglII BmgBI BmtI BplI BpuEI BsaI BsaBI
BsaWI BsiEI BsiWI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI