(Linear) (Six-base) MAPSORT of: KIEE6613.seq  Check: 3948  from: 1  to: 756



KIEE6613.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     529     756 
   Size:        379     150     227

  Fragments arranged by size: 

                 379     227     150
 
ApaI G_GGCC'C

Cuts at:      0     498     756 
   Size:        498     258
 
BanII G_rGCy'C

Cuts at:      0     498     756 
   Size:        498     258
 
Bme1580I G_kGCm'C

Cuts at:      0     498     756 
   Size:        498     258
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     216     756 
   Size:        216     540
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     756 
   Size:        294     462
 
BsaAI yAC'GTr

Cuts at:      0     378     756 
   Size:        378     378
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      60     679     756 
   Size:         60     619      77

  Fragments arranged by size: 

                 619      77      60
 
BseYI C'CCAG_C

Cuts at:      0     108     756 
   Size:        108     648
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     756 
   Size:        484     272
 
BsiHKAI G_wGCw'C

Cuts at:      0     377     532     539     632     756 
   Size:        377     155       7      93     124

  Fragments arranged by size: 

                 377     155     124      93       7
 
Bsp1286I G_dGCh'C

Cuts at:      0     377     498     532     539     632     756 
   Size:        377     121      34       7      93     124

  Fragments arranged by size: 

                 377     124     121      93      34       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     224     756 
   Size:        224     532
 
BsrDI GCAATG_nn'

Cuts at:      0      14     470     756 
   Size:         14     456     286

  Fragments arranged by size: 

                 456     286      14
 
BstYI r'GATC_y

Cuts at:      0     585     756 
   Size:        585     171
 
Bsu36I CC'TnA_GG

Cuts at:      0     199     756 
   Size:        199     557
 
BtsI GCAGTG_nn'

Cuts at:      0     367     756 
   Size:        367     389
 
DraIII CAC_nnn'GTG

Cuts at:      0     210     756 
   Size:        210     546
 
EaeI y'GGCC_r

Cuts at:      0     380     456     756 
   Size:        380      76     300

  Fragments arranged by size: 

                 380     300      76
 
EarI CTCTTCn'nnn_

Cuts at:      0      79     628     756 
   Size:         79     549     128

  Fragments arranged by size: 

                 549     128      79
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     688     756 
   Size:        688      68
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     379     529     756 
   Size:        294      85     150     227

  Fragments arranged by size: 

                 294     227     150      85
 
EcoO109I rG'GnC_Cy

Cuts at:      0     494     495     756 
   Size:        494       1     261

  Fragments arranged by size: 

                 494     261       1
 
FspI TGC'GCA

Cuts at:      0     565     756 
   Size:        565     191
 
MscI TGG'CCA

Cuts at:      0     382     458     756 
   Size:        382      76     298

  Fragments arranged by size: 

                 382     298      76
 
MslI CAynn'nnrTG

Cuts at:      0     406     756 
   Size:        406     350
 
MspA1I CmG'CkG

Cuts at:      0     487     756 
   Size:        487     269
 
NspI r_CATG'y

Cuts at:      0     360     756 
   Size:        360     396
 
PmlI CAC'GTG

Cuts at:      0     378     756 
   Size:        378     378
 
PspOMI G'GGCC_C

Cuts at:      0     494     756 
   Size:        494     262
 
PstI C_TGCA'G

Cuts at:      0     234     756 
   Size:        234     522
 
PvuII CAG'CTG

Cuts at:      0     487     756 
   Size:        487     269
 
SfcI C'TryA_G

Cuts at:      0     230     756 
   Size:        230     526
 
SphI G_CATG'C

Cuts at:      0     360     756 
   Size:        360     396
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     198     756 
   Size:        198     558
 
TatI w'GTAC_w

Cuts at:      0     569     756 
   Size:        569     187
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27     734     756 
   Size:         27     707      22

  Fragments arranged by size: 

                 707      27      22
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     359     756 
   Size:        359     397
 

 Enzymes that do cut:

     AcuI     ApaI    BanII Bme1580I     BmrI     BpmI    BsaAI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI    BstYI   Bsu36I
     BtsI   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I     FspI
     MscI     MslI   MspA1I     NspI     PmlI   PspOMI     PstI    PvuII
     SfcI     SphI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI
     DraI     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI