Restriction map for
pFN21AE6612
(Linear) (Six-base) MAPSORT of: KIEE6612.seq Check: 36 from: 1 to: 933
KIEE6612.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 400 636 933
Size: 296 104 236 297
Fragments arranged by size:
297 296 236 104
AflIII A'CryG_T
Cuts at: 0 534 933
Size: 534 399
AleI CACnn'nnGTG
Cuts at: 0 892 933
Size: 892 41
AlwNI CAG_nnn'CTG
Cuts at: 0 260 595 923 933
Size: 260 335 328 10
Fragments arranged by size:
335 328 260 10
ApaI G_GGCC'C
Cuts at: 0 482 933
Size: 482 451
ApaLI G'TGCA_C
Cuts at: 0 568 933
Size: 568 365
BamHI G'GATC_C
Cuts at: 0 248 933
Size: 248 685
BanI G'GyrC_C
Cuts at: 0 523 933
Size: 523 410
BanII G_rGCy'C
Cuts at: 0 166 355 482 933
Size: 166 189 127 451
Fragments arranged by size:
451 189 166 127
BbvCI CC'TCA_GC
Cuts at: 0 363 933
Size: 363 570
Bme1580I G_kGCm'C
Cuts at: 0 482 572 933
Size: 482 90 361
Fragments arranged by size:
482 361 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 160 477 933
Size: 160 317 456
Fragments arranged by size:
456 317 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 390 487 528 766 933
Size: 200 190 97 41 238 167
Fragments arranged by size:
238 200 190 167 97 41
Bpu10I CC'TnA_GC
Cuts at: 0 363 933
Size: 363 570
BsaBI GATnn'nnATC
Cuts at: 0 430 933
Size: 430 503
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 173 203 543 573 933
Size: 173 30 340 30 360
Fragments arranged by size:
360 340 173 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 525 564 883 904 933
Size: 525 39 319 21 29
Fragments arranged by size:
525 319 39 29 21
BseYI C'CCAG_C
Cuts at: 0 253 933
Size: 253 680
BsiHKAI G_wGCw'C
Cuts at: 0 268 572 933
Size: 268 304 361
Fragments arranged by size:
361 304 268
Bsp1286I G_dGCh'C
Cuts at: 0 166 268 355 482 572 933
Size: 166 102 87 127 90 361
Fragments arranged by size:
361 166 127 102 90 87
BsrFI r'CCGG_y
Cuts at: 0 773 933
Size: 773 160
BsrGI T'GTAC_A
Cuts at: 0 230 933
Size: 230 703
BssSI C'ACGA_G
Cuts at: 0 234 933
Size: 234 699
BstAPI GCAn_nnn'nTGC
Cuts at: 0 263 933
Size: 263 670
BstXI CCAn_nnnn'nTGG
Cuts at: 0 696 933
Size: 696 237
BstYI r'GATC_y
Cuts at: 0 248 926 933
Size: 248 678 7
Fragments arranged by size:
678 248 7
Bsu36I CC'TnA_GG
Cuts at: 0 905 916 933
Size: 905 11 17
Fragments arranged by size:
905 17 11
BtgI C'CryG_G
Cuts at: 0 581 800 887 933
Size: 581 219 87 46
Fragments arranged by size:
581 219 87 46
EaeI y'GGCC_r
Cuts at: 0 95 311 933
Size: 95 216 622
Fragments arranged by size:
622 216 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 296 390 400 487 528 636 766
Size: 200 96 94 10 87 41 108 130
Cuts at: 766 933
Size: 167
Fragments arranged by size:
200 167 130 108 96 94 87 41
10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 507 933
Size: 507 426
EcoO109I rG'GnC_Cy
Cuts at: 0 280 471 478 479 920 933
Size: 280 191 7 1 441 13
Fragments arranged by size:
441 280 191 13 7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 168 405 933
Size: 168 237 528
Fragments arranged by size:
528 237 168
HaeII r_GCGC'y
Cuts at: 0 650 933
Size: 650 283
MscI TGG'CCA
Cuts at: 0 97 933
Size: 97 836
MslI CAynn'nnrTG
Cuts at: 0 892 933
Size: 892 41
MspA1I CmG'CkG
Cuts at: 0 260 857 933
Size: 260 597 76
Fragments arranged by size:
597 260 76
NaeI GCC'GGC
Cuts at: 0 775 933
Size: 775 158
NcoI C'CATG_G
Cuts at: 0 581 933
Size: 581 352
NgoMIV G'CCGG_C
Cuts at: 0 773 933
Size: 773 160
NspI r_CATG'y
Cuts at: 0 538 933
Size: 538 395
PciI A'CATG_T
Cuts at: 0 534 933
Size: 534 399
PflMI CCAn_nnn'nTGG
Cuts at: 0 260 933
Size: 260 673
PpuMI rG'GwC_Cy
Cuts at: 0 280 471 933
Size: 280 191 462
Fragments arranged by size:
462 280 191
PspOMI G'GGCC_C
Cuts at: 0 478 933
Size: 478 455
PstI C_TGCA'G
Cuts at: 0 862 933
Size: 862 71
PvuII CAG'CTG
Cuts at: 0 260 857 933
Size: 260 597 76
Fragments arranged by size:
597 260 76
ScaI AGT'ACT
Cuts at: 0 787 933
Size: 787 146
SexAI A'CCwGG_T
Cuts at: 0 452 933
Size: 452 481
SfcI C'TryA_G
Cuts at: 0 858 933
Size: 858 75
StyI C'CwwG_G
Cuts at: 0 581 933
Size: 581 352
TatI w'GTAC_w
Cuts at: 0 126 191 230 785 933
Size: 126 65 39 555 148
Fragments arranged by size:
555 148 126 65 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 173 205 264 296 933
Size: 173 32 59 32 637
Fragments arranged by size:
637 173 59 32 32
XbaI T'CTAG_A
Cuts at: 0 21 933
Size: 21 912
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 758 933
Size: 758 175
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI ApaLI BamHI BanI
BanII BbvCI Bme1580I BmrI BpmI Bpu10I BsaBI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BsrFI BsrGI BssSI BstAPI
BstXI BstYI Bsu36I BtgI EaeI Eco57MI EcoNI EcoO109I
FalI HaeII MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PciI PflMI PpuMI PspOMI PstI PvuII ScaI
SexAI SfcI StyI TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BssHII BstBI BstEII
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XhoI XmaI XmnI ZraI