(Linear) (Six-base) MAPSORT of: KIEE6610.seq  Check: 2701  from: 1  to: 780



KIEE6610.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AccI GT'mk_AC

Cuts at:      0     622     780 
   Size:        622     158
 
AclI AA'CG_TT

Cuts at:      0     102     780 
   Size:        102     678
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     780 
   Size:        154     626
 
AlwNI CAG_nnn'CTG

Cuts at:      0     259     780 
   Size:        259     521
 
AvaI C'yCGr_G

Cuts at:      0     290     780 
   Size:        290     490
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      37     780 
   Size:         37     743
 
BfrBI ATG'CAT

Cuts at:      0     201     780 
   Size:        201     579
 
BlpI GC'TnA_GC

Cuts at:      0     140     780 
   Size:        140     640
 
Bme1580I G_kGCm'C

Cuts at:      0     378     780 
   Size:        378     402
 
BsaAI yAC'GTr

Cuts at:      0     373     780 
   Size:        373     407
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     689     719     780 
   Size:        689      30      61

  Fragments arranged by size: 

                 689      61      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     378     780 
   Size:        378     402
 
BspHI T'CATG_A

Cuts at:      0      96     780 
   Size:         96     684
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     267     780 
   Size:        267     513
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     259     780 
   Size:        259     521
 
BstBI TT'CG_AA

Cuts at:      0     408     780 
   Size:        408     372
 
BstEII G'GTnAC_C

Cuts at:      0     729     780 
   Size:        729      51
 
BstZ17I GTA'TAC

Cuts at:      0     623     780 
   Size:        623     157
 
EarI CTCTTCn'nnn_

Cuts at:      0     433     780 
   Size:        433     347
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     780 
   Size:        154     626
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     271     303     780 
   Size:        271      32     477

  Fragments arranged by size: 

                 477     271      32
 
HindIII A'AGCT_T

Cuts at:      0     142     780 
   Size:        142     638
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     447     780 
   Size:        447     333
 
NsiI A_TGCA'T

Cuts at:      0     203     780 
   Size:        203     577
 
PasI CC'CwG_GG

Cuts at:      0     378     780 
   Size:        378     402
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     589     780 
   Size:        589     191
 
ScaI AGT'ACT

Cuts at:      0     302     780 
   Size:        302     478
 
SmlI C'TyrA_G

Cuts at:      0     290     780 
   Size:        290     490
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     622     780 
   Size:        622     158
 
TatI w'GTAC_w

Cuts at:      0     300     780 
   Size:        300     480
 
XhoI C'TCGA_G

Cuts at:      0     290     780 
   Size:        290     490
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AlwNI     AvaI    BciVI    BfrBI     BlpI
 Bme1580I    BsaAI    BsaXI Bsp1286I    BspHI    BspMI   BstAPI    BstBI
   BstEII  BstZ17I     EarI  Eco57MI    Hin4I  HindIII     MmeI     NsiI
     PasI    PflMI     ScaI     SmlI    TaqII     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI     BglI    BglII    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI