(Linear) (Six-base) MAPSORT of: KIEE6609.seq  Check: 5933  from: 1  to: 897



KIEE6609.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AccI GT'mk_AC

Cuts at:      0     531     897 
   Size:        531     366
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     897 
   Size:        508     389
 
AflIII A'CryG_T

Cuts at:      0      47     392     897 
   Size:         47     345     505

  Fragments arranged by size: 

                 505     345      47
 
AseI AT'TA_AT

Cuts at:      0     184     668     897 
   Size:        184     484     229

  Fragments arranged by size: 

                 484     229     184
 
AvrII C'CTAG_G

Cuts at:      0     324     897 
   Size:        324     573
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      91     162     897 
   Size:         91      71     735

  Fragments arranged by size: 

                 735      91      71
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      62     897 
   Size:         62     835
 
BfrBI ATG'CAT

Cuts at:      0     181     897 
   Size:        181     716
 
BlpI GC'TnA_GC

Cuts at:      0     675     897 
   Size:        675     222
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     897 
   Size:        310     587
 
BsaAI yAC'GTr

Cuts at:      0      48     897 
   Size:         48     849
 
BspHI T'CATG_A

Cuts at:      0     624     897 
   Size:        624     273
 
BsrBI CCG'CTC

Cuts at:      0     795     897 
   Size:        795     102
 
BsrDI GCAATG_nn'

Cuts at:      0     247     897 
   Size:        247     650
 
BsrGI T'GTAC_A

Cuts at:      0     590     897 
   Size:        590     307
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     496     897 
   Size:        496     401
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      62     897 
   Size:         62     835
 
BstYI r'GATC_y

Cuts at:      0       3     897 
   Size:          3     894
 
BtgI C'CryG_G

Cuts at:      0     253     897 
   Size:        253     644
 
DraI TTT'AAA

Cuts at:      0     206     897 
   Size:        206     691
 
DraIII CAC_nnn'GTG

Cuts at:      0     573     737     897 
   Size:        573     164     160

  Fragments arranged by size: 

                 573     164     160
 
EaeI y'GGCC_r

Cuts at:      0      64     897 
   Size:         64     833
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     508     897 
   Size:        310     198     389

  Fragments arranged by size: 

                 389     310     198
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     515     897 
   Size:        515     382
 
HaeII r_GCGC'y

Cuts at:      0     447     897 
   Size:        447     450
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     465     497     897 
   Size:        465      32     400

  Fragments arranged by size: 

                 465     400      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     108     897 
   Size:        108     789
 
MscI TGG'CCA

Cuts at:      0      66     897 
   Size:         66     831
 
MslI CAynn'nnrTG

Cuts at:      0     252     391     629     897 
   Size:        252     139     238     268

  Fragments arranged by size: 

                 268     252     238     139
 
NcoI C'CATG_G

Cuts at:      0     253     897 
   Size:        253     644
 
NdeI CA'TA_TG

Cuts at:      0     339     698     897 
   Size:        339     359     199

  Fragments arranged by size: 

                 359     339     199
 
NsiI A_TGCA'T

Cuts at:      0     183     897 
   Size:        183     714
 
NspI r_CATG'y

Cuts at:      0     396     897 
   Size:        396     501
 
PciI A'CATG_T

Cuts at:      0     392     897 
   Size:        392     505
 
SspI AAT'ATT

Cuts at:      0     727     897 
   Size:        727     170
 
StuI AGG'CCT

Cuts at:      0     419     897 
   Size:        419     478
 
StyI C'CwwG_G

Cuts at:      0     253     324     897 
   Size:        253      71     573

  Fragments arranged by size: 

                 573     253      71
 
TatI w'GTAC_w

Cuts at:      0       9     590     897 
   Size:          9     581     307

  Fragments arranged by size: 

                 581     307       9
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     844     897 
   Size:        844      53
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AseI    AvrII     BbsI    BciVI    BfrBI
     BlpI     BpmI    BsaAI    BspHI    BsrBI    BsrDI    BsrGI   BstAPI
    BstXI    BstYI     BtgI     DraI   DraIII     EaeI  Eco57MI     FalI
    HaeII    Hin4I     MmeI     MscI     MslI     NcoI     NdeI     NsiI
     NspI     PciI     SspI     StuI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI     BclI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrFI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI