(Linear) (Six-base) MAPSORT of: KIEE6607.seq  Check: 1838  from: 1  to: 804



KIEE6607.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AatII G_ACGT'C

Cuts at:      0     734     804 
   Size:        734      70
 
AccI GT'mk_AC

Cuts at:      0     301     804 
   Size:        301     503
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35      50     804 
   Size:         35      15     754

  Fragments arranged by size: 

                 754      35      15
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     700     732     804 
   Size:        700      32      72

  Fragments arranged by size: 

                 700      72      32
 
ApoI r'AATT_y

Cuts at:      0     455     590     804 
   Size:        455     135     214

  Fragments arranged by size: 

                 455     214     135
 
BfrBI ATG'CAT

Cuts at:      0      81     804 
   Size:         81     723
 
Bme1580I G_kGCm'C

Cuts at:      0     211     588     804 
   Size:        211     377     216

  Fragments arranged by size: 

                 377     216     211
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     804 
   Size:        263     541
 
BsaAI yAC'GTr

Cuts at:      0     189     804 
   Size:        189     615
 
BsaHI Gr'CG_yC

Cuts at:      0     731     804 
   Size:        731      73
 
BsaWI w'CCGG_w

Cuts at:      0     548     804 
   Size:        548     256
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     261     291     804 
   Size:        261      30     513

  Fragments arranged by size: 

                 513     261      30
 
BsmI GAATG_Cn'

Cuts at:      0     387     804 
   Size:        387     417
 
Bsp1286I G_dGCh'C

Cuts at:      0     211     588     804 
   Size:        211     377     216

  Fragments arranged by size: 

                 377     216     211
 
BsrDI GCAATG_nn'

Cuts at:      0     392     804 
   Size:        392     412
 
BstYI r'GATC_y

Cuts at:      0     237     804 
   Size:        237     567
 
BtsI GCAGTG_nn'

Cuts at:      0     209     804 
   Size:        209     595
 
EarI CTCTTCn'nnn_

Cuts at:      0     308     804 
   Size:        308     496
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35      50     263     804 
   Size:         35      15     213     541

  Fragments arranged by size: 

                 541     213      35      15
 
EcoRI G'AATT_C

Cuts at:      0     455     590     804 
   Size:        455     135     214

  Fragments arranged by size: 

                 455     214     135
 
EcoRV GAT'ATC

Cuts at:      0     423     804 
   Size:        423     381
 
HincII GTy'rAC

Cuts at:      0     702     804 
   Size:        702     102
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     775     804 
   Size:        775      29
 
NsiI A_TGCA'T

Cuts at:      0      83     804 
   Size:         83     721
 
PfoI T'CCnGG_A

Cuts at:      0     598     725     804 
   Size:        598     127      79

  Fragments arranged by size: 

                 598     127      79
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390     804 
   Size:        358      32     414

  Fragments arranged by size: 

                 414     358      32
 
PshAI GACnn'nnGTC

Cuts at:      0     734     804 
   Size:        734      70
 
PstI C_TGCA'G

Cuts at:      0     522     804 
   Size:        522     282
 
SfcI C'TryA_G

Cuts at:      0     518     804 
   Size:        518     286
 
StuI AGG'CCT

Cuts at:      0      40     804 
   Size:         40     764
 
StyI C'CwwG_G

Cuts at:      0     580     804 
   Size:        580     224
 
XmnI GAAnn'nnTTC

Cuts at:      0     509     594     804 
   Size:        509      85     210

  Fragments arranged by size: 

                 509     210      85
 
ZraI GAC'GTC

Cuts at:      0     732     804 
   Size:        732      72
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AloI     ApoI    BfrBI Bme1580I     BpmI
    BsaAI    BsaHI    BsaWI    BsaXI     BsmI Bsp1286I    BsrDI    BstYI
     BtsI     EarI  Eco57MI    EcoRI    EcoRV   HincII     MmeI     NsiI
     PfoI     PpiI    PshAI     PstI     SfcI     StuI     StyI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI