Restriction map for
pFN21AE6605
(Linear) (Six-base) MAPSORT of: KIEE6605.seq Check: 4856 from: 1 to: 813
KIEE6605.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AfeI AGC'GCT
Cuts at: 0 484 813
Size: 484 329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 367 813
Size: 367 446
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 813
Size: 314 32 467
Fragments arranged by size:
467 314 32
AvaI C'yCGr_G
Cuts at: 0 741 813
Size: 741 72
BanI G'GyrC_C
Cuts at: 0 157 634 813
Size: 157 477 179
Fragments arranged by size:
477 179 157
BanII G_rGCy'C
Cuts at: 0 407 698 813
Size: 407 291 115
Fragments arranged by size:
407 291 115
BbeI G_GCGC'C
Cuts at: 0 161 638 813
Size: 161 477 175
Fragments arranged by size:
477 175 161
BbsI GAAGACnn'nnnn_
Cuts at: 0 594 813
Size: 594 219
BbvCI CC'TCA_GC
Cuts at: 0 74 813
Size: 74 739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 345 379 813
Size: 345 34 434
Fragments arranged by size:
434 345 34
BglI GCCn_nnn'nGGC
Cuts at: 0 521 592 813
Size: 521 71 221
Fragments arranged by size:
521 221 71
BglII A'GATC_T
Cuts at: 0 455 813
Size: 455 358
BlpI GC'TnA_GC
Cuts at: 0 529 813
Size: 529 284
Bme1580I G_kGCm'C
Cuts at: 0 526 813
Size: 526 287
BmgBI CAC'GTC
Cuts at: 0 759 813
Size: 759 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 813
Size: 643 170
Bpu10I CC'TnA_GC
Cuts at: 0 74 813
Size: 74 739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 813
Size: 391 422
BsaI GGTCTCn'nnnn_
Cuts at: 0 390 813
Size: 390 423
BsaHI Gr'CG_yC
Cuts at: 0 92 158 494 635 813
Size: 92 66 336 141 178
Fragments arranged by size:
336 178 141 92 66
BsaWI w'CCGG_w
Cuts at: 0 289 813
Size: 289 524
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 314 344 813
Size: 314 30 469
Fragments arranged by size:
469 314 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 730 813
Size: 490 240 83
Fragments arranged by size:
490 240 83
BseYI C'CCAG_C
Cuts at: 0 497 813
Size: 497 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 363 813
Size: 124 239 450
Fragments arranged by size:
450 239 124
BsiHKAI G_wGCw'C
Cuts at: 0 407 813
Size: 407 406
BsmI GAATG_Cn'
Cuts at: 0 426 813
Size: 426 387
BsmBI CGTCTCn'nnnn_
Cuts at: 0 436 813
Size: 436 377
Bsp1286I G_dGCh'C
Cuts at: 0 407 526 698 813
Size: 407 119 172 115
Fragments arranged by size:
407 172 119 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 813
Size: 470 343
BsrFI r'CCGG_y
Cuts at: 0 78 430 586 813
Size: 78 352 156 227
Fragments arranged by size:
352 227 156 78
BstYI r'GATC_y
Cuts at: 0 107 455 813
Size: 107 348 358
Fragments arranged by size:
358 348 107
BtgI C'CryG_G
Cuts at: 0 188 516 813
Size: 188 328 297
Fragments arranged by size:
328 297 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 101 813
Size: 101 712
BtsI GCAGTG_nn'
Cuts at: 0 52 237 813
Size: 52 185 576
Fragments arranged by size:
576 185 52
DrdI GACnn_nn'nnGTC
Cuts at: 0 308 382 813
Size: 308 74 431
Fragments arranged by size:
431 308 74
EaeI y'GGCC_r
Cuts at: 0 566 813
Size: 566 247
EarI CTCTTCn'nnn_
Cuts at: 0 171 813
Size: 171 642
EcoICRI GAG'CTC
Cuts at: 0 405 813
Size: 405 408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 813
Size: 643 170
EcoNI CCTnn'n_nnAGG
Cuts at: 0 625 813
Size: 625 188
EcoO109I rG'GnC_Cy
Cuts at: 0 301 813
Size: 301 512
FspI TGC'GCA
Cuts at: 0 355 813
Size: 355 458
HaeII r_GCGC'y
Cuts at: 0 161 486 638 813
Size: 161 325 152 175
Fragments arranged by size:
325 175 161 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 82 114 813
Size: 26 56 32 699
Fragments arranged by size:
699 56 32 26
KasI G'GCGC_C
Cuts at: 0 157 634 813
Size: 157 477 179
Fragments arranged by size:
477 179 157
MslI CAynn'nnrTG
Cuts at: 0 339 775 813
Size: 339 436 38
Fragments arranged by size:
436 339 38
MspA1I CmG'CkG
Cuts at: 0 351 393 501 518 689 813
Size: 351 42 108 17 171 124
Fragments arranged by size:
351 171 124 108 42 17
NaeI GCC'GGC
Cuts at: 0 80 588 813
Size: 80 508 225
Fragments arranged by size:
508 225 80
NarI GG'CG_CC
Cuts at: 0 158 635 813
Size: 158 477 178
Fragments arranged by size:
477 178 158
NgoMIV G'CCGG_C
Cuts at: 0 78 586 813
Size: 78 508 227
Fragments arranged by size:
508 227 78
NspI r_CATG'y
Cuts at: 0 537 813
Size: 537 276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 314 346 394 426 813
Size: 314 32 48 32 387
Fragments arranged by size:
387 314 48 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 301 813
Size: 301 512
PvuII CAG'CTG
Cuts at: 0 351 393 501 813
Size: 351 42 108 312
Fragments arranged by size:
351 312 108 42
SacI G_AGCT'C
Cuts at: 0 407 813
Size: 407 406
SacII CC_GC'GG
Cuts at: 0 519 813
Size: 519 294
SexAI A'CCwGG_T
Cuts at: 0 380 813
Size: 380 433
SfoI GGC'GCC
Cuts at: 0 159 636 813
Size: 159 477 177
Fragments arranged by size:
477 177 159
SgrAI Cr'CCGG_yG
Cuts at: 0 430 813
Size: 430 383
SmlI C'TyrA_G
Cuts at: 0 406 813
Size: 406 407
SphI G_CATG'C
Cuts at: 0 537 813
Size: 537 276
StuI AGG'CCT
Cuts at: 0 329 813
Size: 329 484
StyI C'CwwG_G
Cuts at: 0 539 813
Size: 539 274
TatI w'GTAC_w
Cuts at: 0 644 779 813
Size: 644 135 34
Fragments arranged by size:
644 135 34
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 369 561 813
Size: 369 192 252
Fragments arranged by size:
369 252 192
Enzymes that do cut:
AfeI AlfI AloI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BstYI
BtgI BtgZI BtsI DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I KasI MslI MspA1I
NaeI NarI NgoMIV NspI PpiI PpuMI PvuII SacI
SacII SexAI SfoI SgrAI SmlI SphI StuI StyI
TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BmrI BmtI BplI BsaAI BsaBI BsiEI BsiWI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII
EagI EciI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SalI SanDI SapI SbfI
ScaI SfcI SfiI SgfI SmaI SnaBI SpeI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
XmnI ZraI