(Linear) (Six-base) MAPSORT of: KIEE6604.seq  Check: 5717  from: 1  to: 729



KIEE6604.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     168     729 
   Size:        168     561
 
AgeI A'CCGG_T

Cuts at:      0      12     729 
   Size:         12     717
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     246     729 
   Size:        246     483
 
ApaLI G'TGCA_C

Cuts at:      0     231     729 
   Size:        231     498
 
ApoI r'AATT_y

Cuts at:      0     722     729 
   Size:        722       7
 
BamHI G'GATC_C

Cuts at:      0      66     729 
   Size:         66     663
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     314     729 
   Size:        314     415
 
BglII A'GATC_T

Cuts at:      0     624     729 
   Size:        624     105
 
Bme1580I G_kGCm'C

Cuts at:      0     235     729 
   Size:        235     494
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      90     729 
   Size:         90     639
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     667     729 
   Size:        667      62
 
BsaWI w'CCGG_w

Cuts at:      0      12     729 
   Size:         12     717
 
BsiHKAI G_wGCw'C

Cuts at:      0     235     729 
   Size:        235     494
 
BsmI GAATG_Cn'

Cuts at:      0     213     729 
   Size:        213     516
 
Bsp1286I G_dGCh'C

Cuts at:      0     235     729 
   Size:        235     494
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      60     168     729 
   Size:         60     108     561

  Fragments arranged by size: 

                 561     108      60
 
BsrFI r'CCGG_y

Cuts at:      0      12     568     729 
   Size:         12     556     161

  Fragments arranged by size: 

                 556     161      12
 
BstYI r'GATC_y

Cuts at:      0      66     350     624     729 
   Size:         66     284     274     105

  Fragments arranged by size: 

                 284     274     105      66
 
DraIII CAC_nnn'GTG

Cuts at:      0     565     729 
   Size:        565     164
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     383     729 
   Size:        383     346
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      56     729 
   Size:         56     673
 
HincII GTy'rAC

Cuts at:      0      89     729 
   Size:         89     640
 
HpaI GTT'AAC

Cuts at:      0      89     729 
   Size:         89     640
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     381     729 
   Size:        381     348
 
MspA1I CmG'CkG

Cuts at:      0     661     729 
   Size:        661      68
 
NspI r_CATG'y

Cuts at:      0     174     729 
   Size:        174     555
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     662     694     709     729 
   Size:        662      32      15      20

  Fragments arranged by size: 

                 662      32      20      15
 
PstI C_TGCA'G

Cuts at:      0      57     729 
   Size:         57     672
 
PvuII CAG'CTG

Cuts at:      0     661     729 
   Size:        661      68
 
SexAI A'CCwGG_T

Cuts at:      0     437     729 
   Size:        437     292
 
SfcI C'TryA_G

Cuts at:      0      53     729 
   Size:         53     676
 
SmlI C'TyrA_G

Cuts at:      0     682     729 
   Size:        682      47
 
SspI AAT'ATT

Cuts at:      0     505     729 
   Size:        505     224
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     109     729 
   Size:        109     620
 
TatI w'GTAC_w

Cuts at:      0     271     729 
   Size:        271     458
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     445     729 
   Size:        445     284
 

 Enzymes that do cut:

     AarI     AgeI     AlfI    ApaLI     ApoI    BamHI     BbsI    BglII
 Bme1580I     BmrI    BpuEI    BsaWI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrFI    BstYI   DraIII     DrdI    EcoNI   HincII     HpaI     MmeI
   MspA1I     NspI     PsrI     PstI    PvuII    SexAI     SfcI     SmlI
     SspI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI