(Linear) (Six-base) MAPSORT of: KIEE6586.seq  Check: 7721  from: 1  to: 498



KIEE6586.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AvaI C'yCGr_G

Cuts at:      0      47     498 
   Size:         47     451
 
BamHI G'GATC_C

Cuts at:      0     243     498 
   Size:        243     255
 
BanII G_rGCy'C

Cuts at:      0      48     498 
   Size:         48     450
 
Bme1580I G_kGCm'C

Cuts at:      0     359     498 
   Size:        359     139
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     498 
   Size:         92     406
 
BsaAI yAC'GTr

Cuts at:      0     414     498 
   Size:        414      84
 
BsaHI Gr'CG_yC

Cuts at:      0     261     498 
   Size:        261     237
 
BsaWI w'CCGG_w

Cuts at:      0     157     498 
   Size:        157     341
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      77      80     498 
   Size:         77       3     418

  Fragments arranged by size: 

                 418      77       3
 
BsiEI CG_ry'CG

Cuts at:      0     162     498 
   Size:        162     336
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     252     498 
   Size:        252     246
 
Bsp1286I G_dGCh'C

Cuts at:      0      48     359     498 
   Size:         48     311     139

  Fragments arranged by size: 

                 311     139      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     286     498 
   Size:        286     212
 
BssSI C'ACGA_G

Cuts at:      0     302     498 
   Size:        302     196
 
BstYI r'GATC_y

Cuts at:      0     243     498 
   Size:        243     255
 
BtgI C'CryG_G

Cuts at:      0      93     498 
   Size:         93     405
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     210     498 
   Size:        210     288
 
EaeI y'GGCC_r

Cuts at:      0      36     219     408     416     498 
   Size:         36     183     189       8      82

  Fragments arranged by size: 

                 189     183      82      36       8
 
EarI CTCTTCn'nnn_

Cuts at:      0      75     498 
   Size:         75     423
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     498 
   Size:         92     406
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      62     498 
   Size:         62     436
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      33     498 
   Size:         33     465
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     429     461     498 
   Size:        429      32      37

  Fragments arranged by size: 

                 429      37      32
 
HincII GTy'rAC

Cuts at:      0     448     498 
   Size:        448      50
 
MfeI C'AATT_G

Cuts at:      0     423     498 
   Size:        423      75
 
MscI TGG'CCA

Cuts at:      0      38     410     418     498 
   Size:         38     372       8      80

  Fragments arranged by size: 

                 372      80      38       8
 
MspA1I CmG'CkG

Cuts at:      0     459     498 
   Size:        459      39
 
NcoI C'CATG_G

Cuts at:      0      93     498 
   Size:         93     405
 
PmlI CAC'GTG

Cuts at:      0     414     498 
   Size:        414      84
 
StyI C'CwwG_G

Cuts at:      0      93     483     498 
   Size:         93     390      15

  Fragments arranged by size: 

                 390      93      15
 

 Enzymes that do cut:

     AvaI    BamHI    BanII Bme1580I     BpmI    BsaAI    BsaHI    BsaWI
    BseRI    BsiEI    BsmBI Bsp1286I    BspMI    BssSI    BstYI     BtgI
    BtgZI     EaeI     EarI  Eco57MI    EcoNI     FalI    Hin4I   HincII
     MfeI     MscI   MspA1I     NcoI     PmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI