(Linear) (Six-base) MAPSORT of: KIEE6581.seq  Check: 3278  from: 1  to: 396



KIEE6581.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AccI GT'mk_AC

Cuts at:      0      26     396 
   Size:         26     370
 
AfeI AGC'GCT

Cuts at:      0     162     396 
   Size:        162     234
 
ApoI r'AATT_y

Cuts at:      0     139     277     396 
   Size:        139     138     119

  Fragments arranged by size: 

                 139     138     119
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     396 
   Size:        267     129
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     396 
   Size:        274     122
 
BsaWI w'CCGG_w

Cuts at:      0      29     396 
   Size:         29     367
 
BspHI T'CATG_A

Cuts at:      0      99     396 
   Size:         99     297
 
BstBI TT'CG_AA

Cuts at:      0     168     396 
   Size:        168     228
 
BstYI r'GATC_y

Cuts at:      0     380     396 
   Size:        380      16
 
DraI TTT'AAA

Cuts at:      0     387     396 
   Size:        387       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     396 
   Size:        267     129
 
EcoO109I rG'GnC_Cy

Cuts at:      0     242     396 
   Size:        242     154
 
HaeII r_GCGC'y

Cuts at:      0     164     396 
   Size:        164     232
 
HindIII A'AGCT_T

Cuts at:      0      88     396 
   Size:         88     308
 
PpuMI rG'GwC_Cy

Cuts at:      0     242     396 
   Size:        242     154
 
SmlI C'TyrA_G

Cuts at:      0     253     396 
   Size:        253     143
 
SpeI A'CTAG_T

Cuts at:      0     339     396 
   Size:        339      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55     396 
   Size:         55     341
 

 Enzymes that do cut:

     AccI     AfeI     ApoI     BpmI    BpuEI    BsaWI    BspHI    BstBI
    BstYI     DraI  Eco57MI EcoO109I    HaeII  HindIII    PpuMI     SmlI
     SpeI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI