(Linear) (Six-base) MAPSORT of: KIEE6578.seq  Check: 8501  from: 1  to: 312



KIEE6578.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AflII C'TTAA_G

Cuts at:      0      97     312 
   Size:         97     215
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     112     144     312 
   Size:        112      32     168

  Fragments arranged by size: 

                 168     112      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      61     312 
   Size:         61     251
 
AvaI C'yCGr_G

Cuts at:      0     184     312 
   Size:        184     128
 
BanI G'GyrC_C

Cuts at:      0      92     312 
   Size:         92     220
 
Bme1580I G_kGCm'C

Cuts at:      0      49     312 
   Size:         49     263
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      59     312 
   Size:         59     253
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     112     142     312 
   Size:        112      30     170

  Fragments arranged by size: 

                 170     112      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      10      97     133     312 
   Size:         10      87      36     179

  Fragments arranged by size: 

                 179      87      36      10
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     312 
   Size:         49     263
 
EaeI y'GGCC_r

Cuts at:      0     138     312 
   Size:        138     174
 
EarI CTCTTCn'nnn_

Cuts at:      0     281     312 
   Size:        281      31
 
HindIII A'AGCT_T

Cuts at:      0     266     312 
   Size:        266      46
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     312 
   Size:        173     139
 
MscI TGG'CCA

Cuts at:      0     140     312 
   Size:        140     172
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144     312 
   Size:        112      32     168

  Fragments arranged by size: 

                 168     112      32
 
PstI C_TGCA'G

Cuts at:      0      81     312 
   Size:         81     231
 
SapI GCTCTTCn'nnn_

Cuts at:      0     281     312 
   Size:        281      31
 
SfcI C'TryA_G

Cuts at:      0      77     312 
   Size:         77     235
 
SmaI CCC'GGG

Cuts at:      0     186     312 
   Size:        186     126
 
SmlI C'TyrA_G

Cuts at:      0      97     312 
   Size:         97     215
 
StyI C'CwwG_G

Cuts at:      0     248     312 
   Size:        248      64
 
XmaI C'CCGG_G

Cuts at:      0     184     312 
   Size:        184     128
 

 Enzymes that do cut:

    AflII     AloI    AlwNI     AvaI     BanI Bme1580I     BmrI    BsaXI
    BseRI Bsp1286I     EaeI     EarI  HindIII     MmeI     MscI     PpiI
     PstI     SapI     SfcI     SmaI     SmlI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI