(Linear) (Six-base) MAPSORT of: KIEE6577.seq  Check: 3191  from: 1  to: 213



KIEE6577.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AleI CACnn'nnGTG

Cuts at:      0     133     213 
   Size:        133      80
 
BanI G'GyrC_C

Cuts at:      0     116     213 
   Size:        116      97
 
BanII G_rGCy'C

Cuts at:      0     208     213 
   Size:        208       5
 
BbeI G_GCGC'C

Cuts at:      0     120     213 
   Size:        120      93
 
BlpI GC'TnA_GC

Cuts at:      0     175     213 
   Size:        175      38
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     137     213 
   Size:        137      76
 
Bpu10I CC'TnA_GC

Cuts at:      0     149     213 
   Size:        149      64
 
BsaHI Gr'CG_yC

Cuts at:      0     117     213 
   Size:        117      96
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     213 
   Size:         22     191
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     213 
   Size:        109     104
 
Bsp1286I G_dGCh'C

Cuts at:      0     208     213 
   Size:        208       5
 
BsrDI GCAATG_nn'

Cuts at:      0     142     213 
   Size:        142      71
 
DraI TTT'AAA

Cuts at:      0      51     213 
   Size:         51     162
 
DraIII CAC_nnn'GTG

Cuts at:      0     132     213 
   Size:        132      81
 
HaeII r_GCGC'y

Cuts at:      0     120     213 
   Size:        120      93
 
KasI G'GCGC_C

Cuts at:      0     116     213 
   Size:        116      97
 
MslI CAynn'nnrTG

Cuts at:      0     133     213 
   Size:        133      80
 
NarI GG'CG_CC

Cuts at:      0     117     213 
   Size:        117      96
 
SfoI GGC'GCC

Cuts at:      0     118     213 
   Size:        118      95
 

 Enzymes that do cut:

     AleI     BanI    BanII     BbeI     BlpI     BplI   Bpu10I    BsaHI
    BseRI     BsgI Bsp1286I    BsrDI     DraI   DraIII    HaeII     KasI
     MslI     NarI     SfoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI