(Linear) (Six-base) MAPSORT of: KIEE6576.seq  Check: 8715  from: 1  to: 321



KIEE6576.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     293     305     308     321 
   Size:        293      12       3      13

  Fragments arranged by size: 

                 293      13      12       3
 
BanI G'GyrC_C

Cuts at:      0      91     184     321 
   Size:         91      93     137

  Fragments arranged by size: 

                 137      93      91
 
Bme1580I G_kGCm'C

Cuts at:      0      96     321 
   Size:         96     225
 
BsiHKAI G_wGCw'C

Cuts at:      0      40     321 
   Size:         40     281
 
Bsp1286I G_dGCh'C

Cuts at:      0      40      96     321 
   Size:         40      56     225

  Fragments arranged by size: 

                 225      56      40
 
BspHI T'CATG_A

Cuts at:      0      23     321 
   Size:         23     298
 
BsrFI r'CCGG_y

Cuts at:      0     213     321 
   Size:        213     108
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     305     321 
   Size:        305      16
 
BstYI r'GATC_y

Cuts at:      0     224     321 
   Size:        224      97
 
BtgI C'CryG_G

Cuts at:      0     110     321 
   Size:        110     211
 
EaeI y'GGCC_r

Cuts at:      0     310     321 
   Size:        310      11
 
EcoO109I rG'GnC_Cy

Cuts at:      0     290     321 
   Size:        290      31
 
MscI TGG'CCA

Cuts at:      0     312     321 
   Size:        312       9
 
MspA1I CmG'CkG

Cuts at:      0     305     321 
   Size:        305      16
 
NcoI C'CATG_G

Cuts at:      0     110     321 
   Size:        110     211
 
PstI C_TGCA'G

Cuts at:      0     298     321 
   Size:        298      23
 
PvuII CAG'CTG

Cuts at:      0     305     321 
   Size:        305      16
 
SfcI C'TryA_G

Cuts at:      0     294     321 
   Size:        294      27
 
StyI C'CwwG_G

Cuts at:      0     110     321 
   Size:        110     211
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     183     215     321 
   Size:        183      32     106

  Fragments arranged by size: 

                 183     106      32
 

 Enzymes that do cut:

    AlwNI     BanI Bme1580I  BsiHKAI Bsp1286I    BspHI    BsrFI   BstAPI
    BstYI     BtgI     EaeI EcoO109I     MscI   MspA1I     NcoI     PstI
    PvuII     SfcI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI