Restriction map for
pFN21AE6575
(Linear) (Six-base) MAPSORT of: KIEE6575.seq Check: 5244 from: 1 to: 585
KIEE6575.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AgeI A'CCGG_T
Cuts at: 0 166 585
Size: 166 419
AleI CACnn'nnGTG
Cuts at: 0 169 585
Size: 169 416
AlwNI CAG_nnn'CTG
Cuts at: 0 309 585
Size: 309 276
ApoI r'AATT_y
Cuts at: 0 562 585
Size: 562 23
AvaI C'yCGr_G
Cuts at: 0 325 585
Size: 325 260
BanI G'GyrC_C
Cuts at: 0 570 585
Size: 570 15
BanII G_rGCy'C
Cuts at: 0 61 326 585
Size: 61 265 259
Fragments arranged by size:
265 259 61
BbeI G_GCGC'C
Cuts at: 0 574 585
Size: 574 11
BbvCI CC'TCA_GC
Cuts at: 0 217 585
Size: 217 368
BmgBI CAC'GTC
Cuts at: 0 516 585
Size: 516 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 397 570 585
Size: 97 300 173 15
Fragments arranged by size:
300 173 97 15
Bpu10I CC'TnA_GC
Cuts at: 0 217 585
Size: 217 368
BsaHI Gr'CG_yC
Cuts at: 0 571 585
Size: 571 14
BsaWI w'CCGG_w
Cuts at: 0 166 585
Size: 166 419
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 585
Size: 412 173
BseYI C'CCAG_C
Cuts at: 0 344 585
Size: 344 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 352 585
Size: 147 205 233
Fragments arranged by size:
233 205 147
BsmBI CGTCTCn'nnnn_
Cuts at: 0 73 585
Size: 73 512
Bsp1286I G_dGCh'C
Cuts at: 0 61 326 585
Size: 61 265 259
Fragments arranged by size:
265 259 61
BsrBI CCG'CTC
Cuts at: 0 231 356 522 585
Size: 231 125 166 63
Fragments arranged by size:
231 166 125 63
BsrDI GCAATG_nn'
Cuts at: 0 150 585
Size: 150 435
BsrFI r'CCGG_y
Cuts at: 0 166 585
Size: 166 419
BssHII G'CGCG_C
Cuts at: 0 467 469 585
Size: 467 2 116
Fragments arranged by size:
467 116 2
BstYI r'GATC_y
Cuts at: 0 260 585
Size: 260 325
Bsu36I CC'TnA_GG
Cuts at: 0 292 585
Size: 292 293
BtgI C'CryG_G
Cuts at: 0 438 585
Size: 438 147
BtsI GCAGTG_nn'
Cuts at: 0 538 585
Size: 538 47
EaeI y'GGCC_r
Cuts at: 0 188 449 585
Size: 188 261 136
Fragments arranged by size:
261 188 136
EarI CTCTTCn'nnn_
Cuts at: 0 355 389 585
Size: 355 34 196
Fragments arranged by size:
355 196 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 328 585
Size: 328 257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 397 570 585
Size: 97 300 173 15
Fragments arranged by size:
300 173 97 15
EcoO109I rG'GnC_Cy
Cuts at: 0 33 372 585
Size: 33 339 213
Fragments arranged by size:
339 213 33
EcoRI G'AATT_C
Cuts at: 0 562 585
Size: 562 23
HaeII r_GCGC'y
Cuts at: 0 574 585
Size: 574 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 585
Size: 22 32 531
Fragments arranged by size:
531 32 22
KasI G'GCGC_C
Cuts at: 0 570 585
Size: 570 15
MscI TGG'CCA
Cuts at: 0 190 451 585
Size: 190 261 134
Fragments arranged by size:
261 190 134
MslI CAynn'nnrTG
Cuts at: 0 169 585
Size: 169 416
MspA1I CmG'CkG
Cuts at: 0 132 182 240 306 440 585
Size: 132 50 58 66 134 145
Fragments arranged by size:
145 134 132 66 58 50
NarI GG'CG_CC
Cuts at: 0 571 585
Size: 571 14
NspI r_CATG'y
Cuts at: 0 558 585
Size: 558 27
PfoI T'CCnGG_A
Cuts at: 0 374 547 585
Size: 374 173 38
Fragments arranged by size:
374 173 38
PpuMI rG'GwC_Cy
Cuts at: 0 372 585
Size: 372 213
PstI C_TGCA'G
Cuts at: 0 368 585
Size: 368 217
PvuII CAG'CTG
Cuts at: 0 182 240 306 585
Size: 182 58 66 279
Fragments arranged by size:
279 182 66 58
SacII CC_GC'GG
Cuts at: 0 441 585
Size: 441 144
SapI GCTCTTCn'nnn_
Cuts at: 0 355 585
Size: 355 230
SfcI C'TryA_G
Cuts at: 0 177 364 585
Size: 177 187 221
Fragments arranged by size:
221 187 177
SfoI GGC'GCC
Cuts at: 0 572 585
Size: 572 13
SgrAI Cr'CCGG_yG
Cuts at: 0 166 585
Size: 166 419
SphI G_CATG'C
Cuts at: 0 558 585
Size: 558 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 281 585
Size: 281 304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 545 585
Size: 545 40
Enzymes that do cut:
AgeI AleI AlwNI ApoI AvaI BanI BanII BbeI
BbvCI BmgBI BpmI Bpu10I BsaHI BsaWI BseRI BseYI
BsgI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssHII BstYI
Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I
EcoRI HaeII Hin4I KasI MscI MslI MspA1I NarI
NspI PfoI PpuMI PstI PvuII SacII SapI SfcI
SfoI SgrAI SphI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BspMI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SgfI SmaI SmlI
SnaBI SpeI SrfI SspI StuI StyI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI