Restriction map for
pFN21AE6573
(Linear) (Six-base) MAPSORT of: KIEE6573.seq Check: 5261 from: 1 to: 603
KIEE6573.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 603
Size: 435 168
AfeI AGC'GCT
Cuts at: 0 582 603
Size: 582 21
AlwNI CAG_nnn'CTG
Cuts at: 0 397 603
Size: 397 206
ApaI G_GGCC'C
Cuts at: 0 508 603
Size: 508 95
ApaLI G'TGCA_C
Cuts at: 0 355 603
Size: 355 248
AvaI C'yCGr_G
Cuts at: 0 501 603
Size: 501 102
BanI G'GyrC_C
Cuts at: 0 43 424 487 595 603
Size: 43 381 63 108 8
Fragments arranged by size:
381 108 63 43 8
BanII G_rGCy'C
Cuts at: 0 508 603
Size: 508 95
BbeI G_GCGC'C
Cuts at: 0 491 603
Size: 491 112
BglI GCCn_nnn'nGGC
Cuts at: 0 394 603
Size: 394 209
Bme1580I G_kGCm'C
Cuts at: 0 359 508 603
Size: 359 149 95
Fragments arranged by size:
359 149 95
BmtI G_CTAG'C
Cuts at: 0 587 603
Size: 587 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 234 603
Size: 3 231 369
Fragments arranged by size:
369 231 3
Bpu10I CC'TnA_GC
Cuts at: 0 591 603
Size: 591 12
BsaHI Gr'CG_yC
Cuts at: 0 488 603
Size: 488 115
BsiHKAI G_wGCw'C
Cuts at: 0 359 603
Size: 359 244
BsmBI CGTCTCn'nnnn_
Cuts at: 0 476 603
Size: 476 127
Bsp1286I G_dGCh'C
Cuts at: 0 359 508 603
Size: 359 149 95
Fragments arranged by size:
359 149 95
BsrGI T'GTAC_A
Cuts at: 0 260 273 603
Size: 260 13 330
Fragments arranged by size:
330 260 13
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 603
Size: 27 576
ClaI AT'CG_AT
Cuts at: 0 308 603
Size: 308 295
EaeI y'GGCC_r
Cuts at: 0 383 546 603
Size: 383 163 57
Fragments arranged by size:
383 163 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 234 435 603
Size: 3 231 201 168
Fragments arranged by size:
231 201 168 3
EcoO109I rG'GnC_Cy
Cuts at: 0 462 505 603
Size: 462 43 98
Fragments arranged by size:
462 98 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 390 603
Size: 390 213
HaeII r_GCGC'y
Cuts at: 0 491 584 603
Size: 491 93 19
Fragments arranged by size:
491 93 19
KasI G'GCGC_C
Cuts at: 0 487 603
Size: 487 116
MspA1I CmG'CkG
Cuts at: 0 394 536 603
Size: 394 142 67
Fragments arranged by size:
394 142 67
NarI GG'CG_CC
Cuts at: 0 488 603
Size: 488 115
NheI G'CTAG_C
Cuts at: 0 583 603
Size: 583 20
NspI r_CATG'y
Cuts at: 0 459 603
Size: 459 144
PfoI T'CCnGG_A
Cuts at: 0 562 603
Size: 562 41
PpuMI rG'GwC_Cy
Cuts at: 0 462 603
Size: 462 141
PspOMI G'GGCC_C
Cuts at: 0 504 603
Size: 504 99
SfoI GGC'GCC
Cuts at: 0 489 603
Size: 489 114
SphI G_CATG'C
Cuts at: 0 459 603
Size: 459 144
StyI C'CwwG_G
Cuts at: 0 413 603
Size: 413 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 350 603
Size: 350 253
TatI w'GTAC_w
Cuts at: 0 119 260 273 603
Size: 119 141 13 330
Fragments arranged by size:
330 141 119 13
Enzymes that do cut:
AcuI AfeI AlwNI ApaI ApaLI AvaI BanI BanII
BbeI BglI Bme1580I BmtI BpmI Bpu10I BsaHI BsiHKAI
BsmBI Bsp1286I BsrGI BstXI ClaI EaeI Eco57MI EcoO109I
FalI HaeII KasI MspA1I NarI NheI NspI PfoI
PpuMI PspOMI SfoI SphI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI NaeI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI